Probing intracellular yeast metabolism with deuterium magnetic resonance spectroscopy

Probing intracellular yeast metabolism with deuterium magnetic resonance spectroscopy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Probing intracellular yeast metabolism with deuterium magnetic resonance spectroscopy Fatima Anum, Charbel Assaf, Farhad Haj Mohamad, Maria Anikeeva, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5312977/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Metabolomics provides insights into biochemical dynamics, with nuclear magnetic resonance (NMR) being one of the few noninvasive techniques. However, traditional 1H NMR often suffers from overlapping signals from proteins and numerous metabolites. To address this, we employed deuterium labeling, reducing background interference and streamlining metabolic analysis. Deuterium magnetic resonance spectroscopy (DMRS) enables rapid, noninvasive measurement of metabolic flux without specialized equipment. In our study, we first measured T1, T2, and chemical shifts for 25 deuterium-labeled compounds in phosphate-buffered saline: parameters functional for optimal DMRS settings. Among the 25 deuterated compounds tested with food-grade baker’s yeast (Saccharomyces cerevisiae) as a model solution, we observed and tracked the real-time consumption of pyruvate, glucose, fumarate, acetone, and nicotinamide. We redirected yeast metabolism by (i) varying concentrations of added pyruvate and (ii) osmotic pressure by changing buffer density. This study underscores DMRS’s potential as a robust, versatile tool for dissecting metabolic transformations exemplified here with the convenient yeast cell systems active for hundreds of minutes under typical NMR observation conditions. Biological sciences/Biochemistry/Metabolomics Biological sciences/Biological techniques/Analytical biochemistry/Biochemical assays Biological sciences/Chemical biology/Metabolic pathways Biological sciences/Chemical biology/Chemical tools Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Supportinginformation.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5312977","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":369198704,"identity":"01a0cab4-ca8a-47a2-bd6e-4419b9ac9498","order_by":0,"name":"Fatima Anum","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Fatima","middleName":"","lastName":"Anum","suffix":""},{"id":369198705,"identity":"a01794f4-1414-43a4-a988-263fae302b4a","order_by":1,"name":"Charbel Assaf","email":"","orcid":"","institution":"Kiel University","correspondingAuthor":false,"prefix":"","firstName":"Charbel","middleName":"","lastName":"Assaf","suffix":""},{"id":369198706,"identity":"f1c55172-5dee-497b-8e05-991ba46a5861","order_by":2,"name":"Farhad Haj Mohamad","email":"","orcid":"","institution":"Kiel University","correspondingAuthor":false,"prefix":"","firstName":"Farhad","middleName":"Haj","lastName":"Mohamad","suffix":""},{"id":369198707,"identity":"860f217d-dd45-45bb-8db8-954ca6186f8f","order_by":3,"name":"Maria Anikeeva","email":"","orcid":"","institution":"Kiel University","correspondingAuthor":false,"prefix":"","firstName":"Maria","middleName":"","lastName":"Anikeeva","suffix":""},{"id":369198708,"identity":"017a7cf9-1760-46fa-aa10-cbcb926716b9","order_by":4,"name":"Arne Brahms","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Arne","middleName":"","lastName":"Brahms","suffix":""},{"id":369198709,"identity":"94573ec3-1e56-47a6-af9b-b4d2ba71ba4d","order_by":5,"name":"Jyotirmoy Dey","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Jyotirmoy","middleName":"","lastName":"Dey","suffix":""},{"id":369198710,"identity":"88cb9d2b-7563-434f-b757-e0252925a905","order_by":6,"name":"Simon Kaltenberger","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Simon","middleName":"","lastName":"Kaltenberger","suffix":""},{"id":369198711,"identity":"945916bc-afe4-4b46-978a-0c259fe726cd","order_by":7,"name":"Eric Beitz","email":"","orcid":"https://orcid.org/0000-0001-5912-6626","institution":"Pharmaceutical Institute, CAU Kiel","correspondingAuthor":false,"prefix":"","firstName":"Eric","middleName":"","lastName":"Beitz","suffix":""},{"id":369198712,"identity":"e1284212-1073-4390-a4bc-37451c4e6719","order_by":8,"name":"Lina Welz","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Lina","middleName":"","lastName":"Welz","suffix":""},{"id":369198713,"identity":"11e9fbc3-be54-41f1-a82c-62a1fadaaabd","order_by":9,"name":"Victoria Annis","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Victoria","middleName":"","lastName":"Annis","suffix":""},{"id":369198714,"identity":"b5ce8ebc-ad40-4cd0-a2a8-530dbbf05e3e","order_by":10,"name":"Manuel van Gemmeren","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Manuel","middleName":"van","lastName":"Gemmeren","suffix":""},{"id":369198715,"identity":"6b1910fc-add8-431d-89c0-5f4d5a94f495","order_by":11,"name":"Simon Duckett","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Simon","middleName":"","lastName":"Duckett","suffix":""},{"id":369198716,"identity":"7e81cc92-e685-4970-96ed-1a6a41d671f2","order_by":12,"name":"Jan-Bernd Hövener","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Jan-Bernd","middleName":"","lastName":"Hövener","suffix":""},{"id":369198703,"identity":"06812e22-3848-498c-bfd7-ca06d24e872d","order_by":13,"name":"Andrey N. 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