A Recommended Novel Green Economic Sample Preparation for Developed Verified Chromatographic Assays to Determine a Common Insecticidal Blend’s Residues on Samples of Natural Nutrition and Commercial Formulation for Governmental Guarantee in the Middle East

Abstract Spraying a tertiary blend of the insecticides; hexythiazox, imidacloprid, and thiamethoxam, on tomato fruits, are routine in agriculture-attentive countries, especially in the Middle East region, to protect them against insects’ attacks. Insecticide residuals represent one of the major environmental contamination and health risks. Specific HP-TLC and RP-HPLC methodologies are established to estimate the residual insecticides and subsequently unassuming sample collection procedures. In the planner chromatographic methodology, methanol: chloroform: glacial acetic acid: triethyl amine (8.5: 1.5: 0.2: 0.1, v/v) is recommended as a mobile system. The other one is columnar chromatography; acetonitrile: water (20: 80, v/v), pH 2.8, is recommended as a mobile system. The validation parameters are examined following the ICH rules. They are applied to field samples for estimating the insecticides’ residues, effectively.