× Pseudorhiza nieschalkii (Senghas) P.F.Hunt nothosubsp. siculorum H.Kertész & N.Anghelescu, 2020 A Intergeneric Orchid Hybrid New to Science

× Pseudorhiza nieschalkii (Senghas) P.F.Hunt nothosubsp. siculorum H.Kertész & N.Anghelescu, 2020 A Intergeneric Orchid Hybrid New to Science | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article × Pseudorhiza nieschalkii (Senghas) P.F.Hunt nothosubsp. siculorum H.Kertész & N.Anghelescu, 2020 A Intergeneric Orchid Hybrid New to Science Nora Anghelescu (aka Nora De Angelli), Hajnalka KERTÉSZ, Nicoleta Constantin, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-98364/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract We describe the first reported intergeneric hybrid to naturally occur between two subspecies belonging to different genera, Dactylorhiza fuchsii subsp. sooana (genus Dactylorhiza) and Pseudorchis albida subsp. tricuspis (genus Pseudorchis), as × Pseudorhiza nieschalkii (Senghas) P.F.Hunt nothosubsp. siculorum H.Kertész & N.Anghelescu, 2020. The hybrid was found and digitally photographed for the first time by Hajnalka Kertész in June, 2020, within Terra Siculorum, in one of the Natura 2000 protected areas, known as Harghita Mădăraș, ROSCI00090. Following detailed morphometric analysis using 67 characters and molecular karyological analyses, we identified this unique specimen as an intergeneric hybrid, new to science. The hybrid, an F1 generation plant, most likely representing a single intergeneric pollination event, is phenotypically intermediate between its parental species in most of the characters scored, but it significantly closely resembles Pseudorchis albida subsp. tricuspis parent. Since the parental species occurred in near proximity (1-10 meters distance), we suggest that the production of this hybrid required a minimum travel distance of ca 1-10 meters, by the pollinators and frequent exchange of pollen between the parental species was very likely. Plant Physiology and Morphology Terrestrial Ecology Forestry Pseudorhiza siculorum hybrid orchid Pseudorchis Dactylorhiza hybridization morphometry Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Full Text Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the latest manuscript can be downloaded and accessed as a PDF. Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-98364","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":4393184,"identity":"fa87671a-e559-495a-807f-7c8a401df6a0","order_by":0,"name":"Nora Anghelescu (aka Nora De Angelli)","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAUlEQVRIiWNgGAWjYLACHjDJ3HggweCfHIh54AFxWhgbDiQUHDAGa0kgWgvDhwOJDSA2Pi3m7L0PP7ypsMuTd29sOPDA4E76/LDDD4G22MnpNmDXYtlz3FhyzpnkYsMzB4EOM3iWu/F2mgFQS7Kx2QHsWgxupDFI87YxJ26ckQjSwpy7cXYCSMuBxG24tTD/5v1Xn7hx/kOwlnTD2ekfCGlhk+ZtOJw4XwIUYgaHE+SlcwjYcuYYm+WcY8cTN/CAHZZmuEE6pwDIwOOX423MN97UVCfObz988OGPPzby8rPTN3/4UGEnh0sLQu8BFIYBAeUgIN+AzhgFo2AUjIJRAAUAxMpunTvik0UAAAAASUVORK5CYII=","orcid":"","institution":"USAMV","correspondingAuthor":true,"prefix":"","firstName":"Nora","middleName":"Anghelescu (aka Nora","lastName":"De Angelli)","suffix":""},{"id":4393185,"identity":"cc0eb4bd-856b-400c-afea-891ef4d807ff","order_by":1,"name":"Hajnalka KERTÉSZ","email":"","orcid":"","institution":"Bethlen Gábor Middle School","correspondingAuthor":false,"prefix":"","firstName":"Hajnalka","middleName":"","lastName":"KERTÉSZ","suffix":""},{"id":4393186,"identity":"23131868-d54e-4f07-aef6-96142f8f4195","order_by":2,"name":"Nicoleta Constantin","email":"","orcid":"","institution":"University of Bucharest","correspondingAuthor":false,"prefix":"","firstName":"Nicoleta","middleName":"","lastName":"Constantin","suffix":""},{"id":4393187,"identity":"448eef60-2e4f-4f35-87e9-9fbab8701ad9","order_by":3,"name":"Alexandra SIMON-GRUIȚA","email":"","orcid":"","institution":"University of Bucharest","correspondingAuthor":false,"prefix":"","firstName":"Alexandra","middleName":"","lastName":"SIMON-GRUIȚA","suffix":""},{"id":4393188,"identity":"5343220b-7b3e-484d-bf16-e305c4c0d51c","order_by":4,"name":"Gerogiana DUȚĂ CORNESCU","email":"","orcid":"","institution":"University of