Platelet Rich Fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Objectives: Pre-Vascularization of collagen membranes with autologous platelet concentrates is a standard procedure in oral and maxillofacial surgery. This study analyzed the possible interaction of an acellular collagen membrane of porcine origin (NM) with platelet-rich fibrin (PRF) regarding its rehydration protocol with differences in pH values and effect on angiogenesis. Materials: and methods NM was analyzed alone and combined with solid PRF by plotting or co-culturing with injectable PRF. Different media (venous blood, buffer solution with a fixed pH value of 7, saline solution, and injectable PRF) were used to analyze the influence on pH value during rehydration. Chorion allantois membrane assay (CAM) was applied to check pro-angiogenic effects after 24, 48, and 72 hours, followed by immunohistochemical analysis. Results: Rehydration in injectable PRF showed acidity over time (p 0.005). This pro-angiogenic effect could not be exaggerated when NM was combined with liquid/solid PRF (each p>0.005). Conclusions: Rehydration with liquid PRF of the collagen membrane results in acidity compared to a saline solution or patient’s blood. The significant pro-angiogenic potential of the membrane alone resulted in enhanced neo-vessel formation that could not be optimized with the addition of PRF.