Effects of PPARγ agonist on the polarization of microglia around hematoma after intracerebral hemorrhage in male rats

Objective: To study the effect of PPARγ agonist (rosiglitazone) on the polarization of microglia around the hematoma after intracerebral hemorrhage in male SD rats. Materials and Methods: A total of 48 male SD rats were divided into a normal group (Control), the intracerebral hemorrhage model group (ICH), the sodium chloride intervention group (NaCl), and the rosiglitazone intervention group (RSG).ICH model of male rats was established by autologous blood. The neurological function of all groups of male rats was scored at 6, 24, 48, and 72h after modeling. The expression of CD16 (M1 marker) and CD206 (M2 marker) in the peripheral tissues of hematoma was detected by immunohistochemistry and Western blot. Results: Longa scores of the ICH and RSG group were significantly higher than those of the Control group and NaCl group at each time (P < 0.05). The RSG group's Longa score at 6, 24, and 48h was lower than that of the ICH group (P < 0.05). In the ICH group and RSG group, CD16 reached its peak at 6h and then decreased; the expression level of CD16 in the RSG group was lower than that in the ICH group at 6h (P < 0.05). The CD206 reached a peak at 24h; however, the expression level of CD206 in the RSG group was higher than in the ICH group (P < 0.05). Conclusion: PPARγ agonist stimulates the activation of M2 microglia , enhancing the ability to remove hematoma microglial cells.