Bioleaching of Coal Gangue and Residual Coal by Ochrobactrum sp. - B2: Enhancing Silica Extraction and Mitigating Toxicity in Coal Tailings

Bioleaching of Coal Gangue and Residual Coal by Ochrobactrum sp. - B2: Enhancing Silica Extraction and Mitigating Toxicity in Coal Tailings | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Bioleaching of Coal Gangue and Residual Coal by Ochrobactrum sp. - B2: Enhancing Silica Extraction and Mitigating Toxicity in Coal Tailings Qingshan Zhang, Xiaoman Luo, Xinxin Yan, Yaoli Peng This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6129029/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Coal tailings are typical by-products of coal preparation plants, primarily consisting of coal gangue and bio toxic residual coal. The rational utilization of coal gangue and treatment of residual coal pose significant environmental challenges. In this study, five strains with silicon activation capabilities were isolated from coal tailings, and a strain named Ochrobactrum sp. B2 was selected and purified. Using nitrosoguanidine (NTG) mutagenesis, a strain B2-M with stronger adaptability to coal tailings was obtained. Based on its metabolic characteristics, the conditions for biological leaching were optimized, resulting in an available silicon concentration in the leachate reaching 320.54 mg/L. Through direct and indirect leaching experiments, the adhesion and synergistic leaching mechanisms between bacteria and coal tailings were investigated. Kinetic results indicated that the biological leaching process follows chemical reaction control. FT-IR and GC-MS analyses demonstrated that the B2-M strain could disrupt and decompose the residual coal in coal tailings, converting it into utilizable small molecule organics and thus enhancing the leaching efficiency of available silicon from coal gangue. The combined capabilities of the B2-M strain for decomposing residual coal and leaching available silicon reduced the toxicity of polycyclic aromatic hydrocarbons in coal tailings, significantly improving the green utilization efficiency of coal tailings. Bioleaching Biodegradability Ochrobactrum Sp Mutagenesis Available Silicon kinetics Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6129029","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":423357175,"identity":"bee3dde7-b908-499a-a2d5-adb519bd6ee1","order_by":0,"name":"Qingshan Zhang","email":"","orcid":"","institution":"China University of Mining and Technology","correspondingAuthor":false,"prefix":"","firstName":"Qingshan","middleName":"","lastName":"Zhang","suffix":""},{"id":423357177,"identity":"bf4968fe-083b-47c2-a8ee-a52665c61d19","order_by":1,"name":"Xiaoman Luo","email":"","orcid":"","institution":"China University of Mining and Technology","correspondingAuthor":false,"prefix":"","firstName":"Xiaoman","middleName":"","lastName":"Luo","suffix":""},{"id":423357180,"identity":"aa3b7688-38f9-4499-b3aa-2171aadadbb1","order_by":2,"name":"Xinxin Yan","email":"","orcid":"","institution":"Institute of Geochemistry Chinese Academy of Science","correspondingAuthor":false,"prefix":"","firstName":"Xinxin","middleName":"","lastName":"Yan","suffix":""},{"id":423357188,"identity":"fcfe87b7-dfcb-4db1-a5ca-a33c35f1299e","order_by":3,"name":"Yaoli Peng","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA0ElEQVRIiWNgGAWjYDADPmbmAwc+/CBFCxszW+LBmT0kaWHgMT7MwUaESnP23sMvGNvs5NjYeT4cZuBhkOcXO4Bfi2XPuTQLxrZkYzZm3g2HCywYDGfOTsCvxeBGjpkBYxtzYhtIywwehgSD24S03H8D0lIP1MLz4DAPGzFabvAYP2BsOwzSwkCcFsueHDMGhnPHgX5hMwAGsgRhv5iznzH+wFBWLcfPf/jxhw8/bOT5pQk5DBgj0n8R0SGBXzlUC/MHhj+EFY6CUTAKRsEIBgCqfj388Cn/RQAAAABJRU5ErkJggg==","orcid":"","institution":"China University of Mining and Technology","correspondingAuthor":true,"prefix":"","firstName":"Yaoli","middleName":"","lastName":"Peng","suffix":""}],"badges":[],"createdAt":"2025-02-28 13:38:11","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6129029/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6129029/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":82070119,"identity":"6cecccb2-bb02-4ec2-a949-980ba2f0f988","added_by":"auto","created_at":"2025-05-06 13:09:16","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1169665,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6129029/v1_covered_122f0e8f-8a35-4f7d-b68b-90111781ace6.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Bioleaching of Coal Gangue and Residual Coal by Ochrobactrum sp. - B2: Enhancing Silica Extraction and Mitigating Toxicity in Coal Tailings","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Bioleaching, Biodegradability, Ochrobactrum Sp, Mutagenesis, Available Silicon, kinetics","lastPublishedDoi":"10.21203/rs.3.rs-6129029/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6129029/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eCoal tailings are typical by-products of coal preparation plants, primarily consisting of coal gangue and bio toxic residual coal. The rational utilization of coal gangue and treatment of residual coal pose significant environmental challenges. In this study, five strains with silicon activation capabilities were isolated from coal tailings, and a strain named \u003cem\u003eOchrobactrum sp.\u003c/em\u003e B2 was selected and purified. Using nitrosoguanidine (NTG) mutagenesis, a strain B2-M with stronger adaptability to coal tailings was obtained. Based on its metabolic characteristics, the conditions for biological leaching were optimized, resulting in an available silicon concentration in the leachate reaching 320.54 mg/L. Through direct and indirect leaching experiments, the adhesion and synergistic leaching mechanisms between bacteria and coal tailings were investigated. Kinetic results indicated that the biological leaching process follows chemical reaction control. FT-IR and GC-MS analyses demonstrated that the B2-M strain could disrupt and decompose the residual coal in coal tailings, converting it into utilizable small molecule organics and thus enhancing the leaching efficiency of available silicon from coal gangue. 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