Effects of a one-week vacation with various activity programs on well-being, heart rate variability, and sleep quality in healthy vacationers - an open comparative study

Effects of a one-week vacation with various activity programs on well-being, heart rate variability, and sleep quality in healthy vacationers - an open comparative study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effects of a one-week vacation with various activity programs on well-being, heart rate variability, and sleep quality in healthy vacationers - an open comparative study Markus Hübner, Peter Lechleitner, Günther Neumayr This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1946353/v2 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 27 Dec, 2022 Read the published version in BMC Public Health → Version 2 posted 8 You are reading this latest preprint version Show more versions Abstract Objectives: This open comparative study aimed to analyze the effects of a one-week vacation with various activity programs on well-being, heart rate variability (HRV) and sleep quality in healthy vacationers. Methods: 52 healthy untrained vacationers spent a one-week vacation with regular exercise in East Tyrol. Exercise was performed on six of seven days. The study participants were divided into a) Group 1, playing golf (G), and b) Group 2 performing Nordic walking or e-biking (NW&EB). Well-being was measured with the WHO-5 well-being-index; stress and recovery status was obtained with the EBF-24-questionnaire (recovery-stress questionnaire). HRV-parameters were measured with a 24-hour-ECG (electrocardiogram). Sleep quality was derived from the EBF-24 questionnaire and sleep architecture from HRV-analysis. Examinations were performed one day before and after the vacation. Results: Well-being significantly improved in the G group (+40%, p < 0.001) and NW&EB group (+19%, p = 0.019). The stress and recovery profile also improved significantly in both groups (stress-decrease: -43.7% G group; -44.7% NW&EB group; recovery-increase: +23.6% G group; +21.5% NW&EB group). Except for the SDNN (standard deviation of the NN interval), no significant change was noted in HRV-parameters. SDNN improved significantly only in the NW&EB group (+9%, p < 0.05). Sleep quality (+21% G group, p = 0.029; +19% NW&EB group, p = 0.007) and architecture (-10% G group, p = 0.034; -23% NW&EB group, p = 0.012) significantly improved in both groups. Conclusion: A short-term vacation with regular exercise was well tolerated by the study participants and improved well-being, sleep quality, HRV and autonomic regulation. Trial registration: Registry and the registration no. of the study/trial: Approval was received from the ethics committee of the Leopold Franzens University of Innsbruck (AN2013-0059 332/4.8) physical activity vacation psychological well-being stress heart rate variability sleep quality Figures Figure 1 Figure 2 1. Introduction Regular physical training has positive effects on both physical and mental health, supports the individual to achieve an ongoing reduction of daily stress, and is recommended worldwide as a beneficial lifestyle measure by various health organization [ 1 – 3 ]. Vacation, defined by Lounsbury and Hoopes in 1986 as a temporary period of several days or weeks off from work, is also considered “healthy” for recovery from work stress, although scientific data in this regard are scarce. Scientific research investigating the impact of vacation and exercise therein on medical risk factors or established health parameters is virtually non-existent [ 4 – 6 ]. In Western societies holiday behavior reveals a tendency towards short vacations. The question arises as to whether short-term vacations provide sufficient recovery from work and everyday stress [ 7 ]. A chronic unstable balance between stress and recovery will cause exhaustion sooner or later; which cannot be compensated by a weekend or a few days off from work [ 8 ]. Any chronic overload situation, regardless of whether it is a burnout syndrome at the emotional-psychological level or an overtraining syndrome at the physical level, causes an alteration of HRV parameters [ 9 ]. Physical training, defined by specific criteria such as regularity, duration, and the intensity of exercise above an individual threshold causes physiological adaptations in all organ systems, including autonomic regulation of the cardiac system [ 10 ]. Therefore, HRV analysis is probably the most important non-invasive method to study changes in various autonomic functions [ 11 ]. The study design of the East Tyrol Health Tourism Study (OGTS) was such that all sports activities – golf, Nordic walking and e-biking – were of low to moderate exercise intensities and could be performed by nearly everyone, including untrained persons. The parameters investigated in this part of the OGTS, i.e. psychological well-being, HRV, and sleep quality are influenced negatively by any kind of stress and positively by the quantity of regeneration and physical activity. The purpose of the OGTS was to investigate the cumulative health effects of a one-week vacation and regular physical activity with regard to three hypotheses: Hypothesis 1 (H1): A short-term vacation with moderately intensive sports activities has measurable and positive effects on psychological well-being. Hypothesis 2 (H2): HRV is positively influenced by a week of active vacation. Hypothesis 3 (H3): The quality of sleep is improved during a vacation. 2. Materials And Methods 2.1. Design and procedure The present study was part of the East Tyrol Health Tourism Study (OGTS) and investigated the effects of a short-term vacation with activity programs on psychological well-being, and various autonomic functions such as HRV and sleep quality in healthy volunteers. The cardiovascular and metabolic findings of the OGTS - describing the effects on performance capacity, blood pressure, heart rate and diastolic heart function as well as changes in metabolism and the adipokine profile - were published previously [ 4 , 6 ]. In an open comparative study, 52 healthy untrained vacationers spent a one-week active vacation in East Tyrol. All participants provided their written consent prior to the study [ 4 ]. The study was approved by the ethics committee of the Medical University of Innsbruck. The study participants were accommodated at one of the eight hotels in East Tyrol, at an altitude of 670 to 980 meters above sea level [ 4 ]. Three sports programs were available: golf, Nordic walking, and e-biking. Nordic walking and e-biking were combined in a single group because the exercise intensity and duration were similar. Group 1 played golf and group 2 performed Nordic walking or e-biking (NW&EB). Group assignment was based on the vacationer‘s preference. The mean duration of exercise during an e-biking or walking tour was 2.4 hours in the NW&EB group. The mean duration of exercise was 33.5 h/week in the G group and 14.2 h/week in the NW&EB group. Every training unit was guided by a fitness instructor. The participants’ daily caloric intake was not taken into account; a specific diet plan was not followed. Psychological well-being was registered on the WHO-5 well-being index, and the current stress and recovery profile with the EBF-24 questionnaire on stress and recovery. HRV analysis was performed on the basis of a 24-hour-ECG. Sleep quality was registered on the EBF-24 questionnaire, and sleep architecture was derived from the HRV analysis. All examinations were performed one day before and after the vacation. A second survey of the OGTS was carried out two years after the one-week vacation to examine long-term effects in terms of lifestyle modification, diet, physical activity, and the ability to relax. The protocol timeline is provided in ( Table 1 ) . 2.2. Measurements 2.2.1. Well-being Psychological well-being was assessed with the German version of the original WHO-5 well-being index of 1998. The WHO-5 questionnaire includes positively phrased feelings such as a good mood, feeling calm and relaxed, active and vigorous, fresh and rested, and interested in matters of everyday life [ 12 ]. Results are expressed on a 6-point Likert scale for each item, ranging from 0 to 5: 0 = at no time, 1 = some of the time, 2 = less than half the time, 3 = more than half the time, 4 = most of the time, 5 = all of the time. This provides a prime score ranging from 0 to 25. The result is multiplied by 4 to obtain a standardized percentage of points, yielding a figure between 0% and 100% [ 12 ]. 2.2.2. Stress and recovery The recovery-stress questionnaire (EBF) used in the present study was the German version of the EBF-Basic-24-A3 / B3. The EBF-24 questionnaire comprises 12 topics: 7 of these address stress and 5 address recovery. Each topic consists of 2 items [ 13 , 14 ]. We evaluated 4 of 7 topics addressing stress: feelings such as dejection, irritability, tiredness and subjective malaise, and 4 of 5 subjects addressing recovery: feelings such as relaxation, mood, satisfaction, and sleep quality. Each of the 12 subjects consisting of 2 items was mapped on a 7-point Likert scale ranging from 0 (never) to 6 (always). The questionnaire was modified to the current study design, and assessed the previous 7–14 days and nights [ 13 , 14 ]. 2.2.3. HRV HRV analysis was performed with a 24-hour-ECG (Medilog AR12 plus®, Schiller, Feldkirchen, Germany). The following parameters were measured: SDNN, r-MSSD, pNN50, total power and log LF/HF. SDNN: standard deviation of the NN intervals in ms; pNN50: percentage of the intervals with at least 50 ms deviation from the previous interval; r-MSSD: root mean square of successive differences between normal heartbeats; log LF/HF: quotient of the logarithm of low frequency to high frequency; total power: total number of all frequency ranges [ 15 , 16 ]. 2.2.4. Sleep quality Sleep quality was investigated by the EBF-24 questionnaire, the apnea index, and HRV analysis. The following parameters were used to assess sleep architecture from HRV analysis: nocturnal, tub shaped heart rate depression, respiratory sinus arrhythmia (ranging from 0.2 to 0.3 Hz), arousals, and periods of deep sleep [ 3 , 17 – 19 ]. Based on these parameters and on HRV measurements, we determined a score ranging from 1 to 4 for each study participant (1 = normal sleep architecture; 4 = strongly disturbed sleep architecture). 2.3. Statistical analysis IBM SPSS Statistics for Mactonish, Version 26.0 (IBM Corp., Armonk, N.Y., USA) was used for statistical analysis. Wilcoxon’s rank sum test was employed to determine p-values for the before-and-after comparison. The interquartile range (IQR, 25th and 75th percentiles) was also calculated. The level of statistical significance was set to p < 0.05. Table 1 Spirit (standard protocol items: recommendations for interventional trials) - protocol timeline Enrolment Allocation Post allocation TIMEPOINT -t 1 0 t 1 (1 day before vacation) t 2 (1-week vacation) t 3 (1 day after vacation) t 3 − 4 (up to 2 years after vacation) ENROLMENT : Eligibility screen X Informed consent X Allocation X INTERVENTIONS : [Golf] [Nordic walking or e-biking] ASSESSMENTS : [psychological well-being] X X [HRV measurement] X X [Sleep architecture] X X [second survey] X 3. Results Fifty-two persons participated in the study (30 men and 22 women). Their median age was 54.3 years. Baseline characteristics were similar in both groups. The sole significant difference was the mean exercise duration secondary to the study design. (Table 2 ) summarizes the baseline characteristics of the study participants. Table 2 Baseline characteristics of the study participants before the one-week vacation Parameter Golf group NW&EB group Number (n) 30 22 Age (years) 54 (47–63) 54 (47–60) Gender (m, %) 53 64 BMI (kg/m²) 27 (23–30) 27 (22–30) Mean exercise duration/week (h) 33.5 14.2 Results shown as medians and interquartile range (IQR). 3.1. WHO-5 well-being index Before the vacation, the mean score on the WHO-5 well-being index was 15.3 in the G group and 16.45 in the NW&EB group. After the vacation, an increase of 5.29 points was noted in the G group and 3.14 points in the NW&EB group. The WHO-5 well-being index increased to 20.59 in the G group and 19.59 in the NW&EB group. The standardized percentage values increased from 60–84% in the G group (p < 0.001), and from 64–80% in the NW&EB group (p = 0.019). This corresponds to a relative percentage increase of 40% in the G group and 19% in the NW&EB group. The increase in the WHO-5 well-being index observed in both groups implies a significant improvement of well-being after one week of active vacation. 3.2. EBF-24 The questionnaire was divided into two parts: stress and recovery. A detailed group comparison of mean values is given in ( Table 3 ) . 3.2.1. Stress Perceived stress was reduced significantly after one week in both groups. The NW&EB group showed a significant point reduction in all four subtests evaluated for stress – dejection , irritability , tiredness and subjective malaise – which corresponds to a reduction of stress. The group participants felt less dejected (-50%, p = 0.044 ), less irritable (-52%, p = 0.05 ), less tired (-38%, p = 0.045 ) and less uncomfortable (-42%, p = 0.006 ). Golfers, by comparison, achieved statistical significance in 3 of 4 subtests: dejection (-65%, p = 0.003 ), irritability (-38%, p = 0.004 ) and tiredness (-52%, p = 0.08 ). No significant outcome was observed for subjective malaise (-20%, p = 0.874 ). In a group comparison, the improvement in feelings such as dejection , irritability and tiredness were more pronounced in the G group than in the NW&EB group. The cumulative stress value, which comprised mean values for dejection, irritability, tiredness and subjective malaise , decreased by 6 points in the G group (from 13.73 to 7.73 points), corresponding to a percentage decrease of 43.7%. In the NW&EB group, this value decreased by 5.3 points (from 11.9 to 6.6 points) or 44.7%. This resulted in a decrease of ~ 44% for stress reduction in the entire group (G + NW&EB). 