Aquaporin Expression in Endometriosis Infertile Mice and Intervention Effect of Eleng Capsule

Objective: To investigate the aquaporin(APQ)expression in endometriosis infertile mice and the intervention effect of Eleng capsule. Methods:The mice were divided into four groups,namely normal group,model group,Danazol group and Eleng group. The normal group and the model group were administrated with distilled water,0.25 mL for per 10 g of body weight,and Danazol group was administrated with Danazol,0.4 g for per kilogram of body weight,while Eleng group was administrated with Eleng capsule,4 g for per kilogram of body weight. The treatment course lasted 30 days. The serum estradiol level was measured by direct chemiluminescence method,and the mRNA expression of aquaporins in the endometrial tissue was measured by RT-PCR technique. Results:The level of serum estradiol in the model group was higher than that in the normal group(P0.05). Serum estradiol level in both Danazol group and Eleng group was close to that in the normal group(P0.05). AQP1 expression level in model group,Danazol group and Eleng group was similar to that in the normal group(P0.05),while AQP2 expression level was decreased, the inter-group difference being insignificant(P 0.05).Conclusion: Endometriosis infertile mice have higher level of serum estradiol,and Danazol group and Eleng capsule have counteraction on the estradiol level. Further research is still required to explore the APQ expression and the intervention effect of Eleng capsule.