Rationalizing the efficiency of Cu-based MOF for the capture and biomimetic sequestration of Carbon dioxide

Abstract In light of their outstanding properties, metal-organic frameworks (MOFs) appear to be the latest and most assured sorbent in CCUS technology. In this study, adsorption isotherms of pure CO2, CH4, and N2, at three different temperatures were obtained for Cu-MOF and it showed a maximum CO2 adsorption uptake of 8.25 mmol g− 1 at 298 K and 30 bar. Cu-MOF was also employed as support to immobilize carbonic anhydrase (CA) derived from the bovine erythrocytes through cross-linked enzyme aggregates (CLEA). CO2 hydration as well as its transformation into CaCO3 was studied using the CA immobilized Cu-MOF (CA/Cu-MOF). The biocatalytic activity, thermal stability, reusability, and storage stability of CA/Cu-MOF were evaluated through para-nitrophenyl acetate (p-NPA) hydrolysis. CA immobilized on Cu-MOF exhibits a fairly low Kcat/Km value than the free CA; regardless of the above, the reusability of CA/Cu-MOF for about 20 cycles makes it a suitable biocatalyst of CO2 hydration under industrial operating conditions. The results demonstrated Cu-MOF as potential support for CA immobilization for CO2 sequestration and high CO2 uptake adsorbent.