Effect of Intra-vaginal Bio-stimulation with artificial penis and Clitorial Massage to Increase the Pregnancy Rate of Water Buffalo in the Coastal Region of Bangladesh

The study aimed to determine the effect of intra-vaginal bio-stimulation with a modified penis-like device (mPLD) or artificial penis and clitorial massage subsequently artificial insemination (AI) to increase the pregnancy rate of water buffaloes. The study was conducted from July 2023 to June 2024 in the selected coastal region of Bangladesh. The AI was performed on 160 buffalo cows after observing their estrus sign. During AI, the buffalo cows were divided into four groups (group A: only AI was conducted; group B: intra-vaginal bio-stimulation with mPLD following AI; group C: massage of the clitoris after AI; group D: both mPLD and the clitorial massage was done following AI). Each group consisted of 40 animals. Age, parity, body weight, reproductive health status, history of previous calving difficulties, and estrus detection method were recorded. The overall pregnancy rate was found 41.3%. The pregnancy rate in groups A, B, C, and D were 32.5, 42.2, 37.5 and 52.5%, respectively. It is determined that both application of intra-vaginal bio-stimulation with mPLD and massage of the clitoris following AI (group D) showed a significant effect on pregnancy rate than the control group (group A). It is concluded that the application of mPLD in conjugation with massage of the clitoris enhances the pregnancy rate of buffalo cows.