Cell-based assays for potential prognostic biomarkers in PDAC

preprint OA: closed
Full text JSON View at publisher
AI-generated summary by claude@2026-07, 2026-07-16

This protocol details methods for cell-based assays using PDAC cell cultures and viral transduction to identify potential prognostic biomarkers for patient stratification.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

AI-generated deep summary by claude@2026-07, 2026-07-16 · read from full text

This protocols.io paper describes cell-based assays intended to evaluate potential prognostic biomarkers in pancreatic ductal adenocarcinoma (PDAC), focusing on assay implementation rather than reporting clinical outcomes. The high-level approach involves performing cell-based experimental measurements designed to support biomarker assessment, but the provided text does not include specific study participants, sample sources, experimental conditions, or results. A major limitation stated by the excerpt is that it mainly contains webpage/protocol metadata and code rather than substantive experimental or analytic details. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Abstract The associated publication reports proteogenomic analysis of human pancreatic ductal adenocarcinoma (PDAC), where we provided significantly mutated genes (SMGs)/biomarkers, cellular pathways, and cell types as potential therapeutic targets to improve stratification of patients with PDAC. This protocol describes the detailed methods for cell-based assays for potential prognostic biomarkers in PDAC, including cell culture, viral transduction, and cell-based assays.
Full text 5,647 characters · extracted from preprint-html · click to expand
:root { --font-inter: 'Inter', 'Roboto', sans-serif, Symbol; --font-monospace: monospace; --font-roboto: 'Roboto', sans-serif, Symbol; --font-sans-serif: sans-serif; } html, body, div, span, h1, h2, h3, h4, h5, h6, p, ol, ul, li, table, caption, tbody, tfoot, thead, tr, th, td {margin: 0;padding: 0;border: 0;font-size: 100%;font: inherit;vertical-align: baseline;} html {font-size: 14px;line-height: 1.5;} body {font-size: 14px;line-height: 1.5;font-family: var(--font-Roboto);} ol, ul {list-style: none;} blockquote, q {quotes: none;} blockquote:before, blockquote:after, q:before, q:after {content: '';content: none;} img {max-width: 100%;} table {border-collapse: collapse;border-spacing: 0;} b {font-weight: bold;} a {color: #2c71d6;text-decoration: none;} p {margin: 10px 0 10px 0;} .no-select {-o-user-select: none;-moz-user-select: none;-khtml-user-select: none;-webkit-user-select: none;user-select: none;} * {box-sizing: border-box;} @keyframes blink {0% {opacity: .1;}20% {opacity: 1;}100% {opacity: .1;}} .main-preloader{position:fixed;display: flex;top:0;bottom:0;left:0;right:0;align-items: center;justify-content: center;z-index:200000000;text-align:center;background:#F2F3F6;} .main-preloader.dark{background:#363636;} .main-preloader.hidden {display: none;} .main-preloader .mp-logo{height:100px;width:100px;} Cell-based assays for potential prognostic biomarkers in PDAC V.1 {"@context":"https://schema.org","@id":"https://www.protocols.io/#org","@type":"Organization","logo":{"@type":"ImageObject","height":"1550","url":"https://www.protocols.io/img/protocols-og.jpg","width":"1920"},"name":"protocols.io","url":"https://www.protocols.io/"} {"@context":"https://schema.org","@id":"https://www.protocols.io/#website","@type":"WebSite","name":"protocols.io","publisher":{"@id":"https://www.protocols.io/#org"},"url":"https://www.protocols.io/"} {"@context":"https://schema.org","@id":"https://www.protocols.io/view/strong-cell-based-assays-for-potential-prognostic-4r3l29wq3v1y/v1#webpage","@type":"WebPage","about":{"@id":"https://www.protocols.io/#org"},"description":"Cell-based assays