WAC-A New GBM Tumour Suppressor- Induces GBM Cell Apoptosis and Promotes Autophagy

Abstract WAC is closely related to the occurrence and development of tumors. However, its role in human glioblastoma (GBM) and its potential regulatory mechanisms have not been investigated. This study demonstrated that WAC is downregulated in GBM, and its low expression predicts a poor prognosis. We investigated the effect of WAC on the proliferation of glioma cells through the CCK-8 assay, EdU incorporation and cell formation. The effects of WAC on apoptosis and autophagy in glioma were demonstrated by flow cytometry, TUNEL detection, immunofluorescence, q-PCR, WB and scanning electron microscopy. We found that overexpression of WAC inhibited proliferation of glioma cells, promoted apoptosis and induced autophagy. Therefore, WAC is likely to play a role as a new regulatory molecule in glioma.