Rare intra-tumoral colonization of Fusobacterium nucleatum in endometrial cancer

The presence of Fusobacterium nucleatum has been associated with gynecological disorders, namely endometriosis and pregnancy complications; however, its role in endometrial cancer remains unexplored. This study aimed to assess the presence of intra-tumoral F nucleatum in endometrial carcinoma tissues and its association with patients' clinicopathological and molecular features. A total of 260 patients were included, and a droplet digital polymerase chain reaction assay was employed to detect F nucleatum in formalin-fixed paraffin-embedded tumor tissues. F nucleatum was detected in only 3 cases (1.2%), and none exhibited high bacterial levels, even within the 79 microsatellite instability-high molecular subgroup. These findings suggest that intra-tumoral F nucleatum does not play an oncogenic role in endometrial cancer.