3.2.2. Recovery In both groups, the recovery profile improved after one week. The increase in all four evaluated subtests was more significant in golfers. Group members felt more satisfied , happier , more relaxed , and more rested than they did before the holiday. The score achieved by golfers on the EBF-24 questionnaire for satisfaction increased from 7.53 to 8.97 points [relative percentage increase of 19% ( p = 0.003 )]. The NW&EB group showed a measurable but non-significant increase of 11% in satisfaction ( p = 0.063 ). In both groups, the study participants felt more relaxed after their vacation. The average increase in relaxation was 32% in the G group and 35% in the NW&EB group ( p < 0.005 ). Improvements in happiness (+ 25%, p = 0.007 ), relaxation (+ 35%, p = 0.001 ) and sleep quality (+ 19%, p = 0.007 ) were more pronounced in the NW&EB group than in the G group. The latter achieved significant increases in their scores for happiness (+ 24%, p = 0.021 ), relaxation (+ 32%, p = 0.002 ) and sleep quality (+ 21%, p = 0.029 ). The cumulative recovery value, which comprised the mean values for satisfaction , happiness , relaxation and sleep quality , resulted in a point increase of 6.7 (from 28.4 to 35.1 points) in the G group, which corresponds to a percentage increase of 23.6%. This value increased by 5.8 points (from 27 to 32.8 points) or 21.5% in the NW&EB group. The entire group (G + NW&EB) experienced an improvement of ~ 23% in regeneration and recovery. 3.3. HRV There was a small but non-significant change in all measured HRV parameters, except for a significant improvement of SDNN in the NW&EB group. The changes were similar in both groups. r-MSSD, pNN50, log LF/HF and total power improved slightly but non-significantly in terms of increased parasympathetic activity in both groups. The mean SDNN was high at the start of the vacation in the NW&EB group, and increased significantly from 45.67 ms to 49.67 ms, corresponding to a relative percentage increase of 9% ( p < 0.05 ). Details of the HRV parameters are shown in ( Table 3 ) . 3.4. Sleep quality based on the EBF-24 questionnaire, HRV analysis, and apnea index 3.4.1. Sleep architecture (HRV) The score of the G group for sleep architecture was 2.44 (on a scale from 1 to 4) before the vacation, and decreased to 2.2 (-10%, p = 0.034 ) after the vacation. This result signifies an improvement from a moderately to a slightly disturbed sleep architecture. The decrease was even more pronounced in the NW&EB group. The mean value in this group decreased from 2.35 to 1.8 (-23%, p = 0.012 ). Sleep architecture changed significantly; it improved from a moderately to a slightly disturbed sleep architecture in the G group, and from a slightly disturbed to a nearly normal sleep architecture in the NW&EB group. A clear increase in nocturnal parasympathetic activity was noted on the HRV spectrogram. (Fig. 1) and (Fig. 2) show changes in sleep architecture throughout the vacation in one study participant. 3.4.2. Sleep quality EBF-24 All study participants felt more rested in the morning and less tired after one week of active vacation compared to the beginning. The number of points on the EBF-24 questionnaire increased from 7.58 to 9.13 after the vacation in the G group ( p = 0.029 ), and from 7.55 to 8.95 in the NW&EB ( p = 0.007 ). The improvement of sleep quality was significant in both groups, but more pronounced in the NW&EB group. 3.4.3. Apnea index The apnea index was calculated on the basis of the HRV analysis, and revealed no significant change in either group. The number of nocturnal apneas was only reduced among golfers (from 6.66 to 5.32). However, the apnea index increased from 7.64 to 7.84 in the NW&EB group. Specific data on sleep quality are summarized in ( Table 3 ) . HRV spectrogram of a study participant one day before the vacation. The red ellipsoidal marking indicates a low frequency density in the HF spectrum (0.15-0.4 Hz), whereas the weak yellow coloring indicates reduced nocturnal PNS activity. X-axis: time of the HRV analysis; y-axis: frequency spectrum in Hz; Hz: Hertz; HF: high frequency. This spectrogram shows poor sleep quality and diminished nocturnal parasympathetic activity. The red ellipsoidal marking highlights the increased frequency density in the HF spectrum (0.15-0.4 Hz). Increasing yellow and light red coloring express good nocturnal parasympathetic activity. X-axis: time of the HRV analysis; y-axis: frequency spectrum in Hz; Hz: Hertz; HF: high frequency. This spectrogram shows improved sleep quality with demonstrable nocturnal parasympathetic activity. Table 3 Heart rate variability, well-being and sleep quality in the golf and Nordic walking / e-biking groups before and after the vacation. Parameter Golf group (n = 30) NW&EB group (n = 22) Before the vacation After the vacation Before the vacation After the vacation EBF-24 stress Dejection Irritability Tiredness Subjective malaise 2.89 (1–4) 3.21 (2–4) 4.32 (2.5–5) 3.32 (1.5–4) 1 (0–1.75) *** 2 (1–2) *** 2.07 (0.25–3) ** 2.67 (1.25–4) 1.91 (0–3) 3.23 (2–4.75) 3.27 (2–4.75) 3.50 (2–5) 0.95 (0–1) * 1.55 (0–2) ** 2.05 (1–3) * 2.05 (1.25–2) ** EBF-24 recovery Happiness Relaxation Satisfaction Sleep quality 6.37 (5.5–8) 6.89 (5.5–8) 7.53 (6–9.5) 7.58 (7–9) 7.87 (6.25–9) * 9.10 (8–10) *** 8.97 (8–10) *** 9.13 (8.25–11) * 5.32 (3–8) 6.41 (4–8) 7.77 (6.25–9.75) 7.55 (6–10) 6.64 (5–8.75) ** 8.68 (8–10) *** 8.59 (8–10) 8.95 (8–11) ** WHO-5 15.3 (12–18) 20.59 (18.25–22) **** 16.45 (13.5–20) 19.59 (17.25–21.75) * SDNN (ms) 41.74 (32.05–48.85) 40.88 (34.7–46.4) 45.67 (36–53) 49.67 (40–56) * pNN50 (%) 1.87 (0.5–2.5) 3.23 (0.57–3.6) 2.74 (0.77–2.9) 3.67 (0.71–5.54) r-MSSD (ms) 20.09 (12.6–19.6) 20.91 (12.8–24.3) 19.51 (16–21.4) 21.84 (17.7–26.7) log LF/HF (%) 0.68 (0.59–0.82) 0.67 (0.54–0.79) 0.61 (0.5–0.7) 0.60 (0.44–0.73) Total power (ms2) 2021 (1224–2619) 2375 (1341–2979) 3212 (2415–4111) 3555 (2468–4714) Sleep architecture 2.44 (2–3) 2.20 (2–3) * 2.35 (2–3) 1.80 (1–2) * Sleep quality (EBF-24) 7.58 (7–9) 8.68 (7–9) 7.55 (6–10) 8.95 (8–11) Apnea index 7.04 (0.71–10.6) 5.32 (0.5–9.12) 7.64 (3–8) 7.84 (4.6–10.3) Results shown as mean values rounded to two decimal places and interquartile range (IQR) in the golf vs. NW&EB groups. NW&EB: Nordic walking and e-biking; WHO-5: World Health Organization-5 index of well-being; SDNN: standard deviation of the NN intervals in ms; pNN50: percentage of the intervals with at least 50 ms deviation from the previous interval; r-MSSD: root mean square of successive differences between normal heartbeats; log LF/HF: quotient of the logarithm of low frequency to high frequency; total power: total number of all frequency ranges; Sleep architecture (HRV): evaluation of sleep quality based on heart rate variability; Sleep quality (EBF-24): evaluation of sleep quality based on the stress-recovery questionnaire; Apnea index: number of cessations of breathing; ms: milliseconds; ms²: milliseconds squared. p-value within the group: vs . before the vacation * p < 0.05 ; vs . before the vacation ** p < 0.01 ; vs . before the vacation *** p < 0.005 ; vs . before the vacation **** p < 0.001 4. Discussion The main results of the present study were the following: 1) A short vacation of 7 days with regular exercise was well tolerated by untrained study participants, may be considered safe, and significantly improved psychological well-being. 2) Changes in HRV revealed improved parasympathetic activity. 3) Sleep quality and sleep architecture were also significantly improved. Ad H1) Vacation is referred to as a form of “macro-regeneration” and is regarded as an effective form of regeneration to reduce accumulated stress better than a single evening or a weekend off from work [ 7 ]. Our study showed that a short-term vacation with various activity programs significantly improved well-being in both activity groups. The improvement was especially pronounced in the G group, whose members felt very stressed at the start of their vacation. Our data were confirmed in a study published by Blank et al. in 2018, featuring a study design similar to the OGTS. Blank et al. described the effects of a short-term vacation on the stress level of German-speaking managers and observed positive effects of the vacation on well-being and strain reduction; the latter was more pronounced in the physically active intervention group [ 20 ]. The members of the intervention group spent four nights in a hotel and practiced various sports activities (Nordic walking or swimming and yoga or Qui-Gong) guided by a fitness instructor. The control group spent four nights at home, without working or exercising. Blank et al. noted significant reductions in strain levels by 1.3 score points in the intervention group and 0.9 in the control group. The increase in well-being and the strain reduction persisted for 45 days after the short-term vacation in both groups [ 20 ]. In our study, the stress level of the participants, evaluated with the EBF-24 questionnaire, was reduced by an average of 1.5 points in the G group and 1.3 points in the NW&EB group. We also registered positive long-term effects. The second survey of the OGTS, carried out two years later, showed that 85 percent of the study participants experienced positive long-term effects in terms of lifestyle modification, diet, physical activity, and the ability to relax. Similar positive effects of a vacation on well-being were reported in a meta-analysis published by de Bloom et al [ 7 ]. In this summary of several studies, which regrettably do not report the duration of the holiday in all cases and lack a structured activity program, the authors noted minor and rather temporary positive effects on health and well-being [ 7 ]. Regular exercise appears to be a decisive factor in improving well-being during a vacation. Our data show that regular exercise enhanced well-being to a significant extent in both study groups, regardless of the type of activity. This phenomenon was confirmed in a meta-analysis published by de Bloom et al. in 2012: the authors concluded that sporadic exercise (~ 27% of the vacation time) does not alter health parameters or well-being [ 5 ]. Our data suggest that the weekly duration of activity may exert a profound effect on the degree of improvement in well-being. The increase in well-being was more pronounced in the G group, whose mean exercise duration of 33.5 hours per week was significantly longer than the exercise duration of 14.2 hours per week in the NW&EB group. Our data appear to confirm the pre-existing notion that the regularity and duration of physical activity – and less so its intensity – improve well-being to a decisive extent [ 21 , 22 ]. Our thesis is supported by an international meta-analysis published by Schuch et al. in 2018, which examined the relationship between physical activity and the lifetime risk of developing depression. This study showed that a higher level of physical activity is liable to reduce the lifetime risk of depression [ 21 ]. Thus, hypothesis 1 , which stated that a short-term vacation with activities of moderate intensity has a positive impact on psychological well-being, was confirmed. Ad H2) Our data showed that an active short-term vacation influenced HRV positively, although the changes were significant only in the NW&EB group. SDNN in the 24-hour HRV analysis is the gold standard to stratify cardiovascular risk, and was proven to be a reliable prognostic parameter of morbidity and mortality in long-term studies. SDNN values below 50 ms are interpreted as a risk factor. Persons with values between 50 and 100 ms are considered somewhat restricted, and those with values above 100 ms are considered normal or healthy [ 15 ]. Patients with SDNN values above 100 ms had a 5.3-fold lower risk of mortality than those with an SDNN of 50 ms [ 15 ]. In our study, baseline values were reduced in both groups: 45.7 ms in the NW&EB group and 41.7 ms in the G group. After the vacation, only the NW&EB group revealed a significant increase in SDNN (+ 4 ms) ( p < 0.05 ). We assume that a longer vacation would have led to more pronounced changes in HRV in both groups. A study published by Melanson et al. in 2001, which examined the effects of a moderately to highly intensive long-term training program over 12 weeks, reported a significant increase in both, the time and the frequency domain, supports this notion [ 23 ]. In our study, the increase in HRV was more pronounced in the NW&EB group than in the G group. The NW&EB group revealed a significant increase in SDNN, while their r-MSSD and pNN50 improved to a greater extent than in the G group. Nordic walking and e-biking are traditional aerobic endurance sports. Golf involves a great deal of dynamic and isometric muscle contractions and is characterized by significantly lower exercise intensity and energy demands (3–4 METs) compared to Nordic walking or e-biking (5–6 METs) [ 4 ]. The published literature suggests that HRV is primarily influenced by aerobic exercise of a certain threshold of intensity [ 10 ]. The fact that the changes in HRV were not significant in the G group - and only the change in SDNN was significant in the NW&EB group – may be explained by the fact that the required intensity threshold or product of exercise duration and intensity was not achieved in the G group. The improvements in well-being and autonomic status observed in the present study confirm pre-existing evidence