Thermophiles in Nanosized Biocalcification: a Novel Approach for Heavy metal Remediation  

Thermophiles in Nanosized Biocalcification: a Novel Approach for Heavy metal Remediation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Thermophiles in Nanosized Biocalcification: a Novel Approach for Heavy metal Remediation Sujata Negi, Shagun Sharma, Deepak Pant, Sonali Sharma, Kalpana Chauhan, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5900773/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Biodeposition of minerals is a prevalent occurrence in the biological realm, facilitated by various organisms such as bacteria, protists, fungi, and plants. Calcium carbonate is one such mineral that precipitates naturally as a consequence of microbial metabolic processes. This study investigates an innovative approach for carbon dioxide (CO2) sequestration by utilizing thermophilic microorganisms (bacteria and enzymes) in the conversion of CO 2 into CaCO3 and methanol. The thermophilic bacterial strains of Bacillus licheniformis and enzymes formaldehyde dehydrogenase (FalDH) and alcohol dehydrogenase (ADH) were screened for their ability of microbial-induced carbonate precipitation (MICP). The research focuses on identifying the various optimal conditions, such as CO2 concentration, bacterial concentration, pH, time, and temperature, for CaCO3 biosynthesis. Both strains of Bacillus licheniformis found to be the most efficient in biocalcification. Notably, these microbes also redirected their great potential towards the heavy metal remediation strategy. Heavy metals are non-biodegradable, and as they persist in the environment, they accumulate and magnify in ecosystems, intensifying the harmful effects on living organisms. Thermophilic bacterial strain excellently removes Pb (II) ions from aqueous solutions of MICP with 90% efficiency, and nanosized (35.85 nm, 38.58 nm) biominerals were formed. These comparative findings provide valuable insights for optimizing bio-based carbonate production in CO2 sequestration technology. Biodeposition bio-based Heavy metal Remediation biominerals Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5900773","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":408985392,"identity":"882936c1-cb27-4e32-a165-1a9bcd293b5d","order_by":0,"name":"Sujata Negi","email":"","orcid":"","institution":"Central University of Haryana","correspondingAuthor":false,"prefix":"","firstName":"Sujata","middleName":"","lastName":"Negi","suffix":""},{"id":408985395,"identity":"88918ef8-3142-4958-9db2-5d73e5ab2ccf","order_by":1,"name":"Shagun Sharma","email":"","orcid":"","institution":"Indian Institute of Technology 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