Bucharest","correspondingAuthor":false,"prefix":"","firstName":"Gerogiana","middleName":"DUȚĂ","lastName":"CORNESCU","suffix":""},{"id":4393189,"identity":"ad4e2fad-4d74-49bc-86b5-e0543a32ff41","order_by":5,"name":"Maria D. POJOGA","email":"","orcid":"","institution":"University of Bucharest","correspondingAuthor":false,"prefix":"","firstName":"Maria","middleName":"D.","lastName":"POJOGA","suffix":""},{"id":4393190,"identity":"76e21054-bad9-4dd0-81f2-018fced5fafb","order_by":6,"name":"Mihaela Georgescu","email":"","orcid":"","institution":"USAMV","correspondingAuthor":false,"prefix":"","firstName":"Mihaela","middleName":"","lastName":"Georgescu","suffix":""},{"id":4393191,"identity":"5991068d-8281-4258-8eb2-6aaf6ba9aae7","order_by":7,"name":"Sorina Petra","email":"","orcid":"","institution":"USAMV","correspondingAuthor":false,"prefix":"","firstName":"Sorina","middleName":"","lastName":"Petra","suffix":""},{"id":4393192,"identity":"0f6494ec-820d-473e-b2e0-e39dd6ca0795","order_by":8,"name":"Florin Toma","email":"","orcid":"","institution":"USAMV","correspondingAuthor":false,"prefix":"","firstName":"Florin","middleName":"","lastName":"Toma","suffix":""}],"badges":[],"createdAt":"2020-10-26 13:41:38","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-98364/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-98364/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":3471684,"identity":"98eafe98-96ad-491f-9bc4-3f3a84316045","added_by":"auto","created_at":"2020-11-09 19:38:39","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":1454498,"visible":true,"origin":"","legend":"The map of the studied area within Harghita county, Harghita Mountains and the protected areas ROSCI00090 Harghita – Mădăraș and ROSPA0034 Depresiunea și Munții Ciucului (map used by permission of National Agency for Protected Areas Harghita Territorial Service) ","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/1c249fbafcb391f6a9d9ba0d.png"},{"id":3471685,"identity":"e3da0816-cfe1-407a-bba8-3cb0a3bf4281","added_by":"auto","created_at":"2020-11-09 19:38:39","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":1048085,"visible":true,"origin":"","legend":" Dactylorhiza fuchsii (Druce) Soó subsp. sooana (Borsos) Borsos entire plant in its natural habitat (A), details of the inflorescences (B, C) \nOne of the inflorescences are visited by hoverfly of the family Syrphidae Latreille, 1802. The hoverflies, which are true bee-mimics, are very frequent visitors of D. fuschii subsp. sooana, although it has not been shown to be its real pollinators, as of yet. Photos A-C © N. Anghelescu originals ","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/ae4ba984de1714dc804b1dd8.png"},{"id":3471686,"identity":"346da61f-85cc-4821-8fc2-b3d94bfdc1d0","added_by":"auto","created_at":"2020-11-09 19:38:39","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":2103887,"visible":true,"origin":"","legend":"Pseudorchis albida (L.) Á.Löve \u0026 D.Löve subsp. tricuspis (Beck) E.Klein entire plant in its natural habitat (A), details of the inflorescences (B, C) \nOne of the inflorescences are visited by hoverfly of the family Syrphidae Latreille, 1802. The hoverflies, which are true bee-mimics, are also very frequent visitors of Pseudorchis albida subsp. tricuspis, although it has not been shown to be its real pollinators, as of yet. Photos A-C © N. Anghelescu originals ","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/da9a59c17b7b8d1f80a78d1e.png"},{"id":3471687,"identity":"a7e782dd-9f0a-4bcd-9356-8e1e86bc7388","added_by":"auto","created_at":"2020-11-09 19:38:39","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":258910,"visible":true,"origin":"","legend":"This is the first image of × Pseudorhiza nieschalkii nothosubsp. siculorum ever taken by H. Kertész at 08:39:19am, on 30th of June, 2020. While researching the existing orchid species of the heathland, she spotted an oddly looking plant, which, from a distance, resembled P. albida subsp. tricuspis. She approached the plant and, after parting the dense grass that surrounded it, she realised it was something more than a simple P. albida