A Parameter Study of Ultrasound Assisted Enzymatic Esterification

A Parameter Study of Ultrasound Assisted Enzymatic Esterification | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article A Parameter Study of Ultrasound Assisted Enzymatic Esterification Anamaria Vartolomei, Ioan Calinescu, Mircea Vinatoru, Adina Ionuta Gavrila This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-955645/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 26 Jan, 2022 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract This work is focused on the study of esterification parameters for the ultrasound assisted synthesis of isoamyl acetate catalyzed by lipase Lipozyme 435 in a continuous loop reactor. Investigating the influence of different parameters shows that a higher concentration of ester (462 mg/gmixture) can be obtained at temperature 50 °C, flow rate 0.16 mL/min. The best ultrasonication conditions are: continuously applied sonication for a short time (20 minutes), ultrasound power 32 mW, and amplitude 20 %. The enzyme can be successfully reused tree times without loss of enzyme activity. Reaction kinetics for isoamyl acetate ultrasound assisted production showed that satisfactory reaction concentration (close to the equilibrium concentrations) could be reached in short reaction times (2 h). Ultrasound assisted enzymatic esterification is consequently a cleaner and a faster process. Applied Biochemistry Polymer Science Parameter Ultrasound Assisted Enzymatic Esterification synthesis influence equilibrium concentrations Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 26 Jan, 2022 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Major revision 03 Dec, 2021 Reviews received at journal 30 Nov, 2021 Reviewers agreed at journal 25 Nov, 2021 Reviews received at journal 24 Nov, 2021 Reviewers agreed at journal 27 Oct, 2021 Reviewers invited by journal 13 Oct, 2021 Editor assigned by journal 13 Oct, 2021 Editor invited by journal 08 Oct, 2021 Submission checks completed at journal 08 Oct, 2021 First submitted to journal 04 Oct, 2021 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-955645","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":55997043,"identity":"bd56f352-74cb-4c55-a22a-3d732603c949","order_by":0,"name":"Anamaria Vartolomei","email":"","orcid":"","institution":"University Politehnica of Bucharest","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Anamaria","middleName":"","lastName":"Vartolomei","suffix":""},{"id":55997044,"identity":"fcdc5a76-3fa4-40f5-acba-6e2848e20656","order_by":1,"name":"Ioan Calinescu","email":"","orcid":"","institution":"University 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