EFFECT OF NEIYI RECIPE ON CYTOKINES AND VEGF mRNA EXPRESSION LEVELS IN ENDOMETRIOTIC RATS

Objective:To investigate the regulating effect of Neiyi Recipe (NYF) on cytokines and vascular endothelial growth factor (VEGF) mRNA expression levels in endometriotic rats. Method: According to Cummings AM method, animal models with endometriosis was established. IL-6 ,IL-8 and IL-10 levels were determined by using double antibody sandwich enzyme-linked immunosorbent assay. The VEGF mRNA expression levels were examined by dot blot. Result: The serum IL-6 levels, peritoneal fluid IL-8 levels and peritoneal macrophage amounts were remarkably higher in model group than those in the control group, and the production of IL-6 by lipopolysaccharide(LPS)-activated peritoneal macrophages was significantly higher. The weights of endometriotic tissue and peritoneal macrophage amounts were remarkably lower in NYR treated group compared with that of untreated group, and the levels of IL-6 by LPS-activated peritoneal macrophages were significantly reduced. The LPS-stimulated IL-10 secretion in peritoneal macrophages from model group was significantly lower compared with that of control group and NYR treated group. The VEGF mRNA expression levels of endometriotic tissue were significantly higher in model group compared with those of NYR treated group. Conclusion: The abnormal of IL-6, IL-8 and IL-10 levels in endometriosis may be related to the pathogenesis of the disease. VEGF mRNA excessive expression had relation with blood vessel production of endometriotic tissue. NYR significantly restraines the growth of endometriotic tissue and VEGF mRNA excessive expression of endometriotic tissue, reduces blood vessel production of endometriotic tissue, and has remarkably regulating role in cytokines in endometriosis.