1 TESLA MRI AND MIP ANALYSIS FOR CUPRIZONE-INDUCED DEMYELINATION ASSESEMENT IN C57BL/6 MICE

1 TESLA MRI AND MIP ANALYSIS FOR CUPRIZONE-INDUCED DEMYELINATION ASSESEMENT IN C57BL/6 MICE | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article 1 TESLA MRI AND MIP ANALYSIS FOR CUPRIZONE-INDUCED DEMYELINATION ASSESEMENT IN C57BL/6 MICE Veronika Volodina, Petr Zaitsev, Stanislav Antonov, Lyudmila Borets, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7078127/v2 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 2 posted 5 You are reading this latest preprint version Show more versions Abstract Purpose This study aimed to develop and validate an imaging-based approach using 1Tesla MRI to assess brain lesion area in a cuprizone-induced demyelination model of multiple sclerosis in mice. Methods Demyelination was induced in C57Bl/6 mice through a cuprizone-supplemented diet. Magnetic resonance imaging was performed regularly using a 1 Tesla permanent magnet system to monitor disease progression. Following the cuprizone feeding, mice received intraperitoneal injections of vindeburnol for 60 days, with continued MRI monitoring throughout the treatment period. Brain lesion areas were quantified using maximum intensity projection (MIP) analysis of axial and sagittal images, with contrast enhancement achieved through administration of gadopentetate dimeglumine (Magnevsit ®) Results After 90 days on the cuprizone diet, demyelination was confirmed by an increase in contrast- enhanced lesion areas on 1Tesla MRI, elevated clinical scores and histology. A subsequent 60-day course of vindeburnol treatment led to a 1.2-fold improvement in recovery compared to the spontaneous recovery group. Statistically significant reductions in both lesion area and signal intensity were observed in the vindeburnol-treated group compared to the untreated control (P < 0.001) and the spontaneous recovery group (P < 0.05). Conclusion Contrast-enhanced 1Tesla MRI combined with MIP analysis proved to be a reliable and effective method for quantifying brain lesions and evaluating therapeutic response in the cuprizone- induced demyelination model. cuprizone-induced toxic model multiple sclerosis 1Tesla MRI maximal intensity projections demyelination vindeburnol Full Text Supplementary Files SFig1.png SFig2.png FigS3.png ElectronicSupplementaryMaterial.docx Cite Share Download PDF Status: Under Revision Version 2 posted Reviewers agreed at journal 31 Oct, 2025 Reviewers invited by journal 31 Oct, 2025 Editor assigned by journal 26 Oct, 2025 First submitted to journal 26 Oct, 2025 Editorial decision: Major revisions 26 Aug, 2025 You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7078127","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":538109786,"identity":"cbf263f8-001f-4278-829f-e56b56779b73","order_by":0,"name":"Veronika Volodina","email":"","orcid":"","institution":"A N Bach Institute of Biochemistry RAS: Institut biohimii imeni A N Baha Rossijskoj akademii nauk","correspondingAuthor":false,"prefix":"","firstName":"Veronika","middleName":"","lastName":"Volodina","suffix":""},{"id":538109787,"identity":"51e8ee2e-e9ff-4ad2-98e3-d839c45768e4","order_by":1,"name":"Petr 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Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"cuprizone-induced toxic model, multiple sclerosis, 1Tesla MRI, maximal intensity projections, demyelination, vindeburnol","lastPublishedDoi":"10.21203/rs.3.rs-7078127/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7078127/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003ePurpose\u003c/h2\u003e\u003cp\u003eThis study aimed to develop and validate an imaging-based approach using 1Tesla MRI to assess brain lesion area in a cuprizone-induced demyelination model of multiple sclerosis in mice.