Exosomal MiR-92a-3p serves as a Promising Diagnostic Marker and Potential Therapeutic Target for Adenomyosis

Exosomal MiR-92a-3p serves as a Promising Diagnostic Marker and Potential Therapeutic Target for Adenomyosis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Exosomal MiR-92a-3p serves as a Promising Diagnostic Marker and Potential Therapeutic Target for Adenomyosis Wanqi Shao, Yayuan Yu, Jianzhang Wang, Zhiruo Qiu, Shuyan Mei, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3961335/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 22 Mar, 2025 Read the published version in Scientific Reports → Version 1 posted 8 You are reading this latest preprint version Abstract This study aimed to elucidate the role of microRNA miR-92a-3p in the pathogenesis of adenomyosis. We focused on understanding how miR-92a-3p in exosomes derived from ectopic lesions influences the behavior of endometrial cells, DRG neurons, and Human Umbilical Vein Endothelial Cells (HUVECs), and its potential as a non-invasive diagnostic biomarker. Our findings revealed that MiR-92a-3p is significantly upregulated in exosomes derived from ectopic lesions of adenomyosis. This upregulation was associated with enhanced migration and invasion capabilities in eutopic endometrial cells, DRG neurons, and HUVECs. Furthermore, the study demonstrated a significant correlation between the levels of MiR-92a-3p in urinary exosomes and the clinical symptoms of adenomyosis, suggesting its potential as a non-invasive biomarker for the disease. This study elucidates an exosomal signaling process via miR-92a-3p that drives pathological infiltration and angiogenesis to promote adenomyosis progression. Upregulated miR-92a-3p in biofluid exosomes shows promising non-invasive biomarker potential for diagnosis and monitoring of this disease. Our findings unveil novel targets and tools for improved clinical management.Our research uncovers the significant role of miR-92a-3p in adenomyosis pathogenesis. We demonstrate that miR-92a-3p, upregulated in exosomes from ectopic lesions, enhances migration and invasion of endometrial cells, DRG neurons, and HUVECs. Crucially, miR-92a-3p levels in urinary exosomes correlate with adenomyosis symptoms, highlighting its potential as a non-invasive biomarker. This study offers new insights into adenomyosis progression and introduces novel diagnostic and therapeutic avenues. Biological sciences/Cell biology Health sciences/Biomarkers Health sciences/Diseases Health sciences/Pathogenesis Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 22 Mar, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 24 Jul, 2024 Reviews received at journal 03 May, 2024 Reviewers agreed at journal 22 Apr, 2024 Reviewers invited by journal 22 Apr, 2024 Editor assigned by journal 22 Apr, 2024 Editor invited by journal 02 Apr, 2024 Submission checks completed at journal 02 Apr, 2024 First submitted to journal 16 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3961335","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":286648255,"identity":"da01edd4-8da0-443d-a67d-51e9bc3cf779","order_by":0,"name":"Wanqi Shao","email":"","orcid":"","institution":"Zhejiang Chinese Medical University","correspondingAuthor":false,"prefix":"","firstName":"Wanqi","middleName":"","lastName":"Shao","suffix":""},{"id":286648256,"identity":"164676a4-7fd1-4e45-87de-05f410bcbb58","order_by":1,"name":"Yayuan Yu","email":"","orcid":"","institution":"Jiaxing University Affiliated Maternity and Child 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We focused on understanding how miR-92a-3p in exosomes derived from ectopic lesions influences the behavior of endometrial cells, DRG neurons, and Human Umbilical Vein Endothelial Cells (HUVECs), and its potential as a non-invasive diagnostic biomarker. Our findings revealed that MiR-92a-3p is significantly upregulated in exosomes derived from ectopic lesions of adenomyosis. This upregulation was associated with enhanced migration and invasion capabilities in eutopic endometrial cells, DRG neurons, and HUVECs. Furthermore, the study demonstrated a significant correlation between the levels of MiR-92a-3p in urinary exosomes and the clinical symptoms of adenomyosis, suggesting its potential as a non-invasive biomarker for the disease. This study elucidates an exosomal signaling process via miR-92a-3p that drives pathological infiltration and angiogenesis to promote adenomyosis progression. Upregulated miR-92a-3p in biofluid exosomes shows promising non-invasive biomarker potential for diagnosis and monitoring of this disease. Our findings unveil novel targets and tools for improved clinical management.Our research uncovers the significant role of miR-92a-3p in adenomyosis pathogenesis. We demonstrate that miR-92a-3p, upregulated in exosomes from ectopic lesions, enhances migration and invasion of endometrial cells, DRG neurons, and HUVECs. Crucially, miR-92a-3p levels in urinary exosomes correlate with adenomyosis symptoms, highlighting its potential as a non-invasive biomarker. 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