Optimization of xylanase production from Enterobacter asburiae PQ396173for hydrolysis of sugarcane bagasse

Optimization of xylanase production from Enterobacter asburiae PQ396173for hydrolysis of sugarcane bagasse | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Optimization of xylanase production from Enterobacter asburiae PQ396173for hydrolysis of sugarcane bagasse Arpita Sarangi, Hrudayanath Thatoi This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7226185/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 5 You are reading this latest preprint version Abstract This work aimed to isolate efficient microbial strains from Similipal Biosphere Reserve to produce xylanase enzyme and use it for the hydrolysis of sugarcane bagasse. Enzymatic assay for the xylanase activity was evidenced from the most potent bacterial strain SSXB1, and it was shown that in unoptimized conditions, SSXB1 showed the highest enzyme activity of 191.86 U/ml/min. In this work, the Box-Behnken design of response surface methodology was employed to optimize the culture conditions for the maximum production of xylanase from SSXB1. In an optimized condition i.e., temperature 35.0℃, pH 7.5, xylan conc. 0.15% and incubation time 36h, the enzyme activity was shown to reach 578.13 U/ml/min. The bacterial strain SSXB1 was identified as Enterobacter asburiae PQ396173 using biochemical and molecular methods. Further enzymatic hydrolysis of sodium carbonate pretreated sugarcane bagasse was optimized using E. asburiae PQ396173 xylanases that showed a good amount of reducing sugar liberation from pretreated biomass under optimized conditions. Additionally, scanning electron microscopy (SEM) analysis of untreated, sodium carbonate pretreated, commercial enzyme (BG-xylan) pretreated, and E. asburiae PQ396173 xylanase pretreated sugarcane bagasse revealed structural alterations in the pretreated biomass. This study is the first ever report about the production of xylanase from E. asburiae PQ396173. Xylanase Lignocellulosic biomass Bioethanol Xylanolytic bacteria Response surface methodology Scanning electron microscope (SEM) Full Text Cite Share Download PDF Status: Under Review Version 1 posted Reviewers agreed at journal 12 Aug, 2025 Reviewers invited by journal 12 Aug, 2025 Editor invited by journal 12 Aug, 2025 Editor assigned by journal 11 Aug, 2025 First submitted to journal 07 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7226185","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":499664435,"identity":"01858625-5e83-48a4-90f6-6b25f1563a8b","order_by":0,"name":"Arpita Sarangi","email":"","orcid":"","institution":"MSCB University: Maharaja Sriram Chandra Bhanja Deo University","correspondingAuthor":false,"prefix":"","firstName":"Arpita","middleName":"","lastName":"Sarangi","suffix":""},{"id":499664436,"identity":"7e712a7d-5d4d-48d4-b088-f5f7cea2dd9e","order_by":1,"name":"Hrudayanath Thatoi","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAxklEQVRIiWNgGAWjYBACAwYGNhDNww8iEwqI0sIM1JLAwCPZANJiQIIWBoMDUEsJAnPp/mMPfv6wkTE+vzrxwwMDBnl+sQP4tVjOOcxu2JOQxmN24+1mCaDDDGfOTiDgsBvJbBI8CYeBWs5uAGlJMLhNhBbJP0AtxjPObv5BtBZpkC0G/L3biLbFTFomLY1H4gbvNosEAwli/JL4TPKNjY09f//ZzTd/VNjI80sT0IIAEmCVEsQqBwH+A6SoHgWjYBSMgpEEAA/2QGMOozg4AAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-3368-1266","institution":"SOA SPS: Siksha O Anusandhan University School of Pharmaceutical Sciences","correspondingAuthor":true,"prefix":"","firstName":"Hrudayanath","middleName":"","lastName":"Thatoi","suffix":""}],"badges":[],"createdAt":"2025-07-27 12:37:29","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7226185/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7226185/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":89514166,"identity":"bdfae729-4951-46dd-9f14-b37b271e0815","added_by":"auto","created_at":"2025-08-20 19:14:43","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1223642,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript5.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7226185/v1_covered_ee030b55-1d51-4146-a887-2623d70b680e.pdf"}],"financialInterests":"","formattedTitle":"Optimization of xylanase production from Enterobacter asburiae PQ396173for hydrolysis of sugarcane bagasse","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"biologia","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"biol","sideBox":"Learn more about [Biologia](http://link.springer.com/journal/11756)","snPcode":"11756","submissionUrl":"https://www.editorialmanager.com/biol/default2.aspx","title":"Biologia","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Xylanase, Lignocellulosic biomass, Bioethanol, Xylanolytic bacteria, Response surface methodology, Scanning electron microscope (SEM)","lastPublishedDoi":"10.21203/rs.3.rs-7226185/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7226185/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis work aimed to isolate efficient microbial strains from Similipal Biosphere Reserve to produce xylanase enzyme and use it for the hydrolysis of sugarcane bagasse. Enzymatic assay for the xylanase activity was evidenced from the most potent bacterial strain SSXB1, and it was shown that in unoptimized conditions, SSXB1 showed the highest enzyme activity of 191.86 U/ml/min. In this work, the Box-Behnken design of response surface methodology was employed to optimize the culture conditions for the maximum production of xylanase from SSXB1. In an optimized condition i.e., temperature 35.0℃, pH 7.5, xylan conc. 0.15% and incubation time 36h, the enzyme activity was shown to reach 578.13 U/ml/min. The bacterial strain SSXB1 was identified as \u003cem\u003eEnterobacter asburiae\u003c/em\u003e PQ396173 using biochemical and molecular methods. Further enzymatic hydrolysis of sodium carbonate pretreated sugarcane bagasse was optimized using \u003cem\u003eE. asburiae\u003c/em\u003e PQ396173 xylanases that showed a good amount of reducing sugar liberation from pretreated biomass under optimized conditions. Additionally, scanning electron microscopy (SEM) analysis of untreated, sodium carbonate pretreated, commercial enzyme (BG-xylan) pretreated, and \u003cem\u003eE. asburiae\u003c/em\u003e PQ396173 xylanase pretreated sugarcane bagasse revealed structural alterations in the pretreated biomass. 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