and should encourage the general population to raise their physical activity levels. The data are in line with the current 2020 ESC guidelines on sports cardiology and exercise in patients with cardiovascular disease. The guideline recommends moderately intensive aerobic exercise for at least 150 minutes per week, spread over five days, for all healthy persons. Alternatively, more intensive training units of 75 minutes, spread over three days in a week, are recommended to save time in a busy everyday life [ 24 ]. Daytime sleepiness and dejection are signs of incomplete regeneration overnight due to poor sleep quality. Altered nocturnal sympathetic activity appears to be the trigger, counteracting physiological parasympathetic predominance during sleep [ 25 ]. We derived sleep architecture from the HRV measurements. Although the changes in pNN50 and r-MSSD were not significant, there were similar improvements in both groups on the EBF-24 questionnaire; both groups experienced a reduction of tiredness and dejection. pNN50 and r-MSSD are HRV parameters to analyze autonomic functions, especially parasympathetic activity [ 25 ]. Total power and log LF/HF were also slightly increased. These data concur with previous studies, which also demonstrated the effect of exercise (strength training) on HRV in patients with stable coronary heart disease and revealed a significant increase in r-MSSD in the training group [ 26 ]. We conclude that both groups showed similar changes in HRV, although some of these did not achieve statistical significance. In combination with the significant results of the questionnaire in regard of well-being, it may be concluded that the exercise program had positive effects on ANS as well. Hypothesis , which postulated positive effects of an active vacation on HRV and ANS, was partly confirmed. Changes in HRV parameters were observed, but were not significant with the exception of SDNN in the NW&EB group. Ad H3) Sleep architecture (determined from the HRV) as well as sleep quality on the EBF-24 questionnaire were significantly improved in both groups. Sleep architecture improved from moderately to slightly disturbed sleep in the G group, and from slightly disturbed to nearly normal in the NW&EB group ( p < 0.05 ). Nocturnal PNS activity was significantly increased on the HRV spectrogram (Fig. 1 and Fig. 2 ). Our results were confirmed in a meta-analysis published by Kredlow et al. in 2016, who investigated the effects of exercise on sleep. Short-term, and especially long-term sporting activities had positive effects on various sleep characteristics, such as total sleep duration, stage 1 sleep, deep sleep, time to fall asleep, and REM sleep. These parameters were dependent on gender, age, basic activity level, duration of activity, type and time of sport exercise [ 27 ]. Hypothesis , which postulated that the combination of regular exercise and vacation has a positive effect on sleep quality and sleep architecture, was confirmed. Epidemiological studies showed that an active lifestyle protects the individual from the so-called diseasome of inactivity, and is able to increase life expectancy [ 28 , 29 ]. Remedies for physical inactivity are one of the foremost public health issues worldwide. Present and future strategies in health policy must be aimed towards increasing physical activity in leisure time and during vacations [ 30 ]. A structured training program, as provided by the study design, appears to have learning effects and has a lasting positive effect on health behavior. 85% of the study participants reported long-term changes in their activity behavior, and were more health-conscious. The second survey also revealed a notable subjective recovery effect 2–3 months after the vacation. Although the study design of the OGTS did not differentiate between “activity-related” and “holiday-related” health effects resulting from the sole absence of work stress, we suppose that vacations without an activity program achieve just minor effects on health and well-being recognizing the findings of the meta-analysis by de Bloom et al. in 2009 [ 7 ]. 5. Conclusions The present study showed that a short-term vacation of seven days with physical activities of low to moderate intensity significantly improves well-being and sleep quality in healthy persons. We also noted positive effects on the ANS and HRV. The inactive lifestyles of modern society are expected to raise the frequency of inactivity-related metabolic, cardiovascular, oncologic, psychiatric and neurodegenerative diseases. This cluster of diseases, also known as the diseasome of inactivity, can be prevented by a more physically active lifestyle in daily life. These will be the prime goal of future public health policies. Information campaigns, education programs, enlightenment and knowledge transfer by mass media will be needed to encourage greater activity in everyday life and during vacations. The East Tyrol Health Tourism Study is a preliminary step in scientific research on this health-related issue. An active short-term vacation was shown to be a safe, health-enhancing measure and served as an initial spark in achieving a more active lifestyle. Limitations Additional factors which could have affected the outcome of the study, for instance caloric intake during the holidays, the socioeconomic status or job profile of the participants were not considered. Abbreviations HRV: Heart rate variability. ECG: Electrocardiogram. OGTS: East Tyrol Health Tourism Study. NW&EB: Nordic walking and e-biking; WHO: World Health Organization. BMI: Body mass index. SDNN: standard deviation of the NN intervals in ms; pNN50: percentage of the intervals with at least 50 ms deviation from the previous interval; r-MSSD: root mean square of successive differences between normal heartbeats; log LF/HF: quotient of the logarithm of low frequency to high frequency. Spirit: standard protocol items: recommendations for interventional trials. Declarations Ethics a pproval and consent to participate : Approval was received from the ethics committee of the Leopold Franzens University of Innsbruck. Registry and the registration no. of the study/trial: (AN2013-0059 332/4.8). A written informed consent to participate was provided by all study participants prior to the study. All experiments were performed in accordance with relevant guidelines and regulations. Consent to publish: A written informed consent to publish was provided by all study participants prior to the study. Availability of data and materials: The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request. Competing interests: The authors declare that they have no competing interests. Conflicts of Interest: The authors certify that there is no conflict of interests. Funding: The project was supported by the Tyrolean Government, the East Tyrolean Tourist Board, and the participating hotels in East Tyrol (Dolomitengolf­hotel und Spa, Golfhotel Suites, Grandhotel Lienz, Hotel Moarhof, Hotel Ansitz Haidenhof, Hotel Outside, Hotel Traube, Hotel Rauter), and the Hospital of Lienz. Acknowledgement: Not applicable Author Contributions: Markus Hübner performed statistical analysis and interpretation of data, literature research, drafted and edited the paper. Peter Lechleitner was responsible for the study design, data collection, analysis and interpretation of data. Günther Neumayr was in charge of data collection, analysis, interpretation of data, drafting and revision of the paper. References United States. Dept. of Health and Human Services. Physical Activity Guidelines Advisory Committee & United States. Dept. of Health and Human Services. Physical Activity Guidelines Advisory Committee report, 2018: To the Secretary of Health and Human Services. U.S. Dept. of Health and Human Services. 2018. https://health.gov/sites/default/files/2019-09/PAG_Advisory_Committee_Report.pdf. Accessed 25 July 2022. World Health Organization. Global Strategy on Diet, Physical Activity andhealth. 2004. https://www.who.int/dietphysicalactivity/strategy/eb11344/strategy_english_web.pdf. Accessed 25 July 2022. Eller-Berndl D. Gesunder Schlaf hat eine Architektur. In: Eller-Berndl D, editor Herzratenvariabilität. 2nd ed. Vienna, Austria: Verlagshaus der Ärzte GmbH; 2015.p.83–104. Neumayr G, Lechleitner P. Effects of a one-week vacation with various activity programs on cardiovascular parameters. The Journal of sports medicine and physical fitness. 2019;59(2):335–339. https://doi.org/10.23736/S0022-4707.18.08221-X. [CrossRef] [PubMed] de Bloom J, Geurts SA, Kompier MA. Effects of short vacations, vacation activities and experiences on employee health and well-being. Stress and health : journal of the International Society for the Investigation of Stress. 2012;28(4):305–318. https://doi.org/10.1002/smi.1434. [CrossRef] [PubMed] Neumayr G, Engler C, Lunger L, et al. Effects of a One-week Vacation with Various Activity Programs on Metabolism and Adipokines. International journal of sports medicine. 2021;42(8):703–707. https://doi.org/10.1055/a-1297-4669. [CrossRef] [PubMed] de Bloom J, Kompier M, Geurts S, et al. Do we recover from vacation? Meta-analysis of vacation effects on health and well being. Journal of occupational health. 2009;51(1)13–25. https://doi.org/10.1539/joh.k8004. 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Psychotherapy and psychosomatics. 2015;84(3):167–176. https://doi.org/10.1159/000376585. [CrossRef] [PubMed] Kallus KW. Stress and Recovery: An Overview. In: Kallus KW, Kellmann M, eds. The Recovery-Stress Questionnaires: User Manual. Frankfurt am Main, Germany: Pearson Assessment & Information GmbH; 2016:27–48. Schaffran P, Kleinert J, Altfeld S, et al. Early Risk Detection of Burnout: Development of the Burnout Prevention Questionnaire for Coaches. Frontiers in psychology: 2019;10:714. https://doi.org/10.3389/fpsyg.2019.00714. [CrossRef] [PubMed] Shaffer F, Ginsberg JP. An Overview of Heart Rate Variability Metrics and Norms. Frontiers in public health. 2017;5:258. https://doi.org/10.3389/fpubh.2017.00258. [CrossRef] [PubMed] Eller-Berndl D. HRV-Analyse im Zeitbereich. In: Eller-Berndl D, editor. Herzratenvariabilität. 2nd ed. Vienna, Austria: Verlagshaus der Ärzte GmbH; 2015. p.29–31. Eller-Berndl D. Beispiele für den Tag-Nacht-Rhythmus im Spektrogramm. In: Eller-Berndl D, editor. Herzratenvariabilität. 2nd ed. Vienna, Austria: Verlagshaus der Ärzte GmbH; 2015.p.79–82. Eller-Berndl D. Schlafstörungen. In: Eller-Berndl D, editor. Herzraten-variabilität. 2nd ed. Vienna, Austria: Verlagshaus der Ärzte GmbH; 2015.p.90–105. Eller-Berndl D. Normaler Schlaf hat eine Architektur. In: Eller-Berndl D, editor. Herzratenvariabilität. 2nd ed. Vienna, Austria: Verlagshaus der Ärzte GmbH; 2015.p.83–85. Blank C, Gatterer K, Leichtfried V, et al. Short Vacation Improves Stress-Level and Well-Being in German-Speaking Middle-Managers-A Randomized Controlled Trial. International journal of environmental research and public health. 2018;15(1):130. https://doi.org/10.3390/ijerph15010130. [CrossRef] [PubMed] Schuch FB, Vancampfort D, Firth J, et al. Physical Activity and Incident Depression: A Meta-Analysis of Prospective Cohort Studies. The American journal of psychiatry. 2018;175(7):631–648. https://doi.org/10.1176/appi.ajp.2018.17111194. [CrossRef] [PubMed] Hu MX, Turner D, Generaal E, et al. Exercise interventions for the prevention of depression: a systematic review of meta-analyses. BMC public health. 2020:20(1):1255. https://doi.org/10.1186/s12889-020-09323-y. [CrossRef] [PubMed] Melanson EL, Freedson PS. The effect of endurance training on resting heart rate variability in sedentary adult males. European journal of applied physiology. 2001;85(5):442–449. https://doi.org/10.1007/s004210100479. [CrossRef] [PubMed] Pelliccia A, Sharma S, Gati S, et al. ESC Scientific Document Group. 2020 ESC Guidelines on sports cardiology and exercise in patients with cardiovascular disease. European heart journal. 2021;42(1):17–96. https://doi.org/10.1093/eurheartj/ehaa605. [CrossRef] [PubMed] Eller-Berndl D. Stress. In: Eller-Berndl D, editor. Herzratenvariabilität. 2nd ed. Vienna, Austria: Verlagshaus der Ärzte GmbH; 2015.p.219–235. Caruso FR, Arena R, Phillips SA, et al. Resistance exercise training improves heart rate variability and muscle performance: a randomized controlled trial in coronary artery disease patients. European journal of physical and rehabilitation medicine. 2015;51(3):281–289. [PubMed] Kredlow MA, Capozzoli MC, Hearon, BA, et al. The effects of physical activity on sleep: a meta-analytic review. Journal of behavioral medicine. 2015;38(3):427–449. https://doi.org/10.1007/s10865-015-9617-6. [CrossRef] [PubMed] Walsh NP, Gleeson M, Shephard RJ, et al. Position statement. Part one: Immune function and exercise. Exercise immunology review. 2011;17:6–63. PubMed Wen CP, Wai JP., Tsai MK, et al. Minimum amount of physical activity for reduced mortality and extended life expectancy: a prospective cohort study. Lancet (London, England). 2011;378(9798):1244–1253. https://doi.org/10.1016/S0140-6736(11)60749-6. [CrossRef] [PubMed] Blair S. N. Physical inactivity: the biggest public health problem of the 21st century. British journal of sports medicine. 