Polyethylenimine-based Magnetic Nanocomplexes Enhanced Cisplatin Toxicity on Ovarian Cancer Cells in Presence of Static Magnetic Field

Abstract BackgroundThe drug resistance of cancer cells is a major problem in the chemotherapy. Cisplatin (CIS) is one of the most effective chemotherapeutics used for ovarian cancer. Resistance to different chemo-drugs has developed over times. Here, we investigated the experimental approach to increase the CIS cytotoxicity and overcoming cell resistance through nanoparticle-based combination treatments. Polyethylenimine (PEI)-based magnetic iron oxide nanocomplexes were used to transfer the drugs in both genetically matched CIS-resistant (A2780CP) and -sensitive (A2780) ovarian cancer cells in presence of 20 mT static magnetic field. Magnetic nanoparticles (MNPs) were synthesized and bonded to PEI cationic polymer that leads to formation of binary complexes (PM). In the following, binding of CIS to PM binary complexes two including ternary complexes PM/C (PEI-MNP/CIS) and PMC (PEI-MNP-CIS) formed. ResultsOur results showed that the CIS cytotoxicity increased at different concentrations of CIS and PEI in all of binary and ternary treatments overtimes. Furthermore, CIS induced S and G2/M phase’s cell cycle arrest as well as reactive oxygen species (ROS) production in the both cell lines. Ternary complexes enhanced apoptosis more than binary complexes in the treated-cells. ConclusionsPEI-based magnetic noncomplex can be considered as a novel carrier for increasing the CIS cytotoxicity and probably overcoming drug resistance in ovarian cancer cells.