Hypersynchronous iPSC-derivedSHANK2neuronal networks are rescued by mGluR5 agonism

ABSTRACT Variants in the gene encoding the postsynaptic scaffolding protein SHANK2 are associated with several neurodevelopmental disorders, including autism spectrum disorder. Here, we used in vitro multielectrode arrays and pharmacological manipulations to characterize how functional connectivity and network-level firing properties were altered in cultures of human iPSC-derived SHANK2 neurons. Using two isogenic pairs of SHANK2 cell lines, we showed that the SHANK2 hyperconnectivity phenotype was recapitulated at the network level. SHANK2 networks displayed significantly increased frequency and reduced duration of network burst events relative to controls. SHANK2 network activity was hypersynchronous, with increased functional correlation strength between recording channels. Analysis of intra-network burst firing dynamics revealed that spikes within SHANK2 network bursts were organized into high-frequency trains, producing a distinctive network burst shape. Calcium-dependent events called reverberating super bursts (RSBs) were observed in control networks but rarely occurred in SHANK2 networks. SHANK2 network hypersynchrony and numbers of strong correlations were fully rescued by the group 1 mGluR agonist DHPG, that also restored detection of RSBs and significantly improved network burst frequency and duration metrics. Our results demonstrate that SHANK2 variants produce a functional hyperconnectivity phenotype that deviates from the developmental trajectory of isogenic control networks. Furthermore, the hypersynchronous phenotype was rescued by pharmacologically regulating glutamatergic neurotransmission. Competing Interest Statement The authors have declared no competing interest.