2009;43(1):1–2. [PubMed] Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 27 Dec, 2022 Read the published version in BMC Public Health → Version 2 posted Editorial decision: Major revision 29 Sep, 2022 Reviews received at journal 26 Sep, 2022 Reviewers agreed at journal 17 Sep, 2022 Reviewers invited by journal 16 Sep, 2022 Editor assigned by journal 16 Sep, 2022 Editor invited by journal 05 Sep, 2022 Submission checks completed at journal 05 Sep, 2022 First submitted to journal 22 Aug, 2022 You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1946353","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[{"code":1,"date":"2022-08-17 14:33:40","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":139177754,"identity":"654f2c9d-6ef2-4e99-a7cb-037069c52076","order_by":0,"name":"Markus Hübner","email":"data:image/png;base64,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","orcid":"","institution":"Tirol Kliniken","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Markus","middleName":"","lastName":"Hübner","suffix":""},{"id":139177755,"identity":"05f5ee1a-c870-41e9-8ad3-99491666bcc0","order_by":1,"name":"Peter Lechleitner","email":"","orcid":"","institution":"Symbiomed Medical Center Lienz, and Department of Internal Medicine, Hospital Lienz","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Peter","middleName":"","lastName":"Lechleitner","suffix":""},{"id":139177756,"identity":"0efa039b-ae9c-4c98-beb7-b698d096a580","order_by":2,"name":"Günther Neumayr","email":"","orcid":"","institution":"Medical Office of Dr. Neumayr, Lienz","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Günther","middleName":"","lastName":"Neumayr","suffix":""}],"badges":[],"createdAt":"2022-08-09 18:14:16","currentVersionCode":2,"declarations":"","doi":"10.21203/rs.3.rs-1946353/v2","doiUrl":"https://doi.org/10.21203/rs.3.rs-1946353/v2","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s12889-022-14838-7","type":"published","date":"2022-12-27T18:07:58+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":26976692,"identity":"7d724c3d-39b7-4d77-a896-0cef9aa80ced","added_by":"auto","created_at":"2022-09-26 15:46:15","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":270235,"visible":true,"origin":"","legend":"\u003cp\u003eHRV spectrogram of a study participant one day before the vacation.\u003c/p\u003e","description":"","filename":"F1.png","url":"https://assets-eu.researchsquare.com/files/rs-1946353/v2/600ff5b0474dd1e57798997e.png"},{"id":26976693,"identity":"ceab41a0-c786-4513-af2e-b9513f13fdcd","added_by":"auto","created_at":"2022-09-26 15:46:15","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":236860,"visible":true,"origin":"","legend":"\u003cp\u003eHRV spectrogram of the same study participant after the one-week vacation.\u003c/p\u003e","description":"","filename":"F2.png","url":"https://assets-eu.researchsquare.com/files/rs-1946353/v2/85003fc77577baf691443bc5.png"},{"id":44715203,"identity":"47f5438a-f661-4950-85b9-ba7871910bfa","added_by":"auto","created_at":"2023-10-16 18:13:46","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":958018,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1946353/v2/6d1a2f96-5f12-4691-9d13-0f80659901e9.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Effects of a one-week vacation with various activity programs on well-being, heart rate variability, and sleep quality in healthy vacationers - an open comparative study","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eRegular physical training has positive effects on both physical and mental health, supports the individual to achieve an ongoing reduction of daily stress, and is recommended worldwide as a beneficial lifestyle measure by various health organization [\u003cspan additionalcitationids=\"CR2\" citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]. Vacation, defined by Lounsbury and Hoopes in 1986 as a temporary period of several days or weeks off from work, is also considered \u0026ldquo;healthy\u0026rdquo; for recovery from work stress, although scientific data in this regard are scarce. Scientific research investigating the impact of vacation and exercise therein on medical risk factors or established health parameters is virtually non-existent [\u003cspan additionalcitationids=\"CR5\" citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. In Western societies holiday behavior reveals a tendency towards short vacations. The question arises as to whether short-term vacations provide sufficient recovery from work and everyday stress [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eA chronic unstable balance between stress and recovery will cause exhaustion sooner or later; which cannot be compensated by a weekend or a few days off from work [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e]. Any chronic overload situation, regardless of whether it is a burnout syndrome at the emotional-psychological level or an overtraining syndrome at the physical level, causes an alteration of HRV parameters [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]. Physical training, defined by specific criteria such as regularity, duration, and the intensity of exercise above an individual threshold causes physiological adaptations in all organ systems, including autonomic regulation of the cardiac system [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. Therefore, HRV analysis is probably the most important non-invasive method to study changes in various autonomic functions [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eThe study design of the East Tyrol Health Tourism Study (OGTS) was such that all sports activities \u0026ndash; golf, Nordic walking and e-biking \u0026ndash; were of low to moderate exercise intensities and could be performed by nearly everyone, including untrained persons. The parameters investigated in this part of the OGTS, i.e. psychological well-being, HRV, and sleep quality are influenced negatively by any kind of stress and positively by the quantity of regeneration and physical activity. The purpose of the OGTS was to investigate the cumulative health effects of a one-week vacation and regular physical activity with regard to three hypotheses:\u003c/p\u003e \u003cp\u003e \u003cstrong\u003eHypothesis 1\u003c/strong\u003e \u003cp\u003e \u003cem\u003e(H1): A short-term vacation with moderately intensive sports activities has measurable and positive effects on psychological well-being.\u003c/em\u003e \u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eHypothesis 2\u003c/strong\u003e \u003cp\u003e \u003cem\u003e(H2): HRV is positively influenced by a week of active vacation.\u003c/em\u003e \u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eHypothesis 3\u003c/strong\u003e \u003cp\u003e \u003cem\u003e(H3): The quality of sleep is improved during a vacation.\u003c/em\u003e \u003c/p\u003e \u003c/p\u003e"},{"header":"2. Materials And Methods","content":"\u003cdiv class=\"Section2\" id=\"Sec3\"\u003e\n \u003ch2\u003e2.1. Design and procedure\u003c/h2\u003e\n \u003cp\u003eThe present study was part of the East Tyrol Health Tourism Study (OGTS) and investigated the effects of a short-term vacation with activity programs on psychological well-being, and various autonomic functions such as HRV and sleep quality in healthy volunteers. The cardiovascular and metabolic findings of the OGTS - describing the effects on performance capacity, blood pressure, heart rate and diastolic heart function as well as changes in metabolism and the adipokine profile - were published previously [\u003cspan class=\"CitationRef\"\u003e4\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e6\u003c/span\u003e].\u003c/p\u003e\n \u003cp\u003eIn an open comparative study, 52 healthy untrained vacationers spent a one-week active vacation in East Tyrol. All participants provided their written consent prior to the study [\u003cspan class=\"CitationRef\"\u003e4\u003c/span\u003e]. The study was approved by the ethics committee of the Medical University of Innsbruck. The study participants were accommodated at one of the eight hotels in East Tyrol, at an altitude of 670 to 980 meters above sea level [\u003cspan class=\"CitationRef\"\u003e4\u003c/span\u003e]. Three sports programs were available: golf, Nordic walking, and e-biking. Nordic walking and e-biking were combined in a single group because the exercise intensity and duration were similar. Group 1 played golf and group 2 performed Nordic walking or e-biking (NW\u0026amp;EB). Group assignment was based on the vacationer\u0026lsquo;s preference. The mean duration of exercise during an e-biking or walking tour was 2.4 hours in the NW\u0026amp;EB group. The mean duration of exercise was 33.5 h/week in the G group and 14.2 h/week in the NW\u0026amp;EB group. Every training unit was guided by a fitness instructor. The participants\u0026rsquo; daily caloric intake was not taken into account; a specific diet plan was not followed. Psychological well-being was registered on the WHO-5 well-being index, and the current stress and recovery profile with the EBF-24 questionnaire on stress and recovery. HRV analysis was performed on the basis of a 24-hour-ECG. Sleep quality was registered on the EBF-24 questionnaire, and sleep architecture was derived from the HRV analysis. All examinations were performed one day before and after the vacation. A second survey of the OGTS was carried out two years after the one-week vacation to examine long-term effects in terms of lifestyle modification, diet, physical activity, and the ability to relax. The protocol timeline is provided in \u003cstrong\u003e(\u003c/strong\u003eTable \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e\u003cstrong\u003e)\u003c/strong\u003e.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv class=\"Section2\" id=\"Sec4\"\u003e\n \u003ch2\u003e2.2. Measurements\u003c/h2\u003e\n \u003cdiv class=\"Section3\" id=\"Sec5\"\u003e\n \u003ch2\u003e2.2.1. Well-being\u003c/h2\u003e\n \u003cp\u003ePsychological well-being was assessed with the German version of the original WHO-5 well-being index of 1998. The WHO-5 questionnaire includes positively phrased feelings such as a good mood, feeling calm and relaxed, active and vigorous, fresh and rested, and interested in matters of everyday life [\u003cspan class=\"CitationRef\"\u003e12\u003c/span\u003e].\u003c/p\u003e\n \u003cp\u003eResults are expressed on a 6-point Likert scale for each item, ranging from 0 to 5: 0\u0026thinsp;=\u0026thinsp;at no time, 1\u0026thinsp;=\u0026thinsp;some of the time, 2\u0026thinsp;=\u0026thinsp;less than half the time, 3\u0026thinsp;=\u0026thinsp;more than half the time, 4\u0026thinsp;=\u0026thinsp;most of the time, 5\u0026thinsp;=\u0026thinsp;all of the time. This provides a prime score ranging from 0 to 25. The result is multiplied by 4 to obtain a standardized percentage of points, yielding a figure between 0% and 100% [\u003cspan class=\"CitationRef\"\u003e12\u003c/span\u003e].\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv class=\"Section3\" id=\"Sec6\"\u003e\n \u003ch2\u003e2.2.2. Stress and recovery\u003c/h2\u003e\n \u003cp\u003eThe recovery-stress questionnaire (EBF) used in the present study was the German version of the EBF-Basic-24-A3 / B3. The EBF-24 questionnaire comprises 12 topics: 7 of these address stress and 5 address recovery. Each topic consists of 2 items [\u003cspan class=\"CitationRef\"\u003e13\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e14\u003c/span\u003e]. We evaluated 4 of 7 topics addressing stress: feelings such as dejection, irritability, tiredness and subjective malaise, and 4 of 5 subjects addressing recovery: feelings such as relaxation, mood, satisfaction, and sleep quality. Each of the 12 subjects consisting of 2 items was mapped on a 7-point Likert scale ranging from 0 (never) to 6 (always). The questionnaire was modified to the current study design, and assessed the previous 7\u0026ndash;14 days and nights [\u003cspan class=\"CitationRef\"\u003e13\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e14\u003c/span\u003e].\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv class=\"Section3\" id=\"Sec7\"\u003e\n \u003ch2\u003e2.2.3. HRV\u003c/h2\u003e\n \u003cp\u003eHRV analysis was performed with a 24-hour-ECG (Medilog AR12 plus\u0026reg;, Schiller, Feldkirchen, Germany). The following parameters were measured: SDNN, r-MSSD, pNN50, total power and log LF/HF. SDNN: standard deviation of the NN intervals in ms; pNN50: percentage of the intervals with at least 50 ms deviation from the previous interval; r-MSSD: root mean square of successive differences between normal heartbeats; log LF/HF: quotient of the logarithm of low frequency to high frequency; total power: total number of all frequency ranges [\u003cspan class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e16\u003c/span\u003e].\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv class=\"Section3\" id=\"Sec8\"\u003e\n \u003ch2\u003e2.2.4. Sleep quality\u003c/h2\u003e\n \u003cp\u003eSleep quality was investigated by the EBF-24 questionnaire, the apnea index, and HRV analysis. The following parameters were used to assess sleep architecture from HRV analysis: nocturnal, tub shaped heart rate depression, respiratory sinus arrhythmia (ranging from 0.2 to 0.3 Hz), arousals, and periods of deep sleep [\u003cspan class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e17\u003c/span\u003e\u0026ndash;\u003cspan class=\"CitationRef\"\u003e19\u003c/span\u003e]. Based on these parameters and on HRV measurements, we determined a score ranging from 1 to 4 for each study participant (1\u0026thinsp;=\u0026thinsp;normal sleep architecture; 4\u0026thinsp;=\u0026thinsp;strongly disturbed sleep architecture).\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv class=\"Section2\" id=\"Sec9\"\u003e\n \u003ch2\u003e2.3. Statistical analysis\u003c/h2\u003e\n \u003cp\u003eIBM SPSS Statistics for Mactonish, Version 26.0 (IBM Corp., Armonk, N.Y., USA) was used for statistical analysis. Wilcoxon\u0026rsquo;s rank sum test was employed to determine \u003cem\u003ep-values\u003c/em\u003e for the before-and-after comparison. The interquartile range (IQR, 25th and 75th percentiles) was also calculated. The level of statistical significance was set to \u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.05.