Shatavari Supplementation in Postmenopausal Women Alters the Skeletal Muscle Proteome and Pathways Involved in Training Adaptation

Shatavari Supplementation in Postmenopausal Women Alters the Skeletal Muscle Proteome and Pathways Involved in Training Adaptation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Shatavari Supplementation in Postmenopausal Women Alters the Skeletal Muscle Proteome and Pathways Involved in Training Adaptation Mary O'Leary, Sarah R Jackman, Joanna L Bowtell This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-2369451/v2 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 12 Jan, 2024 Read the published version in European Journal of Nutrition → Version 2 posted You are reading this latest preprint version Show more versions Abstract Purpose: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari’s actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples that remained from our original study. Methods: In a randomised double-blind trial, women (68.5 ± 6 years) ingested either placebo or shatavari (equivalent to 26,500 mg/d fresh weight) for six weeks. Tandem mass tag global proteomic analysis of VL samples was conducted (N=7 shatavari, N=5 placebo). Data were normalised to total peptides and scaled using a reference sample. Data were filtered using a 5% FDR. For each protein, the pre to post supplementation difference was expressed as log2 fold change. Welch’s t tests with Benjamini-Hochberg corrections were performed for each protein. Pathway enrichment (PADOG, CAMERA) was interrogated in Reactome (v85). Results: No individual protein was significantly different between supplementation conditions. Both PADOG and CAMERA indicated that pathways related to 1) Integrin/MAPK signalling, 2) metabolism/insulin secretion; 3) cell proliferation/senescence/DNA repair/cell death; 4) haemostasis/platelets/fibrin; 5) signal transduction; 6) neutrophil degranulation and 7) chemical synapse function were significantly upregulated. CAMERA indicated pathways related to translation/amino acid metabolism, viral infection, and muscle contraction were downregulated. Conclusion: Our analyses indicate that shatavari may support muscle adaptation responses to exercise. These data provide useful signposts for future investigation of shatavari’s utility in conserving and enhancing musculoskeletal function in older age. Trial registration NCT05025917 30/08/21, retrospectively registered. Full Text Supplementary Files OnlineResource1.xlsx OnlineResource2.docx OnlineResource3PADOGReactomeOutput.xlsx Onlineresource4CAMERAReactomeoutput.xlsx PUK1RawDataProteomics.xlsx Cite Share Download PDF Status: Published Journal Publication published 12 Jan, 2024 Read the published version in European Journal of Nutrition → Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari’s actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples that remained from our original study.\nMethods: In a randomised double-blind trial, women (68.5 ± 6 years) ingested either placebo or shatavari (equivalent to 26,500 mg/d fresh weight) for six weeks. Tandem mass tag global proteomic analysis of VL samples was conducted (N=7 shatavari, N=5 placebo). Data were normalised to total peptides and scaled using a reference sample. Data were filtered using a 5% FDR. For each protein, the pre to post supplementation difference was expressed as log2 fold change. Welch’s t tests with Benjamini-Hochberg corrections were performed for each protein. 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