The frequency of bacterial pathogens of mastitis and their antibiotic susceptibility in Saanen and Alpine goats

Mastitis is mammary gland inflammation and as the purpose of Saanen and Alpine farms is milk production, control of mastitis is important. Antimicrobial resistance among mastitis pathogens has gradually been increasing. The aim of the present study was to isolate pathogenic bacteria from mastitis cases in Saanen and Alpine goats and to determine their antibiotic resistance in milk. The milk sample of 26 Saanen and 29 Alpine breeds with clinical mastitis was collected and cultured on general microbiological media. Then, the colonies were stained by gram staining and were assessed by differential media and biochemical tests. PCR was performed for the detection of Mycoplasma spp. The isolated bacteria were tested against 12 antibacterial disks. The significant difference in drug resistance levels between the Saanen and Alpine breeds was statistically assessed. Mycoplasma spp. was detected in 12.73% of samples. The frequency of the isolated bacteria was Escherichia coli (29.1%), Trueperella pyogenes (25.5%), Staphylococcus aureus (16.4%), Streptococcus agalactiae (9.1%), coagulase negative staphylococci (5.5%), and Corynebacterium pseudotuberculosis (1.8%) respectively. The significant difference between Saanen and Alpine was observed in antibiotic resistance to amoxicillin which showed more resistance in the Alpine breed. All the isolated bacteria showed multidrug resistance. Based on the obtained data using of antibiotics more accurate and using antibiogram test by clinician is necessary in the treatment of mastitis.