Small molecules with hydantoin-containing and spiro-like structures promote devernalization in Arabidopsis

Small molecules with hydantoin-containing and spiro-like structures promote devernalization in Arabidopsis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Small molecules with hydantoin-containing and spiro-like structures promote devernalization in Arabidopsis Makoto Shirakawa, Nana Otsuka, Ryoya Yamaguchi, Hikaru Sawa, Naoya Kadofusa, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3780957/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 22 Jan, 2025 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Abstract Monocarpic plants flower only once and then produce seeds. Many monocarpic plants require a cold treatment known as vernalization before they flower. This requirement delays flowering until the plant is exposed to warm temperatures in the spring. Exposure to high temperature following vernalization causes devernalization, which cancels the vernalized state, resulting in delayed flowering and the resumption of vegetative growth. In this study, we screened over 16,000 chemical compounds and identified five small molecules (devernalizers; DVRs) that induce devernalization at room temperature without requiring a high-temperature treatment. Treatment with DVRs reactivated the expression of FLOWERING LOCUS C ( FLC ), a master repressor of flowering, and delayed flowering time. Three of the DVRs share two structures: a hydantoin-like region and a spiro-like carbon. Treatment with DVR06, which has a simple chemical structure that includes these structures, delayed flowering time, and reduced the amount of repressive histone modification at FLC . Our extensive chemical screening revealed that hydantoin and spiro are key chemical signatures that reduce repressive histone modifications and promote devernalization in plants. Biological sciences/Plant sciences/Plant physiology Biological sciences/Plant sciences/Plant development Full Text Additional Declarations There is NO Competing Interest. Supplementary Files 231220otsukaDVR06SupplemerntaryTable1.pdf Cite Share Download PDF Status: Published Journal Publication published 22 Jan, 2025 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3780957","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":266124435,"identity":"78d25780-09f1-4061-8f4f-45d04de4a649","order_by":0,"name":"Makoto 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