Fatty Acids Production under Stress Condition by Halotolerant Bacillus: Extraction and Application

Microbial lipids or single cell oils are produced by some microorganisms such as microalgae, Bacillus bacteria, fungi and yeasts. These compounds can be straight or branched, monounsaturated, or polyunsaturated and in addition to essential unsaturated fatty acids that are used in the pharmaceutical, medical, cosmetic, and food industries, other fatty acids such as biodiesel and their derivatives also have different applications. In this experiment, fatty acids production was studied by Bacillus halotolerant in different media and the type of them were investigated by Fourier Transform Infrared and Gas Chromatography/Mass Spectrometry analysis. Different kinds of fatty acids with different applications were detected like omega-3 (α-Linolenic acid and Eicosapentaenoic acid), omega-6 (γ-Linolenic acid and Linoleic acid), myristoleic acid, palmitic acid, stearic acid, cis-hexadecenoic, cis-octadecenoic acid, and so on. The effect of metals and nanoparticles for promotion of fatty acid were also investigated in this work. the data showed that the maximum variety of fatty acids and production were related to lead-containing medium.