p53 in Endometriosis

Toshihiko Toki
In: International Journal of Gynecological Pathology · 2002 · vol. 21(1) , pp. 93 · doi:10.1097/00004347-200201000-00020 · W1968595655
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This study confirmed the specificity of p53 staining in endometriosis by using negative controls and blocking endogenous biotin activity.

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This brief correspondence addresses a methodological concern regarding the specificity of p53 immunostaining in endometriosis tissue samples. The author confirms that parallel sections stained with normal mouse serum served as negative controls and showed no staining, thereby validating the absence of non-specific binding. Furthermore, preincubation with streptavidin did not reduce positive signals, ruling out interference from endogenous biotin activity and supporting the conclusion that the observed p53 staining is specific to the target antigen. This paper is centrally about endometriosis — specifically addressing technical validation of p53 detection methods in endometriotic lesions.

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Abstract

I thank Dr. Mittal for indicating a very important point. We stained parallel sections using normal mouse serum (instead of anti-p53 antibody) as a negative control, and no staining was observed in the sections that showed positive p53 staining. We further examined the possibility of endogenous biotin activity by preincubating the sections with streptavidin (Pierce, Rockford, IL). Because the positive p53 staining was not reduced or lost even after this blocking test, we believe that the positive p53 staining is specific and not due to endogenous biotin activity. Toshihiko Toki, M.D.
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I thank Dr. Mittal for indicating a very important point. We stained parallel sections using normal mouse serum (instead of anti-p53 antibody) as a negative control, and no staining was observed in the sections that showed positive p53 staining. We further examined the possibility of endogenous biotin activity by preincubating the sections with streptavidin (Pierce, Rockford, IL). Because the positive p53 staining was not reduced or lost even after this blocking test, we believe that the positive p53 staining is specific and not due to endogenous biotin activity. Toshihiko Toki, M.D.Letter To The Editor p53 in Endometriosis - Toshihiko Toki International Journal of Gynecological Pathology 21(1):p 93, January 2002. Copyright © 2002 Lippincott Williams & Wilkins, Inc.

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endometriosis

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