Highly Efficient Coreactant-Free Electrochemiluminescence Sensing Platform Using Novel Microfabricated Multiplexed Entwined Spiral Microelectrodes for Point-of-Care Applications

Highly Efficient Coreactant-Free Electrochemiluminescence Sensing Platform Using Novel Microfabricated Multiplexed Entwined Spiral Microelectrodes for Point-of-Care Applications | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 6 November 2025 V1 Latest version Share on Highly Efficient Coreactant-Free Electrochemiluminescence Sensing Platform Using Novel Microfabricated Multiplexed Entwined Spiral Microelectrodes for Point-of-Care Applications Authors : Somayyeh Bozorgzadeh 0000-0002-0162-0573 [email protected] , Richard Murray , Hassan Hamidi , Ian Seymour , Daniela Iacopino , and Alan O'Riordan Authors Info & Affiliations https://doi.org/10.22541/au.176241476.68170275/v1 145 views 72 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Luminol-based Electrochemiluminescence (ECL) generates weak signals in neutral media and typically requires H 2 O 2 as a coreactant. However, H 2 O 2 instability and the need for on-site addition hinder real-time diagnostic applications. Compact integrated sensing platforms are ideal for point-of-care (POC) testing due to portability, low sample requirements, and multiplexing. However, as sensor dimensions decrease, the low light emission issue in ECL becomes severe. We introduce a novel fully integrated miniaturized silicon device consisting of three sensors comprised of unique entwined micro-spiral electrodes in a generator-collector configuration. This enables highly sensitive, coreactant-free multiplexed sensing at physiological pH by accelerating in-situ reactive oxygen species production and boosting ECL intensity. Systematic optimization of electrode geometry (gaps and widths) yields an 11-fold improvement in ECL signal compared to a single-electrode setup, as well as excellent reproducibility and stability. In addition to Trolox and H 2 O 2 detection, the platform demonstrates multiplex immunosensing through selective functionalization of the collector electrodes with chitosan nanocomposites, followed by Protein A/G-assisted immobilization of anti-IgG antibodies with peptide-based antifouling. The immunosensors exhibit high analytical performance (linear range: 0.001-100 pg·mL -1 , detection limit: 0.8 fg·mL -1 ) with excellent reproducibility and reliability in 25% fetal bovine serum, highlighting the platform’s potential for sensitive, coreactant-free multi-analyte POC diagnostics. Supplementary Material File (manuscript.docx) Download 25.86 MB Information & Authors Information Version history V1 Version 1 06 November 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords coreactant-free electrochemiluminescence entwined spiral microelectrodes multiplexed platform Authors Affiliations Somayyeh Bozorgzadeh 0000-0002-0162-0573 [email protected] Tyndall National Institute View all articles by this author Richard Murray Tyndall National Institute View all articles by this author Hassan Hamidi Tyndall National Institute View all articles by this author Ian Seymour Tyndall National Institute View all articles by this author Daniela Iacopino Tyndall National Institute View all articles by this author Alan O'Riordan Tyndall National Institute View all articles by this author Metrics & Citations Metrics Article Usage 145 views 72 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Somayyeh Bozorgzadeh, Richard Murray, Hassan Hamidi, et al. Highly Efficient Coreactant-Free Electrochemiluminescence Sensing Platform Using Novel Microfabricated Multiplexed Entwined Spiral Microelectrodes for Point-of-Care Applications. Authorea . 06 November 2025. DOI: https://doi.org/10.22541/au.176241476.68170275/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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