\u003c/em\u003e\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\u0026nbsp;\u003ctable border=\"1\" id=\"Tab1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eSpirit (standard protocol items: recommendations for interventional trials) - protocol timeline\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003ccolgroup cols=\"7\"\u003e\u003c/colgroup\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEnrolment\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eAllocation\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" colspan=\"4\"\u003e\n \u003cp\u003ePost allocation\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eTIMEPOINT\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e-t\u003c/span\u003e\u003csub\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e1\u003c/span\u003e\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003et\u003c/span\u003e\u003csub\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e1\u003c/span\u003e\u003c/sub\u003e\u003c/p\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e(1 day before vacation)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003et\u003c/span\u003e\u003csub\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e2\u003c/span\u003e\u003c/sub\u003e\u003c/p\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e(1-week vacation)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003et\u003c/span\u003e\u003csub\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e3\u003c/span\u003e\u003c/sub\u003e\u003c/p\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e(1 day after vacation)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003et\u003c/span\u003e\u003csub\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e3\u0026thinsp;\u0026minus;\u0026thinsp;4\u003c/span\u003e\u003c/sub\u003e\u003c/p\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e(up to 2 years after vacation)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eENROLMENT\u003c/strong\u003e:\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eEligibility screen\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eInformed consent\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eAllocation\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eINTERVENTIONS\u003c/strong\u003e:\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e[Golf]\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cimg src=\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAD4AAAAJCAIAAADl3AgGAAAAAXNSR0IArs4c6QAAAARnQU1BAACxjwv8YQUAAAAJcEhZcwAADsMAAA7DAcdvqGQAAACdSURBVDhP1ZKxDcQgDEVvGzo6KiZgATo6hmAJKiqWyBBMwAJMQEVHx/nEl3JKMoDzKvv/5kn2Z/2RUsLEkoveqX4chxAi54ydGXc9qPfelVLUSSlbazvkw6Me1L33VLwCa+12/qmXUhC/BHoeqM85jTGI2aO1HmNAnai1ohGCjrBDPjzqQZ2IMVIRQsDOjLveqU5v45zbt2DIVW+tL4WDjoBeWUqaAAAAAElFTkSuQmCC\"\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e[Nordic walking or e-biking]\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cimg src=\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAD4AAAAJCAIAAADl3AgGAAAAAXNSR0IArs4c6QAAAARnQU1BAACxjwv8YQUAAAAJcEhZcwAADsMAAA7DAcdvqGQAAACdSURBVDhP1ZKxDcQgDEVvGzo6KiZgATo6hmAJKiqWyBBMwAJMQEVHx/nEl3JKMoDzKvv/5kn2Z/2RUsLEkoveqX4chxAi54ydGXc9qPfelVLUSSlbazvkw6Me1L33VLwCa+12/qmXUhC/BHoeqM85jTGI2aO1HmNAnai1ohGCjrBDPjzqQZ2IMVIRQsDOjLveqU5v45zbt2DIVW+tL4WDjoBeWUqaAAAAAElFTkSuQmCC\"\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003eASSESSMENTS\u003c/strong\u003e:\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e[psychological well-being]\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e[HRV measurement]\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e[Sleep architecture]\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cspan class=\"BoldItalic\" name=\"Emphasis\" type=\"BoldItalic\"\u003e[second survey]\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eX\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n\u003c/div\u003e"},{"header":"3. Results","content":"\u003cp\u003eFifty-two persons participated in the study (30 men and 22 women). Their median age was 54.3 years. Baseline characteristics were similar in both groups. The sole significant difference was the mean exercise duration secondary to the study design. (Table \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e\u003cstrong\u003e)\u003c/strong\u003e summarizes the baseline characteristics of the study participants.\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\u0026nbsp;\u003ctable border=\"1\" id=\"Tab2\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eBaseline characteristics of the study participants before the one-week vacation\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003ccolgroup cols=\"3\"\u003e\u003c/colgroup\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eParameter\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eGolf group\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eNW\u0026amp;EB group\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNumber (n)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e22\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAge (years)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e54 (47\u0026ndash;63)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e54 (47\u0026ndash;60)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eGender (m, %)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e53\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e64\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBMI (kg/m\u0026sup2;)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e27 (23\u0026ndash;30)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e27 (22\u0026ndash;30)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eMean exercise duration/week (h)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e33.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14.2\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp\u003eResults shown as medians and interquartile range (IQR).\u003c/p\u003e\n\u003cdiv class=\"Section2\" id=\"Sec11\"\u003e\n \u003ch2\u003e3.1. WHO-5 well-being index\u003c/h2\u003e\n \u003cp\u003eBefore the vacation, the mean score on the WHO-5 well-being index was 15.3 in the G group and 16.45 in the NW\u0026amp;EB group. After the vacation, an increase of 5.29 points was noted in the G group and 3.14 points in the NW\u0026amp;EB group. The WHO-5 well-being index increased to 20.59 in the G group and 19.59 in the NW\u0026amp;EB group. The standardized percentage values increased from 60\u0026ndash;84% in the G group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001), and from 64\u0026ndash;80% in the NW\u0026amp;EB group (p\u0026thinsp;=\u0026thinsp;0.019). This corresponds to a relative percentage increase of 40% in the G group and 19% in the NW\u0026amp;EB group. The increase in the WHO-5 well-being index observed in both groups implies a significant improvement of well-being after one week of active vacation.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv class=\"Section2\" id=\"Sec12\"\u003e\n \u003ch2\u003e3.2. EBF-24\u003c/h2\u003e\n \u003cp\u003eThe questionnaire was divided into two parts: stress and recovery. A detailed group comparison of mean values is given in \u003cstrong\u003e(\u003c/strong\u003eTable \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e\u003cstrong\u003e)\u003c/strong\u003e.\u003c/p\u003e\n \u003cdiv class=\"Section3\" id=\"Sec13\"\u003e\n \u003ch2\u003e3.2.1. Stress\u003c/h2\u003e\n \u003cp\u003ePerceived stress was reduced significantly after one week in both groups. The NW\u0026amp;EB group showed a significant point reduction in all four subtests evaluated for stress \u0026ndash; \u003cem\u003edejection\u003c/em\u003e, \u003cem\u003eirritability\u003c/em\u003e, \u003cem\u003etiredness\u003c/em\u003e and \u003cem\u003esubjective malaise\u003c/em\u003e \u0026ndash; which corresponds to a reduction of stress. The group participants felt \u003cem\u003eless dejected\u003c/em\u003e (-50%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.044\u003c/em\u003e), \u003cem\u003eless irritable\u003c/em\u003e (-52%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.05\u003c/em\u003e), \u003cem\u003eless tired\u003c/em\u003e (-38%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.045\u003c/em\u003e) and \u003cem\u003eless uncomfortable\u003c/em\u003e (-42%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.006\u003c/em\u003e). Golfers, by comparison, achieved statistical significance in 3 of 4 subtests: \u003cem\u003edejection\u003c/em\u003e (-65%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.003\u003c/em\u003e), \u003cem\u003eirritability\u003c/em\u003e (-38%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.004\u003c/em\u003e) and \u003cem\u003etiredness\u003c/em\u003e (-52%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.08\u003c/em\u003e). No significant outcome was observed for \u003cem\u003esubjective malaise\u003c/em\u003e (-20%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.874\u003c/em\u003e). In a group comparison, the improvement in feelings such as \u003cem\u003edejection\u003c/em\u003e, \u003cem\u003eirritability\u003c/em\u003e and \u003cem\u003etiredness\u003c/em\u003e were more pronounced in the G group than in the NW\u0026amp;EB group. The cumulative stress value, which comprised mean values for \u003cem\u003edejection, irritability, tiredness\u003c/em\u003e and \u003cem\u003esubjective malaise\u003c/em\u003e, decreased by 6 points in the G group (from 13.73 to 7.73 points), corresponding to a percentage decrease of 43.7%. In the NW\u0026amp;EB group, this value decreased by 5.3 points (from 11.9 to 6.6 points) or 44.7%. This resulted in a decrease of ~\u0026thinsp;44% for stress reduction in the entire group (G\u0026thinsp;+\u0026thinsp;NW\u0026amp;EB).\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv class=\"Section3\" id=\"Sec14\"\u003e\n \u003ch2\u003e3.2.2. Recovery\u003c/h2\u003e\n \u003cp\u003eIn both groups, the recovery profile improved after one week. The increase in all four evaluated subtests was more significant in golfers. Group members felt \u003cem\u003emore satisfied\u003c/em\u003e, \u003cem\u003ehappier\u003c/em\u003e, \u003cem\u003emore relaxed\u003c/em\u003e, and \u003cem\u003emore rested\u003c/em\u003e than they did before the holiday. The score achieved by golfers on the EBF-24 questionnaire for \u003cem\u003esatisfaction\u003c/em\u003e increased from 7.53 to 8.97 points [relative percentage increase of 19% (\u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.003\u003c/em\u003e)]. The NW\u0026amp;EB group showed a measurable but non-significant increase of 11% in \u003cem\u003esatisfaction\u003c/em\u003e (\u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.063\u003c/em\u003e). In both groups, the study participants felt \u003cem\u003emore relaxed\u003c/em\u003e after their vacation. The average increase in \u003cem\u003erelaxation\u003c/em\u003e was 32% in the G group and 35% in the NW\u0026amp;EB group (\u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.005\u003c/em\u003e). Improvements in \u003cem\u003ehappiness\u003c/em\u003e (+\u0026thinsp;25%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.007\u003c/em\u003e), \u003cem\u003erelaxation\u003c/em\u003e (+\u0026thinsp;35%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.001\u003c/em\u003e) and \u003cem\u003esleep quality\u003c/em\u003e (+\u0026thinsp;19%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.007\u003c/em\u003e) were more pronounced in the NW\u0026amp;EB group than in the G group. The latter achieved significant increases in their scores for \u003cem\u003ehappiness\u003c/em\u003e (+\u0026thinsp;24%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.021\u003c/em\u003e), \u003cem\u003erelaxation\u003c/em\u003e (+\u0026thinsp;32%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.002\u003c/em\u003e) and \u003cem\u003esleep quality\u003c/em\u003e (+\u0026thinsp;21%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.029\u003c/em\u003e). The cumulative recovery value, which comprised the mean values for \u003cem\u003esatisfaction\u003c/em\u003e, \u003cem\u003ehappiness\u003c/em\u003e, \u003cem\u003erelaxation\u003c/em\u003e and \u003cem\u003esleep quality\u003c/em\u003e, resulted in a point increase of 6.7 (from 28.4 to 35.1 points) in the G group, which corresponds to a percentage increase of 23.6%. This value increased by 5.8 points (from 27 to 32.8 points) or 21.5% in the NW\u0026amp;EB group. The entire group (G\u0026thinsp;+\u0026thinsp;NW\u0026amp;EB) experienced an improvement of ~\u0026thinsp;23% in regeneration and recovery.\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv class=\"Section2\" id=\"Sec15\"\u003e\n \u003ch2\u003e3.3. HRV\u003c/h2\u003e\n \u003cp\u003eThere was a small but non-significant change in all measured HRV parameters, except for a significant improvement of SDNN in the NW\u0026amp;EB group. The changes were similar in both groups. r-MSSD, pNN50, log LF/HF and total power improved slightly but non-significantly in terms of increased parasympathetic activity in both groups. The mean SDNN was high at the start of the vacation in the NW\u0026amp;EB group, and increased significantly from 45.67 ms to 49.67 ms, corresponding to a relative percentage increase of 9% (\u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.05\u003c/em\u003e). Details of the HRV parameters are shown in \u003cstrong\u003e(\u003c/strong\u003eTable \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e\u003cstrong\u003e)\u003c/strong\u003e.