GO/Alg/PRP Regulates Oxidative Stress Through the p38MAPK/NF-κB Pathway on Ischemic Reperfusion Pressure Injury in Mice

Pressure Injury（PI） are localized damages to the skin or underlying soft tissues typically caused by intense and/or prolonged pressure, shearing, friction, or combination of these factors. The wound is difficult to heal and easily leads to infection and other complications. The repair of PI has become a global health problem. This paper describes the preparation and characterization of Graphene Oxide (GO)/Alginate (Alg) gel-loaded Platelet-Rich Plasma (PRP), and the effects of GO/Alg/PRP on cell proliferation and angiogenesis have been evaluated by cell experiments. The effects of GO/Alg/PRP on the Ischemic Reperfusion (I/R) model in mice were evaluated in animal experiments, and the relationship between oxidative stress and p38 Mitogen-Activated Protein Kinase (P38MAPK)/ Nuclear Factor-κB (NF-κB) pathway was investigated. GO/Alg, PRP and GO/Alg/PRP all promote cell proliferation, migration, angiogenesis, wound healing, collagen deposition and epithelial regeneration, and reduce oxidative stress damage of cells and tissues. Among these, GO/Alg/PRP plays the most significant role. We also found a relationship between oxidative stress and the p38MAPK/NF-κB pathway. Our findings suggest that GO/Alg/PRP could be an effective strategy for the treatment of I/R injuries and may serve as a basis for the development of novel PRP based bioactive wound dressings.