Effect Of 5-Fluorouracil On The Expression Of Human Decorin Proteins "Invitro study"

Effect Of 5-Fluorouracil On The Expression Of Human Decorin Proteins "Invitro study" | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effect Of 5-Fluorouracil On The Expression Of Human Decorin Proteins "Invitro study" Khalida Kadhim, Syafinaz Amin-Nordin, Suet Lin Chia, Pheik-Sheen Cheow This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6519831/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objectives The objectives of this study were to focus on investigating the effect of the standard anti-cancer drug 5 Fluorouracil (5-FU) on the expression of human Decorin (HDCN) proteins, the calculation of half maximal inhibitory concentration (IC50), and selectivity index (SI) using the neoplastic model SW620 colorectal adenocarcinoma cell line as compared with the normal human embryonic kidney (HEK293) cell line using the flow cytometry technique. Materials and Methods The expression of HDCN was investigated in both SW620 and HEK293 cell lines after 24, 48 and 72 hours of 5-FU treatment by achievement of a flow cytometry assay using specific HDCN antibodies. IC50 and SI concerning HDCN expression were calculated for 5-FU. Results The preliminary RCSB PDB findings revealed that h uman DCN generally contains four groups of ligands, including acetate, 1, 2-Ethanediol or ethylene glycol, phosphate, and sulfate ions. Virtual docking revealed classic hydrogen bonds between Ser111 (chain B) and Pro67 (chain A). A halogen bond was observed in reference to Leu109 (chain B). π-Alkyl, van der Waals, and carbon-hydrogen interactions were also observed. The IC50 showed considerable results against SW620 cells, with a positive safety margin against HEK293 cells. The values of IC50 for 5-FU after 24, 48 and 72 were respectively recorded as 0.449µg/ml, 3.07µg/ml and 0.498µg/ml on SW620 cell line, and 0.46µg/ml, 33.113µg/ml and 12.882µg/ml on HEK293 cells. The therapeutic selectivity index was exponentially elevated and positively correlated with the incubation intervals, being equal to 1.024, 10.786 and 25.867 respectively, after 24, 48 and 72 hours of 5-FU treatment. Conclusion Human DCN was up regulated in SW620 cell line after treatment with 5-fluorouracil but the latter still has a positive therapeutic index concerning the safety margin toward HEK293 cell line. Decorin. Flow cytometry. HDCN expression Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryExcellfileforreviewersonly.xlsx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6519831","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":468823246,"identity":"c05f3231-29fa-41e3-81c8-fc481c59ce89","order_by":0,"name":"Khalida Kadhim","email":"data:image/png;base64,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","orcid":"","institution":"University of Kufa","correspondingAuthor":true,"prefix":"","firstName":"Khalida","middleName":"","lastName":"Kadhim","suffix":""},{"id":468823247,"identity":"8cbee246-8e73-444c-ac89-8fdb0eb3f743","order_by":1,"name":"Syafinaz Amin-Nordin","email":"","orcid":"","institution":"Universiti Putra Malaysia","correspondingAuthor":false,"prefix":"","firstName":"Syafinaz","middleName":"","lastName":"Amin-Nordin","suffix":""},{"id":468823248,"identity":"d30c7360-5e11-4aa5-b01e-a5e6ac428a81","order_by":2,"name":"Suet Lin Chia","email":"","orcid":"","institution":"Universiti Putra Malaysia","correspondingAuthor":false,"prefix":"","firstName":"Suet","middleName":"Lin","lastName":"Chia","suffix":""},{"id":468823249,"identity":"1853bbbd-5830-413f-a3d5-3dc3c312030c","order_by":3,"name":"Pheik-Sheen Cheow","email":"","orcid":"","institution":"Universiti Putra Malaysia","correspondingAuthor":false,"prefix":"","firstName":"Pheik-Sheen","middleName":"","lastName":"Cheow","suffix":""}],"badges":[],"createdAt":"2025-04-24 10:23:28","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6519831/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6519831/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":85291263,"identity":"65e5a30d-91e9-4ed2-8619-58db6da6674f","added_by":"auto","created_at":"2025-06-24 10:02:16","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":889842,"visible":true,"origin":"","legend":"","description":"","filename":"Drkhalida1stresearch2352025.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6519831/v1_covered_96bbed84-b33b-479f-a84c-5dc259a775e0.pdf"},{"id":84309823,"identity":"dbf2712a-197e-4c17-a2be-a55f72aa2a9a","added_by":"auto","created_at":"2025-06-10 12:11:22","extension":"xlsx","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":142157,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryExcellfileforreviewersonly.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-6519831/v1/b38c6dd8e734fc0fb80b4a62.xlsx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Effect Of 5-Fluorouracil On The Expression Of Human Decorin Proteins \"Invitro study\"","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Decorin. Flow cytometry. HDCN expression","lastPublishedDoi":"10.21203/rs.3.rs-6519831/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6519831/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eObjectives\u003c/h2\u003e \u003cp\u003eThe objectives of this study were to focus on investigating the effect of the standard anti-cancer drug 5 Fluorouracil (5-FU) on the expression of human Decorin (HDCN) proteins, the calculation of half maximal inhibitory concentration (IC50), and selectivity index (SI) using the neoplastic model SW620 colorectal adenocarcinoma cell line as compared with the normal human embryonic kidney (HEK293) cell line using the flow cytometry technique.\u003c/p\u003e\u003ch2\u003eMaterials and Methods\u003c/h2\u003e \u003cp\u003eThe expression of HDCN was investigated in both SW620 and HEK293 cell lines after 24, 48 and 72 hours of 5-FU treatment by achievement of a flow cytometry assay using specific HDCN antibodies. IC50 and SI concerning HDCN expression were calculated for 5-FU.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eThe preliminary RCSB PDB findings revealed that \u003cb\u003eh\u003c/b\u003euman DCN generally contains four groups of ligands, including acetate, 1, 2-Ethanediol or ethylene glycol, phosphate, and sulfate ions. Virtual docking revealed classic hydrogen bonds between Ser111 (chain B) and Pro67 (chain A). A halogen bond was observed in reference to Leu109 (chain B). π-Alkyl, van der Waals, and carbon-hydrogen interactions were also observed. The IC50 showed considerable results against SW620 cells, with a positive safety margin against HEK293 cells. The values of IC50 for 5-FU after 24, 48 and 72 were respectively recorded as 0.449\u0026micro;g/ml, 3.07\u0026micro;g/ml and 0.498\u0026micro;g/ml on SW620 cell line, and 0.46\u0026micro;g/ml, 33.113\u0026micro;g/ml and 12.882\u0026micro;g/ml on HEK293 cells. 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