Imaging Ultraweak Photon Emission from Living and Dead Mice and from Plants under Stress

The phenomenon of biological ultraweak photon emission (UPE), that is, extremely low-intensity emission (10 − 10 3 photons/cm 2 /sec) in the spectral range of 200 − 1000 nm, has been observed in all living systems that have been examined. Here we report experiments that exemplify the ability of novel imaging systems to detect variations in UPE for a set of physiologically important scenarios. We use EMCCD and CCD cameras to capture single visible-wavelength photons with low noise and quantum efficiencies higher than 90%. Our investigation reveals significant contrast between the UPE from live vs. dead mice. In plants we observed that an increase in temperature and injuries both caused an increase in UPE intensity. Moreover, chemical treatments modified the UPE emission characteristics of plants, particularly the application of an anesthetic (benzocaine) to injury, which showed the highest emission among the compounds tested. As a result, UPE imaging provides the possibility of non-invasive label-free imaging of vitality in animals and the responses of plants to stress.