Study on the Mechanism of Lactobacillus Rhamnosus Probio-M9 on Staphylococcus Aureus Mastitis in Rat

Abstract This study aimed to investigate the potential anti-inflammatory mechanism of Lactobacillus rhamnosus Probio-M9 (Probio-M9), a probiotic strain previously isolated from the milk of healthy women. The Illumina Hiseq platform performed metagenomic sequencing on faeces samples at various time points. UPLC-Q-TOF/MS are used to detect a large number of endogenous metabolites in the serum of rats with mastitis induced by Staphylococcus aureus. The results showed that after oral Probio-M9, the beneficial intestinal bacteria (Lactobacillus murinus, Lactobacillus reuteri) were increasesed in intestinal bacteria of rats with mastitis, while the harmful bacteria(Enterococcus faecalis, Escherichia coli) were decreased. Moreover, it positively regulated the metabolites (linoleic acid, glycerophospholipid, aracidonic acid, sphinolipid, etc.) in the blood of rats with mastitis. This study provided a new research method for Probio-M9 to alleviate mastitis, and also provided a theoretical basis for the development and utilization of Probio-M9.