subsp. tricuspis. Minutes later digital images were sent to N. Anghelescu who confirmed the putative parents and, in that regards, suggested that the hybrid may actually be new to science. Photo © H. Kertész original ","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/8fc8cbf221daa80be0ffdabe.png"},{"id":3471688,"identity":"87041799-4838-4a3d-9408-233b6930fd3e","added_by":"auto","created_at":"2020-11-09 19:38:39","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":985075,"visible":true,"origin":"","legend":"Morphological comparisons of × Pseudorhiza nieschalkii nothosubsp. siculorum and parents (A-D). The following three comparison diagrams show the intermediate characters of the hybrid’s habitus (A), inflorescence (B) and flowers (C), compared to those of its parents. The hybrids are almost as tall as the parents. The hybrid’s inflorescence is less floriferous and flat topped, resembling more that of D. fuchsii subsp. sooana parent. The flowers’ labellum is deeply three-lobed, very similar to P. albida subsp. tricuspis’ labella. Photos A-C © N. Anghelescu originals ","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/387e3cb3c9113d1fd3d8cfd2.png"},{"id":3471689,"identity":"058c2a29-c513-4602-bec5-13d2e6a33d78","added_by":"auto","created_at":"2020-11-09 19:38:40","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":1249146,"visible":true,"origin":"","legend":"Chromosomes at somatic metaphase (Feulgen staining) of × Pseudorhiza nieschalkii nothosubsp. siculorum hybrid 2n = 40 (±2), no. 28-2; no. 30-1; no. 31-4. Scale bar = 1 μm. ","description":"","filename":"6.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/8c4fa23b5f352bb76215c12f.png"},{"id":3471690,"identity":"00aa3844-2b17-4f93-be18-258b426c32a0","added_by":"auto","created_at":"2020-11-09 19:38:40","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":652170,"visible":true,"origin":"","legend":"The distribution map of × Pseudorhiza nieschalkii nothosubsp. siculorum in Harghita – Mădăraș protected area that yields the holotype and only known plant (map used by permission of National Agency for Protected Areas Harghita Territorial Service) ","description":"","filename":"7.png","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1/af69ea2c6bc3837f4ae4f1f2.png"},{"id":13553612,"identity":"1b7f593c-2def-40f2-a564-58a8cfa02bc5","added_by":"auto","created_at":"2021-09-17 02:37:49","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1566833,"visible":true,"origin":"","legend":"","description":"","filename":"NoraAnghelescuXXPseudorhizanieschalkiinothosubsp.siculorumH.KerteXXszXN.AnghelescuNature.pdf","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1_covered.pdf"},{"id":3471691,"identity":"c6578a21-a8d6-4790-9967-d402d894c99c","added_by":"auto","created_at":"2020-11-09 19:38:43","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":6626796,"visible":true,"origin":"","legend":"","description":"","filename":"NoraAnghelescuXXPseudorhizanieschalkiinothosubsp.siculorumH.KerteXXszXN.AnghelescuNature.pdf","url":"https://assets-eu.researchsquare.com/files/rs-98364/v1_stamped.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"\u003cp\u003e× Pseudorhiza nieschalkii (Senghas) P.F.Hunt nothosubsp. siculorum H.Kertész \u0026amp; N.Anghelescu, 2020 A Intergeneric Orchid Hybrid New to Science\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. 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The hybrid was found and digitally photographed for the first time by Hajnalka Kertész in June, 2020, within Terra Siculorum, in one of the Natura 2000 protected areas, known as Harghita Mădăraș, ROSCI00090. Following detailed morphometric analysis using 67 characters and molecular karyological analyses, we identified this unique specimen as an intergeneric hybrid, new to science. The hybrid, an F1 generation plant, most likely representing a single intergeneric pollination event, is phenotypically intermediate between its parental species in most of the characters scored, but it significantly closely resembles Pseudorchis albida subsp. tricuspis parent. 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