Eleutheroside-b and e in Acanthopanax Senticosus has Anti-PRV Activity via an Enhanced Body Immune Response in Vivo

Background: Acanthopanax senticosus extract (Eleutheroside-b and e, Acanthopanax senticosus extract, AS) is a multi-functional medicine involving in antiviral response in rodents and humans. Pseudorabies virus (PRV) is essential pathogens especially in swine farms. However, little is known about the biological functions of AS in vivo during PRV infection. MethodsIn order to confirm function of AS during PRV infection in vivo. We investigated that AS treatment (2mg/g, 4mg/g and 6mg/g) mice for 5 days affecting the mouse survival time during PRV infection. Furthermore, we investigated that AS treatment (4mg/g) mice for 1 day, 3 days and 5 days, respectively, affected the mouse survival time during PRV infection. We then treated mice with AS (4 mg/g) for 5 days and then challenged with PRV. Three days after PRV infection, we harvested mouse-samples to analyze PRV copies, performed pathological and immunohistochemical assays and the expression of immune-genes.ResultsWe found that AS treatment decreased the PRV copies and degree of pathological damage in mice brains and lungs. The AS treatment also stimulated a high level of IFN-γ, IL-2, and CCL-5 in the rodent serum, but suppressed IL-10 expression, suggesting that AS enhances the immunity response. Conclusionswe showed that AS promoted the anti-PRV ability in vivo by activating the expression of immunity genes. In summary, these findings offer a novel understanding of the immunity response during PRV infection.