Split Selectable Markers
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Abstract
Selectable markers are widely adopted in transgenesis and genome editing for selecting engineered cells with desired genotype but are limited in choices. We present here split selectable markers each allowing for selection of multiple “unlinked” transgenes in the context of lentivirus-mediated transgenesis as well as CRISPR/Cas-mediated biallelic knock-ins. Future development of split selectable markers may support enrichment or selection of “hyper-engineered” cells containing tens of transgenes or genetic modifications.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00