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv class=\"Section2\" id=\"Sec16\"\u003e\n \u003ch2\u003e3.4. Sleep quality based on the EBF-24 questionnaire, HRV analysis, and apnea index\u003c/h2\u003e\n \u003cdiv class=\"Section3\" id=\"Sec17\"\u003e\n \u003ch2\u003e3.4.1. Sleep architecture (HRV)\u003c/h2\u003e\n \u003cp\u003eThe score of the G group for sleep architecture was 2.44 (on a scale from 1 to 4) before the vacation, and decreased to 2.2 (-10%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.034\u003c/em\u003e) after the vacation. This result signifies an improvement from a \u003cem\u003emoderately\u003c/em\u003e to a \u003cem\u003eslightly disturbed\u003c/em\u003e sleep architecture. The decrease was even more pronounced in the NW\u0026amp;EB group. The mean value in this group decreased from 2.35 to 1.8 (-23%, \u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.012\u003c/em\u003e). Sleep architecture changed significantly; it improved from a \u003cem\u003emoderately\u003c/em\u003e to a \u003cem\u003eslightly disturbed\u003c/em\u003e sleep architecture in the G group, and from a \u003cem\u003eslightly disturbed\u003c/em\u003e to a \u003cem\u003enearly normal\u003c/em\u003e sleep architecture in the NW\u0026amp;EB group. A clear increase in nocturnal parasympathetic activity was noted on the HRV spectrogram. \u003cstrong\u003e(Fig.\u0026nbsp;1)\u003c/strong\u003e and \u003cstrong\u003e(Fig.\u0026nbsp;2)\u003c/strong\u003e show changes in sleep architecture throughout the vacation in one study participant.\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv class=\"Section3\" id=\"Sec18\"\u003e\n \u003ch2\u003e3.4.2. Sleep quality EBF-24\u003c/h2\u003e\n \u003cp\u003eAll study participants felt \u003cem\u003emore rested\u003c/em\u003e in the morning and \u003cem\u003eless tired\u003c/em\u003e after one week of active vacation compared to the beginning. The number of points on the EBF-24 questionnaire increased from 7.58 to 9.13 after the vacation in the G group (\u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.029\u003c/em\u003e), and from 7.55 to 8.95 in the NW\u0026amp;EB (\u003cem\u003ep\u0026thinsp;=\u0026thinsp;0.007\u003c/em\u003e). The improvement of sleep quality was significant in both groups, but more pronounced in the NW\u0026amp;EB group.\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv class=\"Section3\" id=\"Sec19\"\u003e\n \u003ch2\u003e3.4.3. Apnea index\u003c/h2\u003e\n \u003cp\u003eThe apnea index was calculated on the basis of the HRV analysis, and revealed no significant change in either group. The number of nocturnal apneas was only reduced among golfers (from\u003c/p\u003e\n \u003cp\u003e6.66 to 5.32). However, the apnea index increased from 7.64 to 7.84 in the NW\u0026amp;EB group. Specific data on sleep quality are summarized in \u003cstrong\u003e(\u003c/strong\u003eTable \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e\u003cstrong\u003e)\u003c/strong\u003e.\u003c/p\u003e\n \u003cp\u003eHRV spectrogram of a study participant one day before the vacation. The red ellipsoidal marking indicates a low frequency density in the HF spectrum (0.15-0.4 Hz), whereas the weak yellow coloring indicates reduced nocturnal PNS activity. X-axis: time of the HRV analysis; y-axis: frequency spectrum in Hz; Hz: Hertz; HF: high frequency. This spectrogram shows poor sleep quality and diminished nocturnal parasympathetic activity.\u003c/p\u003e\n \u003cp\u003eThe red ellipsoidal marking highlights the increased frequency density in the HF spectrum (0.15-0.4 Hz). Increasing yellow and light red coloring express good nocturnal parasympathetic activity. X-axis: time of the HRV analysis; y-axis: frequency spectrum in Hz; Hz: Hertz; HF: high frequency. This spectrogram shows improved sleep quality with demonstrable nocturnal parasympathetic activity.\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\n \u003cdiv align=\"left\" class=\"colspec\"\u003e\u003cbr\u003e\u003c/div\u003e\u0026nbsp;\u003ctable border=\"1\" id=\"Tab3\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eHeart rate variability, well-being and sleep quality in the golf and Nordic walking / e-biking groups before and after the vacation.\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003ccolgroup cols=\"6\"\u003e\u003c/colgroup\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" rowspan=\"2\"\u003e\n \u003cp\u003eParameter\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" colspan=\"3\"\u003e\n \u003cp\u003eGolf group (n\u0026thinsp;=\u0026thinsp;30)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003eNW\u0026amp;EB group (n\u0026thinsp;=\u0026thinsp;22)\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eBefore the vacation\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eAfter the vacation\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003eBefore the vacation\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eAfter the vacation\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEBF-24 stress\u003c/p\u003e\n \u003cp\u003eDejection\u003c/p\u003e\n \u003cp\u003eIrritability\u003c/p\u003e\n \u003cp\u003eTiredness\u003c/p\u003e\n \u003cp\u003eSubjective malaise\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.89 (1\u0026ndash;4)\u003c/p\u003e\n \u003cp\u003e3.21 (2\u0026ndash;4)\u003c/p\u003e\n \u003cp\u003e4.32 (2.5\u0026ndash;5)\u003c/p\u003e\n \u003cp\u003e3.32 (1.5\u0026ndash;4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1 (0\u0026ndash;1.75) ***\u003c/p\u003e\n \u003cp\u003e2 (1\u0026ndash;2) ***\u003c/p\u003e\n \u003cp\u003e2.07 (0.25\u0026ndash;3) **\u003c/p\u003e\n \u003cp\u003e2.67 (1.25\u0026ndash;4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e1.91 (0\u0026ndash;3)\u003c/p\u003e\n \u003cp\u003e3.23 (2\u0026ndash;4.75)\u003c/p\u003e\n \u003cp\u003e3.27 (2\u0026ndash;4.75)\u003c/p\u003e\n \u003cp\u003e3.50 (2\u0026ndash;5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.95 (0\u0026ndash;1) *\u003c/p\u003e\n \u003cp\u003e1.55 (0\u0026ndash;2) **\u003c/p\u003e\n \u003cp\u003e2.05 (1\u0026ndash;3) *\u003c/p\u003e\n \u003cp\u003e2.05 (1.25\u0026ndash;2) **\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEBF-24 recovery\u003c/p\u003e\n \u003cp\u003eHappiness\u003c/p\u003e\n \u003cp\u003eRelaxation\u003c/p\u003e\n \u003cp\u003eSatisfaction\u003c/p\u003e\n \u003cp\u003eSleep quality\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6.37 (5.5\u0026ndash;8)\u003c/p\u003e\n \u003cp\u003e6.89 (5.5\u0026ndash;8)\u003c/p\u003e\n \u003cp\u003e7.53 (6\u0026ndash;9.5)\u003c/p\u003e\n \u003cp\u003e7.58 (7\u0026ndash;9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7.87 (6.25\u0026ndash;9) *\u003c/p\u003e\n \u003cp\u003e9.10 (8\u0026ndash;10) ***\u003c/p\u003e\n \u003cp\u003e8.97 (8\u0026ndash;10) ***\u003c/p\u003e\n \u003cp\u003e9.13 (8.25\u0026ndash;11) *\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e5.32 (3\u0026ndash;8)\u003c/p\u003e\n \u003cp\u003e6.41 (4\u0026ndash;8)\u003c/p\u003e\n \u003cp\u003e7.77 (6.25\u0026ndash;9.75)\u003c/p\u003e\n \u003cp\u003e7.55 (6\u0026ndash;10)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6.64 (5\u0026ndash;8.75) **\u003c/p\u003e\n \u003cp\u003e8.68 (8\u0026ndash;10) ***\u003c/p\u003e\n \u003cp\u003e8.59 (8\u0026ndash;10)\u003c/p\u003e\n \u003cp\u003e8.95 (8\u0026ndash;11) **\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eWHO-5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e15.3 (12\u0026ndash;18)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e20.59 (18.25\u0026ndash;22) ****\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e16.45 (13.5\u0026ndash;20)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e19.59 (17.25\u0026ndash;21.75) *\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSDNN (ms)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e41.74 (32.05\u0026ndash;48.85)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e40.88 (34.7\u0026ndash;46.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e45.67 (36\u0026ndash;53)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e49.67 (40\u0026ndash;56) *\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003epNN50 (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.87 (0.5\u0026ndash;2.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3.23 (0.57\u0026ndash;3.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e2.74 (0.77\u0026ndash;2.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3.67 (0.71\u0026ndash;5.54)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003er-MSSD (ms)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e20.09 (12.6\u0026ndash;19.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e20.91 (12.8\u0026ndash;24.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e19.51 (16\u0026ndash;21.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e21.84 (17.7\u0026ndash;26.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003elog LF/HF (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.68 (0.59\u0026ndash;0.82)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.67 (0.54\u0026ndash;0.79)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e0.61 (0.5\u0026ndash;0.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.60 (0.44\u0026ndash;0.73)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTotal power (ms2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2021 (1224\u0026ndash;2619)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2375 (1341\u0026ndash;2979)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e3212 (2415\u0026ndash;4111)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3555 (2468\u0026ndash;4714)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSleep architecture\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.44 (2\u0026ndash;3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.20 (2\u0026ndash;3) *\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e2.35 (2\u0026ndash;3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.80 (1\u0026ndash;2) *\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSleep quality (EBF-24)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7.58 (7\u0026ndash;9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e8.68 (7\u0026ndash;9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e7.55 (6\u0026ndash;10)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e8.95 (8\u0026ndash;11)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eApnea index\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7.04 (0.71\u0026ndash;10.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e5.32 (0.5\u0026ndash;9.12)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003e7.64 (3\u0026ndash;8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7.84 (4.6\u0026ndash;10.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n \u003cp\u003e\u003c/p\u003e\n \u003cp\u003eResults shown as mean values rounded to two decimal places and interquartile range (IQR) in the golf vs. NW\u0026amp;EB groups. NW\u0026amp;EB: Nordic walking and e-biking; WHO-5: World Health Organization-5 index of well-being; SDNN: standard deviation of the NN intervals in ms; pNN50: percentage of the intervals with at least 50 ms deviation from the previous interval; r-MSSD: root mean square of successive differences between normal heartbeats; log LF/HF: quotient of the logarithm of low frequency to high frequency; total power: total number of all frequency ranges; Sleep architecture (HRV): evaluation of sleep quality based on heart rate variability; Sleep quality (EBF-24): evaluation of sleep quality based on the stress-recovery questionnaire; Apnea index: number of cessations of breathing; ms: milliseconds; ms\u0026sup2;: milliseconds squared. \u003cem\u003ep-value\u003c/em\u003e within the group: \u003cem\u003evs\u003c/em\u003e. before the vacation * \u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.05\u003c/em\u003e; \u003cem\u003evs\u003c/em\u003e. before the vacation ** \u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.01\u003c/em\u003e; \u003cem\u003evs\u003c/em\u003e. before the vacation *** \u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.005\u003c/em\u003e; \u003cem\u003evs\u003c/em\u003e. before the vacation **** \u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.001\u003c/em\u003e\u003c/p\u003e\n \u003cp\u003e\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e"},{"header":"4. Discussion","content":"\u003cp\u003eThe main results of the present study were the following:\u003c/p\u003e \u003cp\u003e1) A short vacation of 7 days with regular exercise was well tolerated by untrained study participants, may be considered safe, and significantly improved psychological well-being.\u003c/p\u003e \u003cp\u003e2) Changes in HRV revealed improved parasympathetic activity.\u003c/p\u003e \u003cp\u003e3) Sleep quality and sleep architecture were also significantly improved.\u003c/p\u003e \u003cp\u003e \u003cem\u003eAd H1)\u003c/em\u003e Vacation is referred to as a form of \u0026ldquo;macro-regeneration\u0026rdquo; and is regarded as an effective form of regeneration to reduce accumulated stress better than a single evening or a weekend off from work [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Our study showed that a short-term vacation with various activity programs significantly improved well-being in both activity groups. The improvement was especially pronounced in the G group, whose members felt very stressed at the start of their vacation.