Standard Operating Protocol: Implantation of Cambrige NeuroTech chronic silicon probe and mini-amp-64 digital headstage in mice

Abstract In this protocol, all steps required to perform implantation of a Cambridge NeuroTech chronic silicon probe and mini-amp-64 digital headstage, for cerebellar single-unit electrophysiology (above Crus I/II) are described, alongside detailed pictures of the procedure. This protocol starts by describing the steps needed to assemble the probe and nano-Drive; required to adjust probe position in the brain once implanted. The protocol then proceeds into the surgical procedure itself, which highlights all the important steps to perform successful implantation by means of constructing a small, lightweight and user-configurable headpiece implant.