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e\u003cp\u003eDemyelination was induced in C57Bl/6 mice through a cuprizone-supplemented diet. Magnetic resonance imaging was performed regularly using a 1 Tesla permanent magnet system to monitor disease progression. Following the cuprizone feeding, mice received intraperitoneal injections of vindeburnol for 60 days, with continued MRI monitoring throughout the treatment period. Brain lesion areas were quantified using maximum intensity projection (MIP) analysis of axial and sagittal images, with contrast enhancement achieved through administration of gadopentetate dimeglumine (Magnevsit \u0026reg;)\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e\u003cp\u003eAfter 90 days on the cuprizone diet, demyelination was confirmed by an increase in contrast- enhanced lesion areas on 1Tesla MRI, elevated clinical scores and histology. A subsequent 60-day course of vindeburnol treatment led to a 1.2-fold improvement in recovery compared to the spontaneous recovery group. Statistically significant reductions in both lesion area and signal intensity were observed in the vindeburnol-treated group compared to the untreated control (P\u0026thinsp;\u0026lt;\u0026thinsp;0.001) and the spontaneous recovery group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05).\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e\u003cp\u003eContrast-enhanced 1Tesla MRI combined with MIP analysis proved to be a reliable and effective method for quantifying brain lesions and evaluating therapeutic response in the cuprizone- induced demyelination model.\u003c/p\u003e","manuscriptTitle":"1 TESLA MRI AND MIP ANALYSIS FOR CUPRIZONE-INDUCED DEMYELINATION ASSESEMENT IN C57BL/6 MICE","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2025-11-04 01:57:27","doi":"10.21203/rs.3.rs-7078127/v2","editorialEvents":[{"type":"communityComments","content":0},{"type":"reviewerAgreed","content":"","date":"2025-10-31T14:57:19+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-10-31T14:07:02+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-10-27T03:14:11+00:00","index":"","fulltext":""},{"type":"submitted","content":"Molecular Imaging and Biology","date":"2025-10-26T14:56:26+00:00","index":"","fulltext":""},{"type":"decision","content":"Major revisions","date":"2025-08-26T09:58:13+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"molecular-imaging-and-biology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"mibi","sideBox":"Learn more about [Molecular Imaging and Biology](http://link.springer.com/journal/11307)","snPcode":"11307","submissionUrl":"https://www.editorialmanager.com/mibi/default2.aspx","title":"Molecular Imaging and Biology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}},{"code":"","date":"2025-08-26 13:57:04","doi":"","editorialEvents":[{"type":"editorAssigned","content":"","date":"2025-08-26T13:57:04+00:00","index":"","fulltext":""},{"type":"decision","content":"Major revisions","date":"2025-08-26T09:58:13+00:00","index":"","fulltext":""},{"type":"submitted","content":"Molecular Imaging and Biology","date":"2025-07-30T14:45:27+00:00","index":"","fulltext":""}],"status":"private","journal":{"display":true,"email":"[email protected]","identity":"molecular-imaging-and-biology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"mibi","sideBox":"Learn more about [Molecular Imaging and Biology](http://link.springer.com/journal/11307)","snPcode":"11307","submissionUrl":"https://www.editorialmanager.com/mibi/default2.aspx","title":"Molecular Imaging and Biology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}},{"code":1,"date":"2025-08-12 01:23:30","doi":"10.21203/rs.3.rs-7078127/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"reviewerAgreed","content":"","date":"2025-08-06T14:58:31+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-08-06T13:37:05+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-07-30T23:01:28+00:00","index":"","fulltext":""},{"type":"submitted","content":"Molecular Imaging and Biology","date":"2025-07-30T14:45:27+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"molecular-imaging-and-biology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"mibi","sideBox":"Learn more about [Molecular Imaging and Biology](http://link.springer.com/journal/11307)","snPcode":"11307","submissionUrl":"https://www.editorialmanager.com/mibi/default2.aspx","title":"Molecular Imaging and Biology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"4119b614-89a1-4b5f-9884-cc74e5adf5a5","owner":[],"postedDate":"November 4th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"in-revision","subjectAreas":[],"tags":[],"updatedAt":"2025-12-01T18:45:23+00:00","versionOfRecord":[],"versionCreatedAt":"2025-11-04 01:57:27","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-7078127","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7078127","identity":"rs-7078127","version":["v2"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}