\u003c/p\u003e \u003cp\u003eOur data were confirmed in a study published by Blank et al. in 2018, featuring a study design similar to the OGTS. Blank et al. described the effects of a short-term vacation on the stress level of German-speaking managers and observed positive effects of the vacation on well-being and strain reduction; the latter was more pronounced in the physically active intervention group [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]. The members of the intervention group spent four nights in a hotel and practiced various sports activities (Nordic walking or swimming and yoga or Qui-Gong) guided by a fitness instructor. The control group spent four nights at home, without working or exercising. Blank et al. noted significant reductions in strain levels by 1.3 score points in the intervention group and 0.9 in the control group. The increase in well-being and the strain reduction persisted for 45 days after the short-term vacation in both groups [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eIn our study, the stress level of the participants, evaluated with the EBF-24 questionnaire, was reduced by an average of 1.5 points in the G group and 1.3 points in the NW\u0026amp;EB group. We also registered positive long-term effects. The second survey of the OGTS, carried out two years later, showed that 85 percent of the study participants experienced positive long-term effects in terms of lifestyle modification, diet, physical activity, and the ability to relax.\u003c/p\u003e \u003cp\u003eSimilar positive effects of a vacation on well-being were reported in a meta-analysis published by de Bloom et al [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. In this summary of several studies, which regrettably do not report the duration of the holiday in all cases and lack a structured activity program, the authors noted minor and rather temporary positive effects on health and well-being [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eRegular exercise appears to be a decisive factor in improving well-being during a vacation. Our data show that regular exercise enhanced well-being to a significant extent in both study groups, regardless of the type of activity. This phenomenon was confirmed in a meta-analysis published by de Bloom et al. in 2012: the authors concluded that sporadic exercise (~\u0026thinsp;27% of the vacation time) does not alter health parameters or well-being [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Our data suggest that the weekly duration of activity may exert a profound effect on the degree of improvement in well-being. The increase in well-being was more pronounced in the G group, whose mean exercise duration of 33.5 hours per week was significantly longer than the exercise duration of 14.2 hours per week in the NW\u0026amp;EB group. Our data appear to confirm the pre-existing notion that the regularity and duration of physical activity \u0026ndash; and less so its intensity \u0026ndash; improve well-being to a decisive extent [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e, \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e]. Our thesis is supported by an international meta-analysis published by Schuch et al. in 2018, which examined the relationship between physical activity and the lifetime risk of developing depression. This study showed that a higher level of physical activity is liable to reduce the lifetime risk of depression [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e].\u003c/p\u003e \u003cp\u003e \u003cem\u003eThus, hypothesis\u003c/em\u003e \u003cspan refid=\"FPar1\" class=\"InternalRef\"\u003e\u003cem\u003e1\u003c/em\u003e\u003c/span\u003e, \u003cem\u003ewhich stated that a short-term vacation with activities of moderate intensity has a positive impact on psychological well-being, was confirmed.\u003c/em\u003e\u003c/p\u003e \u003cp\u003e \u003cem\u003eAd H2)\u003c/em\u003e Our data showed that an active short-term vacation influenced HRV positively, although the changes were significant only in the NW\u0026amp;EB group. SDNN in the 24-hour HRV analysis is the gold standard to stratify cardiovascular risk, and was proven to be a reliable prognostic parameter of morbidity and mortality in long-term studies. SDNN values below 50 ms are interpreted as a risk factor. Persons with values between 50 and 100 ms are considered somewhat restricted, and those with values above 100 ms are considered normal or healthy [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. Patients with SDNN values above 100 ms had a 5.3-fold lower risk of mortality than those with an SDNN of 50 ms [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. In our study, baseline values were reduced in both groups: 45.7 ms in the NW\u0026amp;EB group and 41.7 ms in the G group. After the vacation, only the NW\u0026amp;EB group revealed a significant increase in SDNN (+\u0026thinsp;4 ms) (\u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.05\u003c/em\u003e). We assume that a longer vacation would have led to more pronounced changes in HRV in both groups. A study published by Melanson et al. in 2001, which examined the effects of a moderately to highly intensive long-term training program over 12 weeks, reported a significant increase in both, the time and the frequency domain, supports this notion [\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eIn our study, the increase in HRV was more pronounced in the NW\u0026amp;EB group than in the G group. The NW\u0026amp;EB group revealed a significant increase in SDNN, while their r-MSSD and pNN50 improved to a greater extent than in the G group. Nordic walking and e-biking are traditional aerobic endurance sports. Golf involves a great deal of dynamic and isometric muscle contractions and is characterized by significantly lower exercise intensity and energy demands (3\u0026ndash;4 METs) compared to Nordic walking or e-biking (5\u0026ndash;6 METs) [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]. The published literature suggests that HRV is primarily influenced by aerobic exercise of a certain threshold of intensity [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. The fact that the changes in HRV were not significant in the G group - and only the change in SDNN was significant in the NW\u0026amp;EB group \u0026ndash; may be explained by the fact that the required intensity threshold or product of exercise duration and intensity was not achieved in the G group.\u003c/p\u003e \u003cp\u003eThe improvements in well-being and autonomic status observed in the present study confirm pre-existing evidence and should encourage the general population to raise their physical activity levels. The data are in line with the current 2020 ESC guidelines on sports cardiology and exercise in patients with cardiovascular disease. The guideline recommends moderately intensive aerobic exercise for at least 150 minutes per week, spread over five days, for all healthy persons. Alternatively, more intensive training units of 75 minutes, spread over three days in a week, are recommended to save time in a busy everyday life [\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eDaytime sleepiness and dejection are signs of incomplete regeneration overnight due to poor sleep quality. Altered nocturnal sympathetic activity appears to be the trigger, counteracting physiological parasympathetic predominance during sleep [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e]. We derived sleep architecture from the HRV measurements. Although the changes in pNN50 and r-MSSD were not significant, there were similar improvements in both groups on the EBF-24 questionnaire; both groups experienced a reduction of tiredness and dejection. pNN50 and r-MSSD are HRV parameters to analyze autonomic functions, especially parasympathetic activity [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e]. Total power and log LF/HF were also slightly increased. These data concur with previous studies, which also demonstrated the effect of exercise (strength training) on HRV in patients with stable coronary heart disease and revealed a significant increase in r-MSSD in the training group [\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eWe conclude that both groups showed similar changes in HRV, although some of these did not achieve statistical significance. In combination with the significant results of the questionnaire in regard of well-being, it may be concluded that the exercise program had positive effects on ANS as well.\u003c/p\u003e \u003cp\u003e \u003cstrong\u003eHypothesis\u003c/strong\u003e \u003cp\u003e, \u003cem\u003ewhich postulated positive effects of an active vacation on HRV and ANS, was partly confirmed. Changes in HRV parameters were observed, but were not significant with the exception of SDNN in the NW\u0026amp;EB group.\u003c/em\u003e\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cem\u003eAd H3)\u003c/em\u003e Sleep architecture (determined from the HRV) as well as sleep quality on the EBF-24 questionnaire were significantly improved in both groups. Sleep architecture improved from moderately to slightly disturbed sleep in the G group, and from slightly disturbed to nearly normal in the NW\u0026amp;EB group (\u003cem\u003ep\u0026thinsp;\u0026lt;\u0026thinsp;0.05\u003c/em\u003e). Nocturnal PNS activity was significantly increased on the HRV spectrogram (Fig.\u0026nbsp;1 and \u003cb\u003eFig.\u0026nbsp;2\u003c/b\u003e).\u003c/p\u003e \u003cp\u003eOur results were confirmed in a meta-analysis published by Kredlow et al. in 2016, who investigated the effects of exercise on sleep. Short-term, and especially long-term sporting activities had positive effects on various sleep characteristics, such as total sleep duration, stage 1 sleep, deep sleep, time to fall asleep, and REM sleep. These parameters were dependent on gender, age, basic activity level, duration of activity, type and time of sport exercise [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e].\u003c/p\u003e \u003cp\u003e \u003cstrong\u003eHypothesis\u003c/strong\u003e \u003cp\u003e, \u003cem\u003ewhich postulated that the combination of regular exercise and vacation has a positive effect on sleep quality and sleep architecture, was confirmed.\u003c/em\u003e\u003c/p\u003e \u003c/p\u003e \u003cp\u003eEpidemiological studies showed that an active lifestyle protects the individual from the so-called diseasome of inactivity, and is able to increase life expectancy [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e, \u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e]. Remedies for physical inactivity are one of the foremost public health issues worldwide. Present and future strategies in health policy must be aimed towards increasing physical activity in leisure time and during vacations [\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eA structured training program, as provided by the study design, appears to have learning effects and has a lasting positive effect on health behavior. 85% of the study participants reported long-term changes in their activity behavior, and were more health-conscious. The second survey also revealed a notable subjective recovery effect 2\u0026ndash;3 months after the vacation.\u003c/p\u003e \u003cp\u003eAlthough the study design of the OGTS did not differentiate between \u0026ldquo;activity-related\u0026rdquo; and \u0026ldquo;holiday-related\u0026rdquo; health effects resulting from the sole absence of work stress, we suppose that vacations without an activity program achieve just minor effects on health and well-being recognizing the findings of the meta-analysis by de Bloom et al. in 2009 [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e].\u003c/p\u003e"},{"header":"5. Conclusions","content":"\u003cp\u003eThe present study showed that a short-term vacation of seven days with physical activities of low to moderate intensity significantly improves well-being and sleep quality in healthy persons. We also noted positive effects on the ANS and HRV. The inactive lifestyles of modern society are expected to raise the frequency of inactivity-related metabolic, cardiovascular, oncologic, psychiatric and neurodegenerative diseases. This cluster of diseases, also known as the diseasome of inactivity, can be prevented by a more physically active lifestyle in daily life. These will be the prime goal of future public health policies. Information campaigns, education programs, enlightenment and knowledge transfer by mass media will be needed to encourage greater activity in everyday life and during vacations.\u003c/p\u003e \u003cp\u003eThe East Tyrol Health Tourism Study is a preliminary step in scientific research on this health-related issue. An active short-term vacation was shown to be a safe, health-enhancing measure and served as an initial spark in achieving a more active lifestyle.\u003c/p\u003e \u003cp\u003e \u003cstrong\u003eLimitations\u003c/strong\u003e \u003cp\u003eAdditional factors which could have affected the outcome of the study, for instance caloric intake during the holidays, the socioeconomic status or job profile of the participants were not considered.\u003c/p\u003e \u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eHRV: Heart rate variability. ECG: Electrocardiogram. OGTS: East Tyrol Health Tourism Study.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003eNW\u0026amp;EB: Nordic walking and e-biking; WHO: World Health Organization. BMI: Body mass index. SDNN: standard deviation of the NN intervals in ms; pNN50: percentage of the intervals with at least 50 ms deviation from the previous interval; r-MSSD: root mean square of successive differences between normal heartbeats; log LF/HF: quotient of the logarithm of low frequency to high frequency. Spirit: standard protocol items: recommendations for interventional trials.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics a\u003c/strong\u003e\u003cstrong\u003epproval\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003eand consent to participate\u003c/strong\u003e\u003cstrong\u003e:\u003c/strong\u003e Approval was received from the ethics committee of the Leopold Franzens University of Innsbruck.