Effects of miR-629-5p on odontoblast differentiation and target gene TEAD4 expression of dental pulp stem cells

Effects of miR-629-5p on odontoblast differentiation and target gene TEAD4 expression of dental pulp stem cells | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effects of miR-629-5p on odontoblast differentiation and target gene TEAD4 expression of dental pulp stem cells Jianpeng Han, Xin Zhao, Rongrong Jiang, Chengfeng Jiang, lei li, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1963908/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract TEAD4 is a member of the TEA domain (TEAD) family of transcription factors. It plays a key regulatory role in embryonic development, tissue homeostasis and cancer progression. Its expression is related to the regulation of a variety of inflammations. MicroRNA can regulate the expression of target genes and play an important role in various physiological and pathological processes. In view of the important role of TEAD4 and microRNA-629-5p (miR-629-5p) in inflammation, and based on the findings of bioinformatics research, we selected miR-629-5p as the focus of our study. In inflammatory dental pulp, we found that the expression of miR-629-5p was increased, while the expression of TEAD4 was decreased. We used Porphyromonas gingivalis lipopolysaccharide (LPS) as a stimulator of dental pulp stem cells (DPSCs) to simulate the inflammatory environment of dental pulp. The mineralization ability of LPS-stimulated DPSCs was significantly inhibited, while the level of miR-629-5p increased and the level of TEAD4 decreased. Inhibition of miR-629-5p can reverse the odontogenic defects of DPSCs treated with LPS. In addition, the expression of miR-629-5p in DPSCs was negatively correlated with the expression of TEAD4. In conclusion, miR-629-5p can inhibit the odontogenic differentiation of human dental pulp stem cells and the mechanism may be related to its role in downregulation of TEAD4 expression. MicroRNA-629 5P Dental pulp stem cells (DPSCs) TEAD4 Odontoblastic differentiation Deep caries Reversible pulpitis Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1963908","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":129626017,"identity":"7e7662e1-c11a-4ea9-b05c-ead216f12cfe","order_by":0,"name":"Jianpeng Han","email":"","orcid":"","institution":"Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong)","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jianpeng","middleName":"","lastName":"Han","suffix":""},{"id":129626018,"identity":"cc1d905f-e68c-42e3-a735-e1fd185a4d9a","order_by":1,"name":"Xin 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It plays a key regulatory role in embryonic development, tissue homeostasis and cancer progression. Its expression is related to the regulation of a variety of inflammations. MicroRNA can regulate the expression of target genes and play an important role in various physiological and pathological processes. In view of the important role of TEAD4 and microRNA-629-5p (miR-629-5p) in inflammation, and based on the findings of bioinformatics research, we selected miR-629-5p as the focus of our study. In inflammatory dental pulp, we found that the expression of miR-629-5p was increased, while the expression of TEAD4 was decreased. We used Porphyromonas gingivalis lipopolysaccharide (LPS) as a stimulator of dental pulp stem cells (DPSCs) to simulate the inflammatory environment of dental pulp. The mineralization ability of LPS-stimulated DPSCs was significantly inhibited, while the level of miR-629-5p increased and the level of TEAD4 decreased. Inhibition of miR-629-5p can reverse the odontogenic defects of DPSCs treated with LPS. In addition, the expression of miR-629-5p in DPSCs was negatively correlated with the expression of TEAD4. 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