Heparanase and metastin expressions in eutopic endometrial glandular epithelium cells of adenomyosis in vitro and the effect of mifepristone for their expressions and significance

Objective To detect the expression of heparanase,Kiss-1 and metastin in eutopic endometrial glandular cells of adenomyosis in vitro,and the effects of mifepristone on the expression,in order to investigate the effects of mifepristone on the intervention mechanism of invasive factors in adenomyosis. Methods 32 cases of patients were pathologically diagnosed as adenomyosis at the First Hospital of Shanxi Medical University between July 2007 and December 2008,using RT-PCR and Western-blot to detect the HPA and Kiss-1 gene and the HPA and metastin protein expression in eutopic endometrial glandular cells of adenomyosis in vitro,and intervening with mifepristone. Results The expression of HPA mRNA in adenomyosis group proliferative and secretory phase glandular epithelial cells were 1.39 ± 0.05 and 1.28 ± 0.04 respectively,higher than control group (P0.05). Kiss-1 mRNA expression were 0.78 ± 0.07 and 0.82 ± 0.05 respectively,lower than the control group (P0.05). However,there is no statistical significance between proliferative and secretory phase within the same group (P0.05). The levels of protein expression of HPA and metastin showed consistent with the gene expression. After drug intervention on adenomyosis group,HPA mRNA and protein expression decreased,and Kiss-1 mRNA and protein expression metastin increased,which means obvious differences compared to pre-treatment (P0.05). Conclusion The imbalance expression of invasive factors (HPA) and invasive inhibiting factors (metastin) in endometrial glandular epithelial cells may be one of the pathogenesis; the intervention of mifepristone on the above two factors may be one of the inhibitive mechanisms on adenomyosis development.