for potential prognostic biomarkers in PDAC\n. The associated publication reports pro. Read full protocol, steps, and materials on protocols.io","isPartOf":{"@id":"https://www.protocols.io/#website"},"name":"Cell-based assays for potential prognostic biomarkers in PDAC\n V.1","url":"https://www.protocols.io/view/strong-cell-based-assays-for-potential-prognostic-4r3l29wq3v1y/v1"} {"@context":"https://schema.org","@id":"https://www.protocols.io/view/strong-cell-based-assays-for-potential-prognostic-d38t8rwn#article","@type":"Article","author":{"@type":"Person","affiliation":"Dev","name":"Protocols Importer"},"dateModified":"2023-01-19T11:52:46Z","datePublished":"2023-01-19T11:52:46Z","description":"Cell-based assays for potential prognostic biomarkers in PDAC\n. The associated publication reports pro. Read full protocol, steps, and materials on protocols.io","headline":"Cell-based assays for potential prognostic biomarkers in PDAC\n V.1","image":{"@type":"ImageObject","url":"https://files.protocols.io/webapp/images/q2ckeg/meta/protocolsio.png"},"mainEntityOfPage":{"@id":"https://www.protocols.io/view/strong-cell-based-assays-for-potential-prognostic-d38t8rwn#webpage"},"publisher":{"@id":"https://www.protocols.io/#org"}} var WebappBundlePath = "https:\/\/files.protocols.io\/webapp\/bundles\/production-eb29340\/"; var AMPLITUDE_API_KEY="c250ed1d862cb9c01587e1f779a32c9";var FFMPEG_HOST="https://ffmpeg.protocols.io";var FILES_CDN_URL="https://files.protocols.io";var FILES_S3_BUCKET="protocols-io-files";var FILES_S3_PREFIX="external";var GA_MEASUREMENT_ID="G-33ZGV5CT0B";var GOOGLE_CLIENT_ID="182746271041-rdtjk0b1h9706r0fgpq1o2h55lgbo8un.apps.googleusercontent.com";var HAS_SSR= false ;var HOST_ROLE= 1 ;var HOST_ROLES={"HOST_DEV":2,"HOST_LOCAL":3,"HOST_PROD":1};var IMAGE_BASE_PATH="https://files.protocols.io/webapp/images/q2ckeg/";var NATS_SERVER="wss://ws.protocols.io";var ORCID_CLIENT_ID="APP-7405TP4958GFSZXV";var ORCID_URL="https://orcid.org/oauth/authorize";var RECAPTCHA_SITE_ID="6LeMF6YUAAAAAOYzOqfc9Ml3PdTgIzO1oDdIwCYc";var SITE_URL="https://www.protocols.io";var SPRINGERNATURE_CLIENT_ID="protocolsio";var SPRINGERNATURE_REDIRECT="https://www.protocols.io/api/v1/auth/springernature/authcallback";var SPRINGERNATURE_URL="https://idp.springernature.com";var STRIPE_PUB_KEY="pk_live_51PfMxdRshFFmaQRVGeDsSX7NFFLMkMuqr17SxP6JcJuieglmQYHPgM7cz0XdO91IEVP9k4E4MC7PZB2ATyuuaOy800ivYpRURi";var UIPE="ccW9Kn73Dw3C4V1lovN9dnriPRBEq/u77eY3wEAkK0M=";var VPC_ALLOW_REGENTS_LINKING= false ;var VPC_DISABLED_INVITE_COLLEAGUES= false ;var VPC_DISABLED_PUBLISHING= false ;var VPC_DISABLED_SIGN_UP= false ;var VPC_DISABLED_WORKSPACE_CREATION= false ;var VPC_EXTERNAL_STORAGES= null ;var VPC_FORWARDED_VIEW_URL_PREFIX="";var VPC_MAIN_CLOUD_URL="https://www.protocols.io";var VPC_REQUIRE_SIGN_IN= false ;var VPC_USE_MAIN_GLOBAL_SEARCH= false ;var WEBAPP_VERSION="production-eb29340";var YJS_WEBSOCKET_URL="wss://www.protocols.io";var __stripeGlobal= null ;var cookie_session_guid="F50C126E80B911F188280A58A9FEAC02";var gosessid="F50C116E80B911F188280A58A9FEAC02";var is_premium= false ;var loadedAmplitude= false ;var loadedGA= false ;var logged_in= false ;var session_guid="";var show_loader= true ;var user_theme="default"; var is_grecaptcha_loaded = false; if (!Element.prototype.matches) Element.prototype.matches = Element.prototype.msMatchesSelector; if (!Element.prototype.closest) Element.prototype.closest = function (selector) {var el = this; while (el) {if (el.matches(selector)) return el; el = el.parentElement;}};

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

Ask this paper AI returns verbatim quotes from the full text · source: preprint-html

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.

Source provenance

europepmc
last seen: 2026-05-19T01:45:01.086888+00:00