\u0026nbsp;Registry and the registration no. of the study/trial:\u0026nbsp;(AN2013-0059 332/4.8). A written informed consent\u0026nbsp;to participate\u0026nbsp;was\u0026nbsp;provided by all study participants prior to the study. All experiments were performed in accordance with relevant guidelines and regulations.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to publish:\u003c/strong\u003e A written informed consent\u0026nbsp;to publish\u0026nbsp;was\u0026nbsp;provided by all study participants prior to the study.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials:\u003c/strong\u003e The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests:\u003c/strong\u003e The authors declare that they have no competing interests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflicts of Interest:\u003c/strong\u003e The authors certify that there is no conflict of interests.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding:\u003c/strong\u003e The project was supported by the Tyrolean Government, the East Tyrolean Tourist Board, and the participating hotels in East Tyrol (Dolomitengolf\u0026shy;hotel und Spa, Golfhotel Suites, Grandhotel Lienz, Hotel Moarhof, Hotel Ansitz Haidenhof, Hotel Outside, Hotel Traube, Hotel Rauter), and the Hospital of Lienz.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgement:\u0026nbsp;\u003c/strong\u003eNot applicable\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor Contributions:\u003c/strong\u003e Markus H\u0026uuml;bner performed statistical analysis and interpretation of data, literature research, drafted and edited the paper. Peter Lechleitner was responsible for the study design, data collection, analysis and interpretation of data. G\u0026uuml;nther Neumayr was in charge of data collection, analysis, interpretation of data, drafting and revision of the paper.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eUnited States. Dept. of Health and Human Services. Physical Activity Guidelines Advisory Committee \u0026amp; United States. Dept. of Health and Human Services. Physical Activity Guidelines Advisory Committee report, 2018: To the Secretary of Health and Human Services. U.S. Dept. of Health and Human Services. 2018. https://health.gov/sites/default/files/2019-09/PAG_Advisory_Committee_Report.pdf. Accessed 25 July 2022.\u003c/li\u003e\n\u003cli\u003eWorld Health Organization. Global Strategy on Diet, Physical Activity andhealth. 2004. https://www.who.int/dietphysicalactivity/strategy/eb11344/strategy_english_web.pdf. Accessed 25 July 2022.\u003c/li\u003e\n\u003cli\u003eEller-Berndl D. Gesunder Schlaf hat eine Architektur. In: Eller-Berndl D, editor Herzratenvariabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p.83\u0026ndash;104. \u003c/li\u003e\n\u003cli\u003eNeumayr G, Lechleitner P. Effects of a one-week vacation with various activity programs on cardiovascular parameters. The Journal of sports medicine and physical fitness. 2019;59(2):335\u0026ndash;339. https://doi.org/10.23736/S0022-4707.18.08221-X. [CrossRef] [PubMed]\u003c/li\u003e\n\u003cli\u003ede Bloom J, Geurts SA, Kompier MA. Effects of short vacations, vacation activities and experiences on employee health and well-being. Stress and health : journal of the International Society for the Investigation of Stress. 2012;28(4):305\u0026ndash;318. https://doi.org/10.1002/smi.1434. [CrossRef] [PubMed]\u003c/li\u003e\n\u003cli\u003eNeumayr G, Engler C, Lunger L, et al. 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Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p. 211\u0026ndash;218.\u003c/li\u003e\n\u003cli\u003eEller-Berndl D. Epigenetik- Interface zwischen Genen und Umwelt. In: Eller-Berndl D, editor. Herzraten-variabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p.107\u0026ndash;121. \u003c/li\u003e\n\u003cli\u003eAubert AE, Seps B, Beckers F. Heart rate variability in athletes. Sports medicine (Auckland, N.Z.). 2003;33(12):889\u0026ndash;919. https://doi.org/10.2165/00007256-200333120-00003. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eTopp CW, \u0026Oslash;stergaard SD, S\u0026oslash;ndergaard S, et al. The WHO-5 Well-Being Index: a systematic review of the literature. Psychotherapy and psychosomatics. 2015;84(3):167\u0026ndash;176. https://doi.org/10.1159/000376585. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eKallus KW. Stress and Recovery: An Overview. In: Kallus KW, Kellmann M, eds. The Recovery-Stress Questionnaires: User Manual. Frankfurt am Main, Germany: Pearson Assessment \u0026amp; Information GmbH; 2016:27\u0026ndash;48. \u003c/li\u003e\n\u003cli\u003eSchaffran P, Kleinert J, Altfeld S, et al. Early Risk Detection of Burnout: Development of the Burnout Prevention Questionnaire for Coaches. Frontiers in psychology: 2019;10:714. https://doi.org/10.3389/fpsyg.2019.00714. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eShaffer F, Ginsberg JP. An Overview of Heart Rate Variability Metrics and Norms. Frontiers in public health. 2017;5:258. https://doi.org/10.3389/fpubh.2017.00258. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eEller-Berndl D. HRV-Analyse im Zeitbereich. In: Eller-Berndl D, editor. Herzratenvariabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015. p.29\u0026ndash;31.\u003c/li\u003e\n\u003cli\u003eEller-Berndl D. Beispiele f\u0026uuml;r den Tag-Nacht-Rhythmus im Spektrogramm. In: Eller-Berndl D, editor. Herzratenvariabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p.79\u0026ndash;82.\u003c/li\u003e\n\u003cli\u003e Eller-Berndl D. Schlafst\u0026ouml;rungen. In: Eller-Berndl D, editor. Herzraten-variabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p.90\u0026ndash;105.\u003c/li\u003e\n\u003cli\u003eEller-Berndl D. Normaler Schlaf hat eine Architektur. In: Eller-Berndl D, editor. Herzratenvariabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p.83\u0026ndash;85. \u003c/li\u003e\n\u003cli\u003eBlank C, Gatterer K, Leichtfried V, et al. Short Vacation Improves Stress-Level and Well-Being in German-Speaking Middle-Managers-A Randomized Controlled Trial. International journal of environmental research and public health. 2018;15(1):130. https://doi.org/10.3390/ijerph15010130. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eSchuch FB, Vancampfort D, Firth J, et al. Physical Activity and Incident Depression: A Meta-Analysis of Prospective Cohort Studies. The American journal of psychiatry. 2018;175(7):631\u0026ndash;648. https://doi.org/10.1176/appi.ajp.2018.17111194. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eHu MX, Turner D, Generaal E, et al. Exercise interventions for the prevention of depression: a systematic review of meta-analyses. BMC public health. 2020:20(1):1255. https://doi.org/10.1186/s12889-020-09323-y. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eMelanson EL, Freedson PS. The effect of endurance training on resting heart rate variability in sedentary adult males. European journal of applied physiology. 2001;85(5):442\u0026ndash;449. https://doi.org/10.1007/s004210100479. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003ePelliccia A, Sharma S, Gati S, et al. ESC Scientific Document Group. 2020 ESC Guidelines on sports cardiology and exercise in patients with cardiovascular disease. European heart journal. 2021;42(1):17\u0026ndash;96. https://doi.org/10.1093/eurheartj/ehaa605. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eEller-Berndl D. Stress. In: Eller-Berndl D, editor. Herzratenvariabilit\u0026auml;t. 2nd ed. Vienna, Austria: Verlagshaus der \u0026Auml;rzte GmbH; 2015.p.219\u0026ndash;235.\u003c/li\u003e\n\u003cli\u003eCaruso FR, Arena R, Phillips SA, et al. Resistance exercise training improves heart rate variability and muscle performance: a randomized controlled trial in coronary artery disease patients. European journal of physical and rehabilitation medicine. 2015;51(3):281\u0026ndash;289. [PubMed] \u003c/li\u003e\n\u003cli\u003eKredlow MA, Capozzoli MC, Hearon, BA, et al. The effects of physical activity on sleep: a meta-analytic review. Journal of behavioral medicine. 2015;38(3):427\u0026ndash;449. https://doi.org/10.1007/s10865-015-9617-6. [CrossRef] [PubMed]\u003c/li\u003e\n\u003cli\u003eWalsh NP, Gleeson M, Shephard RJ, et al. Position statement. Part one: Immune function and exercise. Exercise immunology review. 2011;17:6\u0026ndash;63. PubMed \u003c/li\u003e\n\u003cli\u003eWen CP, Wai JP., Tsai MK, et al. Minimum amount of physical activity for reduced mortality and extended life expectancy: a prospective cohort study. Lancet (London, England). 2011;378(9798):1244\u0026ndash;1253. https://doi.org/10.1016/S0140-6736(11)60749-6. [CrossRef] [PubMed] \u003c/li\u003e\n\u003cli\u003eBlair S. N. Physical inactivity: the biggest public health problem of the 21st century. British journal of sports medicine. 2009;43(1):1\u0026ndash;2. [PubMed]\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-public-health","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pubh","sideBox":"Learn more about [BMC Public Health](http://bmcpublichealth.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/pubh/default.aspx","title":"BMC Public Health","twitterHandle":"@BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"physical activity, vacation, psychological well-being, stress, heart rate variability, sleep quality","lastPublishedDoi":"10.21203/rs.3.rs-1946353/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1946353/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjectives: \u003c/strong\u003eThis open comparative study aimed to analyze the effects of a one-week vacation with various activity programs on well-being, heart rate variability (HRV) and sleep quality in healthy vacationers.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods:\u003c/strong\u003e 52 healthy untrained vacationers spent a one-week vacation with regular exercise in East Tyrol. Exercise was performed on six of seven days. The study participants were divided into a) Group 1, playing golf (G), and b) Group 2 performing Nordic walking or e-biking (NW\u0026amp;EB). Well-being was measured with the WHO-5 well-being-index; stress and recovery status was obtained with the EBF-24-questionnaire (recovery-stress questionnaire). HRV-parameters were measured with a 24-hour-ECG (electrocardiogram). Sleep quality was derived from the EBF-24 questionnaire and sleep architecture from HRV-analysis. Examinations were performed one day before and after the vacation.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003eWell-being significantly improved in the G group (+40%, p \u0026lt; 0.001) and NW\u0026amp;EB group (+19%, p = 0.019). The stress and recovery profile also improved significantly in both groups (stress-decrease: -43.7% G group; -44.7% NW\u0026amp;EB group; recovery-increase: +23.6% G group; +21.5% NW\u0026amp;EB group). Except for the SDNN (standard deviation of the NN interval), no significant change was noted in HRV-parameters. SDNN improved significantly only in the NW\u0026amp;EB group (+9%, p \u0026lt; 0.05). Sleep quality (+21% G group, p = 0.029; +19% NW\u0026amp;EB group, p = 0.007) and architecture (-10% G group, p = 0.034; -23% NW\u0026amp;EB group, p = 0.012) significantly improved in both groups.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003e A short-term vacation with regular exercise was well tolerated by the study participants and improved well-being, sleep quality, HRV and autonomic regulation.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTrial registration: \u003c/strong\u003eRegistry and the registration no. of the study/trial: Approval was received from the ethics committee of the Leopold Franzens University of Innsbruck (AN2013-0059 332/4.8)\u003c/p\u003e","manuscriptTitle":"Effects of a one-week vacation with various activity programs on well-being, heart rate variability, and sleep quality in healthy vacationers - an open comparative study","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2022-09-26 15:46:13","doi":"10.21203/rs.3.rs-1946353/v2","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revision","date":"2022-09-29T12:13:56+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2022-09-26T21:04:44+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"794d6570-9faa-4c7c-9da3-7fe773e79bf6","date":"2022-09-17T13:31:24+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2022-09-16T13:12:57+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2022-09-16T12:23:07+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2022-09-05T18:25:19+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2022-09-05T18:22:54+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Public Health","date":"2022-08-22T14:41:42+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-public-health","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pubh","sideBox":"Learn more about [BMC Public Health](http://bmcpublichealth.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/pubh/default.aspx","title":"BMC Public Health","twitterHandle":"@BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"81cc290e-0717-4a8a-b87b-1e684c8af122","owner":[],"postedDate":"September 26th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2023-10-16T18:10:58+00:00","versionOfRecord":{"articleIdentity":"rs-1946353","link":"https://doi.org/10.1186/s12889-022-14838-7","journal":{"identity":"bmc-public-health","isVorOnly":false,"title":"BMC Public Health"},"publishedOn":"2022-12-27 18:07:58","publishedOnDateReadable":"December 27th, 2022"},"versionCreatedAt":"2022-09-26 15:46:13","video":"","vorDoi":"10.1186/s12889-022-14838-7","vorDoiUrl":"https://doi.org/10.1186/s12889-022-14838-7","workflowStages":[]},"version":"v2","identity":"rs-1946353","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1946353","identity":"rs-1946353","version":["v2"]},"buildId":"WrCJVZZCHTDjtuVLN7oU0","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}