Synthesis of novel anti-biofilm agent TiO2@GO for reducing the formation of Streptococcus mitis biofilm on teeth

Synthesis of novel anti-biofilm agent TiO2@GO for reducing the formation of Streptococcus mitis biofilm on teeth | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Synthesis of novel anti-biofilm agent TiO2@GO for reducing the formation of Streptococcus mitis biofilm on teeth Md. Romzan Ali, Md. Sakib Hassan, Dipto Debnath Ridoy, Prity Lata Mozumder, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4121405/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Treatment of global infectious diseases is becoming trickier due to the biofilm formation of bacteria. A novel therapeutic approach is urgently required to address the problems posed by pathogens' persistent infections linked with biofilms. Oral biofilm caused by Streptococcus mitis may generate several dental health issues such as dental caries and periodontal disease. Here, a novel nanomaterial (TiO2@GO) had proposed as an anti-biofilm agent to hinder the formation of biofilm of S. mitis. TiO2 was synthesized by following the sol-gel method and attached with GO through the Teflon line reactor-assisted method. The interaction of TiO2@GO was confirmed through scanning electron microscopy, Fourier transforms infra-ray spectroscopy, and UV-vis spectroscopy analysis. The inhibition of biofilm was confirmed by measuring the OD of S. mitis after and before treatment through an ELISA reader and performing SEM of teeth after and before treatment. The TiO2@GO inhibited the rate of biofilm formation (89% of biofilm was reduced after 8h of treatment). The TiO2@GO can reduce 70% biofilm formation for 24h which is effective to prevent dental caries. To conclude, the proposed nanomaterial will be the potential agent to inhibit the formation of oral biofilm and treat dental caries. Biological sciences/Biotechnology Biological sciences/Drug discovery Biological sciences/Microbiology Health sciences/Health care Physical sciences/Nanoscience and technology TiO2@GO biofilm inhibition Streptococcus mitis dental health and anti-biofilm agent Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarydataandinformation.docx GraphicalAbstractdental.tif Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4121405","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":289327359,"identity":"8abd234b-1364-4397-aaea-172621ae992c","order_by":0,"name":"Md. 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