Microtubule dynamics in adult retinal ganglion cells (RGCs) and dorsal root ganglion (DRG) neurons.

Microtubule dynamics in adult retinal ganglion cells (RGCs) and dorsal root ganglion (DRG) neurons. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Microtubule dynamics in adult retinal ganglion cells (RGCs) and dorsal root ganglion (DRG) neurons. Elena Vecino This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7298159/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract While axon regeneration is very limited in the adult central nervous system (CNS) in vivo , this is not the case in the peripheral nervous system (PNS). Indeed, both CNS and PNS neurons can regenerate in vitro although to varying degrees. Given the role of microtubule stabilization in promoting regeneration, we have examined microtubule polymerization during the regeneration of two types of adult neurons in vitro , retinal ganglion cells (RGCs) from the CNS and dorsal ganglion (DRG) neurons from the PNS. In order to compare microtubule dynamics between these cell types, the density, polymerization rate and orientation of microtubules have been analysed during neurite regeneration in both cell types by analysing GFP-tagged Microtubule End Binding 3 (EB3) protein transfected into the neurons . The density of EB3 comets and the speed of EB3 movement was similar in both cell types, although only one subtype of RGC regenerated sufficiently long neurites for analysis. In the absence of extracellular substances that could inhibit neurite regeneration, the dynamics of the microtubules of the RGC subtype that extend long neurites are very similar to those in DRG neurons. However, some RGCs with very short neurites exhibited EB3 comets that progressed retrogradely. Additionally, live imaging of mitochondria was performed in both neuronal cultures. In summary, our findings show that regenerating neurites from the CNS and PNS share similar microtubule extension dynamics. Importantly, the observation that only certain RGC subtypes demonstrate robust regenerative capacity highlights the need to account for the molecular diversity among RGCs in future studies. Cellular & Molecular Neuroscience retinal ganglion cells dorsal ganglion neurons microtubules neuroregeneration EB3 retina vision Full Text Additional Declarations The authors declare no competing interests. The Supplementary Videos are not available with this version. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7298159","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":495881339,"identity":"3df81e0d-be94-4dcc-8195-c158721ec2fb","order_by":0,"name":"Elena Vecino","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA4ElEQVRIiWNgGAWjYDACdhiDGYg/MBwgQgszEoNxBmlaQGweYrTwMzMfe/CD4bAcfzvvwcc2FXcSG9h7jD/g0yLZzJZu2MNw2FjiMF+ycc6ZZ4kNPGfMJPBpMTjMYybBw5CWuIGZx0w6t+1wYoNEjhleh9kf5v8m+YchrR6sxfIfUIv8G/wOM2DmYZPmYbBJMABpYWwA2cJjgNdhEofZzKRlDGwMZxzmMTbsOXbYuI0nrQyvFv725meSbyok5Pn7zxg++FFzWLaf/fBmvA6DOg+JzUZY+SgYBaNgFIwCQgAAL5Q+SupmluIAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0002-1672-5132","institution":"University of the Basque Country UPV/EHU","correspondingAuthor":true,"prefix":"","firstName":"Elena","middleName":"","lastName":"Vecino","suffix":""}],"badges":[],"createdAt":"2025-08-05 08:30:51","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":true,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":true},"doi":"10.21203/rs.3.rs-7298159/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7298159/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":88577414,"identity":"c87d61cb-31b6-40b7-b3b6-fd628d4aee6a","added_by":"auto","created_at":"2025-08-08 02:13:58","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1146162,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptEB3shortreport.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7298159/v1_covered_3a169047-22bb-4fa1-9189-468d2fae9205.pdf"}],"financialInterests":"\u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e\n\u003cp\u003eThe Supplementary Videos are not available with this version.\u003c/p\u003e","formattedTitle":"\u003cp\u003e\u003cstrong\u003eMicrotubule dynamics in adult retinal ganglion cells (RGCs) and dorsal root ganglion (DRG) neurons.\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[{"identity":"79dda0de-6a93-48a1-b84f-ca65de69fed3","identifier":"10.13039/501100003086","name":"Eusko Jaurlaritza","awardNumber":"IT1510-22","order_by":0},{"identity":"259744a3-662b-4142-adcf-c44e8cb94942","identifier":"10.13039/501100015498","name":"Ministry for Innovation and Technology","awardNumber":"AEI/10.13039/501100011033","order_by":1}],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"University of Cambridge (UK)","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"retinal ganglion cells, dorsal ganglion neurons, microtubules, neuroregeneration, EB3, retina, vision","lastPublishedDoi":"10.21203/rs.3.rs-7298159/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7298159/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eWhile axon regeneration is very limited in the adult central nervous system (CNS) \u003cem\u003ein vivo\u003c/em\u003e, this is not the case in the peripheral nervous system (PNS). 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