A Multipurpose Next-Generation BPA Analysis and Tracking System with Spatial Data Integration

A Multipurpose Next-Generation BPA Analysis and Tracking System with Spatial Data Integration | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article A Multipurpose Next-Generation BPA Analysis and Tracking System with Spatial Data Integration Yaman Yazici This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7653736/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Bisphenol A (BPA) is a widely used industrial chemical and an endocrine-disrupting contaminant of increasing environmental and public health concern (1)(2). Traditional laboratory methods for BPA detection (e.g. HPLC or LC–MS) are highly sensitive but require expensive equipment, extensive sample preparation, and expert personnel. Rapid point-of-care tests exist but are typically qualitative with high detection limits, leaving a critical gap for quick, on-site, sensitive measurements. Here we present a spatially informed, multi-purpose BPA analysis and monitoring system that integrates a novel fluorogenic lateral-flow immunoassay (LFIA) with a handheld spectrofluorometric reader and a GIS-enabled data platform. The portable system can detect BPA in water and biological fluids at low part-per- billion levels (limit of detection ~ 1 ng/mL) without the need for laboratory infrastructure. We describe the design and fabrication of a fluorescent immunosensor strip and its reader device, optimization of assay conditions, and validation against benchmark methods. Case studies in environmental water monitoring, food packaging safety, and human exposure assessment demonstrate the system’s versatility. The real-time geospatial data integration allows mapping of BPA pollution hotspots, supporting rapid regulatory response and public health interventions. Our results show that the system yields semi-quantitative fluorescence readings correlating with BPA concentration, with high specificity and reproducibility. This next-generation BPA monitoring approach offers a cost-effective, field-deployable tool to strengthen environmental surveillance and reduce human exposure, and it can be readily adapted to detect other harmful pollutants in the future. Clinical Trial Number Clinical trial number not applicable. Physical sciences/Chemistry Earth and environmental sciences/Environmental sciences Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7653736","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":534275128,"identity":"bdcbe0e5-1b41-4e48-b734-4d0cc9b61973","order_by":0,"name":"Yaman 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Traditional laboratory methods for BPA detection (e.g. HPLC or LC–MS) are highly sensitive but require expensive equipment, extensive sample preparation, and expert personnel. Rapid point-of-care tests exist but are typically qualitative with high detection limits, leaving a critical gap for quick, on-site, sensitive measurements. Here we present a spatially informed, multi-purpose BPA analysis and monitoring system that integrates a novel fluorogenic lateral-flow immunoassay (LFIA) with a handheld spectrofluorometric reader and a GIS-enabled data platform. The portable system can detect BPA in water and biological fluids at low part-per- billion levels (limit of detection ~ 1 ng/mL) without the need for laboratory infrastructure. We describe the design and fabrication of a fluorescent immunosensor strip and its reader device, optimization of assay conditions, and validation against benchmark methods. 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