Preparation of protein imprinted gelatin/N-Vinylformamide hydrogel membrane protein grafted on polyurethane

Preparation of protein imprinted gelatin/N-Vinylformamide hydrogel membrane protein grafted on polyurethane | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Preparation of protein imprinted gelatin/N-Vinylformamide hydrogel membrane protein grafted on polyurethane Otar Mikautadze, Dali Chkheidze, Temur Kotishadze, Mikheil Surguladze This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4010064/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Using polyurethane (PU) as the carrier, membrane protein as the template, N-Vinylformamide (NVF) as the monomer, and 1,4-Butanediol diglycidyl ether (BDDE) as the crosslinker, in the presence of gelatin, and initiated by ultraviolet radiation, the hydrogel PU-NVF-Gelatin was prepared. The effects of monomer concentration, crosslinker concentration, and gelatin concentration on the adsorption capacity for the membrane protein and the imprinting efficiency were studied. The results showed that the maximum adsorption capacity for the membrane protein on the PU-grafted imprinted hydrogel was achieved when the monomer mass fraction was 5%, the crosslinker mass fraction was 3%, and the gelatin mass fraction was 0.6%. Materials Chemistry PU NVF Gelatin hydrogel membrane protein Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4010064","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":276111778,"identity":"e5393d79-e42f-445d-9c6f-16635d8fbb3e","order_by":0,"name":"Otar Mikautadze","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAxUlEQVRIiWNgGAWjYNACgwQGfgYGNiJVs0G1SDaQpoUhgcHgALFa+OWbn326UZAmb3wj+dmDDxUM8vxiB/BrkWxjM56dY5BjuO1GmrnhjDMMhjNnJ+DXYnCMwZg5x6CCcduNBDNp3jaGBIPbBLWwfwZpsd88I/0bsVp4QLbkJG6QyCHSFsm2nGKglrTkGWfelEnOOCNB2C/8zMc3M+f8Sbbtb0/fJvGhwkaeX5qAFgQQAKuUIFY52L4DpKgeBaNgFIyCkQQA2Rs9s6NIqqUAAAAASUVORK5CYII=","orcid":"","institution":"","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Otar","middleName":"","lastName":"Mikautadze","suffix":""},{"id":276111779,"identity":"8ac417da-5cd2-4c88-a3f3-0da3ef263962","order_by":1,"name":"Dali Chkheidze","email":"","orcid":"","institution":"","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Dali","middleName":"","lastName":"Chkheidze","suffix":""},{"id":276111780,"identity":"4e671e08-10e5-4a10-810a-1806faf2dbb0","order_by":2,"name":"Temur Kotishadze","email":"","orcid":"","institution":"","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Temur","middleName":"","lastName":"Kotishadze","suffix":""},{"id":276111781,"identity":"b91bd80f-0614-405a-9b59-5f0a61e8538e","order_by":3,"name":"Mikheil Surguladze","email":"","orcid":"","institution":"","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mikheil","middleName":"","lastName":"Surguladze","suffix":""}],"badges":[],"createdAt":"2024-03-04 01:34:15","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false,"coiExplicitlySet":false},"doi":"10.21203/rs.3.rs-4010064/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4010064/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":52025509,"identity":"9841a024-7386-429b-80fa-0b7ced94439e","added_by":"auto","created_at":"2024-03-05 15:48:08","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":265081,"visible":true,"origin":"","legend":"","description":"","filename":"NewMicrosoftWordDocument2.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4010064/v1_covered_623b9420-2955-4304-93cc-7188fac02c80.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003ePreparation of protein imprinted gelatin/N-Vinylformamide hydrogel membrane protein grafted on polyurethane\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Kutaisi International University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"PU, NVF, Gelatin, hydrogel, membrane protein","lastPublishedDoi":"10.21203/rs.3.rs-4010064/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4010064/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eUsing polyurethane (PU) as the carrier, membrane protein as the template, N-Vinylformamide (NVF) as the monomer, and 1,4-Butanediol diglycidyl ether (BDDE) as the crosslinker, in the presence of gelatin, and initiated by ultraviolet radiation, the hydrogel PU-NVF-Gelatin was prepared. 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