The effect of probiotics oral administration during gestation on infant gut microbiota in early life

The effect of probiotics oral administration during gestation on infant gut microbiota in early life | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The effect of probiotics oral administration during gestation on infant gut microbiota in early life Yongchuang Lin, Yang Chai, Lulu Wu, Yimi Li, Qilian Long, Xuan Gong, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3989775/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Probiotics have been widely used by pregnant women, but the effect of probiotics on the infant gut microbiota has not been clearly confirmed.This study aimed at investigating the effects of probiotics oral administration during the third trimester on infants' gut microbiota within the first 6 months following vaginal delivery. Methods Healthy Chinese pregnant women were recruited and randomly divided into the probiotic group and the control group. The first-pass meconium samples and the fecal samples of infants were collected by trained personnel on 3 days, 14 days, 6 months following vaginal birth. The 16SrRNA gene sequencing technology was applied to analyze the infants' gut microbiota. Results 1. Firmicutes and Proteobacteria were the most dominant phyla in both control and probiotic groups within the first 6 months of life. 2. The diversity of the infants gut microbiota had no difference between the two groups at any time points during the first 6 months of life. 3. There was a lower relative abundance of Bacteroidetes of the probiotic group infants compared with that of the control group infants on 0 day, 14 days as well as 6 months. 4. The probiotic group infants displayed a higher Firmicutes to Bacteroidetes ratio(F/B ratio) than the control group during the neonatal period. Conclusion The probiotics oral administration during the third trimester may decreased the relative abundance of Bacteroidetes of their 6 months infants gut microbiota, and increased the Firmicutes to Bacteroidetes ratio. But the effect on the diversity of infants gut microbiota was limited. diversity offspring' Gastrointestinal Microbiome pregnancy probiotics species differences Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3989775","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":276738797,"identity":"f95e380a-67f3-465c-9e33-bd0304038212","order_by":0,"name":"Yongchuang Lin","email":"","orcid":"","institution":"the First Affiliated Hospital of Jinan University(Guangdong)","correspondingAuthor":false,"prefix":"","firstName":"Yongchuang","middleName":"","lastName":"Lin","suffix":""},{"id":276738798,"identity":"ed129ed8-20a4-456e-82a7-30b1244c1b3b","order_by":1,"name":"Yang 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03:49:29","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3989775/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3989775/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":55541759,"identity":"bfbcc773-55d4-42ac-9630-8c1c3b9270ba","added_by":"auto","created_at":"2024-04-29 17:39:24","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1143291,"visible":true,"origin":"","legend":"","description":"","filename":"BMCManuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3989775/v1_covered_6ed9bf41-380b-43c7-8de5-42b14d62cacb.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"The effect of probiotics oral administration during gestation on infant gut microbiota in early 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differences","lastPublishedDoi":"10.21203/rs.3.rs-3989775/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3989775/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eProbiotics have been widely used by pregnant women, but the effect of probiotics on the infant gut microbiota has not been clearly confirmed.This study aimed at investigating the effects of probiotics oral administration during the third trimester on infants' gut microbiota within the first 6 months following vaginal delivery.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eHealthy Chinese pregnant women were recruited and randomly divided into the probiotic group and the control group. The first-pass meconium samples and the fecal samples of infants were collected by trained personnel on 3 days, 14 days, 6 months following vaginal birth. The 16SrRNA gene sequencing technology was applied to analyze the infants' gut microbiota.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003e1. Firmicutes and Proteobacteria were the most dominant phyla in both control and probiotic groups within the first 6 months of life. 2. The diversity of the infants gut microbiota had no difference between the two groups at any time points during the first 6 months of life. 3. There was a lower relative abundance of Bacteroidetes of the probiotic group infants compared with that of the control group infants on 0 day, 14 days as well as 6 months. 4. The probiotic group infants displayed a higher Firmicutes to Bacteroidetes ratio(F/B ratio) than the control group during the neonatal period.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eThe probiotics oral administration during the third trimester may decreased the relative abundance of Bacteroidetes of their 6 months infants gut microbiota, and increased the Firmicutes to Bacteroidetes ratio. 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