Machine Learning Discoveries of GINS1-X Synergy in ETC-1922159 Treated Colorectal Cancer Cells

GINS1 (PSF1 homolog) forms a part of complex called GINS, named after numbers 5-1-2-3 (go-ichi-ni-san in Japanese) apropos the 4 protein subunits of the complex, namely, SLD5, PSF1, PSF2, and PSF3. It is a subgroup of the CDC45-MCM-(2 to 7)- GINS (CMG) complex, the enzyme that unwinds double-stranded DNA at replication forks. GINS1 has been found to be overexpressed in multiple cancers. In colorectal cancer (CRC) cells treated with ETC-1922159, GISN1 was found to be down regulated along with other genes. A recently developed search engine ranked combinations of GISN1-X (X, a particular gene/protein) at 2nd order level after drug administration. Some of these combinations have been tested in wet lab, however many have been pointed out by the search engine that are yet to be explored/tested. These rankings reveal which GINS1-X combinations might be working synergistically in CRC. In this research work, I cover combinations of GINS1 with possible members of, origin recognition complex (ORC), minichromosome maintenance complex (MCM), DNA- directed DNA polymerases (POL), DNA-dependent RNA polymerase (POLR), topoisomerases (TOP), ubiquitin specific peptidase (USP), Fanconi anemia complementa- tion group (FANC), forkhead box (FOX) and notch receptor (NOTCH) family.