Insights into multiple enzymes produced by an indigenously isolated novel Aeromonas veronii strain revealing diverse applications

In the quest for a novel microbe capable of producing multiple enzymes significant in industrial applications, bacterial isolates from soil samples collected from the wood-decaying area were screened. With this aim, the isolates were screened for the capability of producing various enzymes of industrial importance. Among the 100 screened isolates of bacteria, one strain identified as Aeromonas veronii by 16s RNA sequencing was found to be promising. It was revealed that the bacteria had the ability to produce cellulase, pectinase, protease, and amylases enzymes. The strain was set up for enzyme production and enzyme activity of cellulase, amylase, pectinase and protease were 0.071, 0.201, 0.202, 0.152 U/ml respectively. All four enzymes exhibited maximum activity at 40ºC with a distinct optimal pH. Since all the enzymes isolated from this strain are industrially significant, the application of the cell-free extract containing all these enzymes was evaluated at a laboratory scale that can be applied in industrial applications. The enzyme cocktail was found to be efficient in retting Crotalaria juncea stem fibers. The enzyme in combination with the detergent was effective in removing the blood egg yolk stain and show better extraction of fruit juices and oil. We have also reported that the enzyme extract could be used for sustainable waste management.