The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis

Tuba Özcan Metin, Nafiye Yılmaz, Banu Coşkun Yılmaz, Necat Yılmaz, Mehmet Emin Erdal, İlay Buran Kavuran, Gülhan Örekici Temel, Adem Doğaner
In: Cukurova Medical Journal · 2023 · vol. 48(3) , pp. 815–824 · doi:10.17826/cumj.1310778 · W4387011331
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Endometrial TRPV6 and PMCA1 protein expression were elevated in infertile women with unexplained infertility or endometriosis, while TRPV6 mRNA and calcium levels were higher in unexplained infertility patients compared to controls.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 · read from full text ⓘ

This study examined TRPV6 and PMCA1 expression in mid-secretory endometrium from infertile women with unexplained infertility (UI) and/or endometriosis (E), using immunohistochemistry and real-time PCR, alongside calcium quantification by ICP-MS, compared with healthy fertile controls. TRPV6 and PMCA1 protein expression were significantly increased in both luminal and glandular epithelium in the UI and E groups versus controls, while TRPV6 mRNA was significantly lower in the UI group and PMCA1 mRNA did not differ significantly across groups; women with UI also had significantly higher endometrial calcium concentrations. The paper does not provide detailed limitations in the abstract, but it frames the altered expression and calcium differences as potential underlying contributors to infertility requiring further functional studies. Relevance to endometriosis: endometriosis is directly studied in the UI/E comparison, with TRPV6 and PMCA1 protein upregulation reported in the endometriosis group during the mid-secretory window of implantation.

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Abstract

Purpose: The goal of this study was to evaluate the expression of transient receptor potential vanilloid 6 (TRPV6) and plasma membrane Ca2+ ATPase 1 (PMCA1) in the endometrium of infertile women with unexplained infertility (UI) and endometriosis (E), and to compare the findings to healthy fertile women. Materials and Methods: Endometrial expression of TRPV6 and PMCA1 proteins was evaluated during the mid-secretory phase using the immunohistochemical method, and mRNA levels were measured by real-time PCR. Calcium concentrations were determined using Inductively coupled plasma-mass spectrometry (ICP-MS). Results: TRPV6 and PMCA1 protein expression were significantly increased in both luminal and glandular epithelium of UI or E groups compared to the control group. TRPV6 mRNA expression levels were significantly lower in the UI group (0.525 ± 0.087) compared to the control group (0.809 ± 0.073). There was no significant difference in PMCA1 mRNA expression levels between the groups. Calcium concentration was significantly higher in women with UI (median (Q1-Q3): 50.57 [40.67-51.37], compared to the control group (median (Q1-Q3): 24.29 [20.11-25.55]). Conclusion: The present findings demonstrate altered expressions of TRPV6 and PMCA1 mRNA and protein, along with differences in calcium concentration among patients, especially those with the UI during the window of implantation. These differences might potentially be considered as one of the underlying causes of infertility. Further studies are needed to elucidate the functional roles of these channels and to develop new strategies for pharmaceutical intervention in the treatment of infertility.
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Abstract

Purpose: The goal of this study was to evaluate the expression of transient receptor potential vanilloid 6 (TRPV6) and plasma membrane Ca2+ ATPase 1 (PMCA1) in the endometrium of infertile women with unexplained infertility (UI) and endometriosis (E), and to compare the findings to healthy fertile women.

Materials and methods

Endometrial expression of TRPV6 and PMCA1 proteins was evaluated during the mid-secretory phase using the immunohistochemical method, and mRNA levels were measured by real-time PCR. Calcium concentrations were determined using Inductively coupled plasma-mass spectrometry (ICP-MS).

Results

TRPV6 and PMCA1 protein expression were significantly increased in both luminal and glandular epithelium of UI or E groups compared to the control group. TRPV6 mRNA expression levels were significantly lower in the UI group (0.525 ± 0.087) compared to the control group (0.809 ± 0.073). There was no significant difference in PMCA1 mRNA expression levels between the groups. Calcium concentration was significantly higher in women with UI (median (Q1-Q3): 50.57 [40.67-51.37], compared to the control group (median (Q1-Q3): 24.29 [20.11-25.55]).

Conclusion

The present findings demonstrate altered expressions of TRPV6 and PMCA1 mRNA and protein, along with differences in calcium concentration among patients, especially those with the UI during the window of implantation. These differences might potentially be considered as one of the underlying causes of infertility. Further studies are needed to elucidate the functional roles of these channels and to develop new strategies for pharmaceutical intervention in the treatment of infertility.

Keywords

Calcium metabolism, endometriosis, TRPV6, PMCA1, unexplained infertility Açıklanamayan infertilite ve endometriozisli infertil hastaların mid-sekretuar endometriyumunda TRPV6 ve PMCA1’in ekspresyonu

Abstract

Amaç: Bu çalışmanın amacı, açıklanamayan infertilite (Aİ) ve endometriozisi (E) olan infertil kadınların endometriyumunda Transient Reseptör Potansiyel Vanilloid 6 (TRPV6) ve Plazma Membran Ca2+ ATPaz 1 (PMCA1) ekspresyonunu değerlendirmek ve bulguları sağlıklı fertil kadınlarla karşılaştırmaktır. Gereç ve Yöntem: TRPV6 ve PMCA1 proteinlerinin endometriyal ekspresyonu mid-sekretuar faz sırasında, immünohistokimyasal yöntemle değerlendirilmiş ve mRNA seviyeleri real-time PCR ile ölçülmüştür. Kalsiyum konsantrasyonları İndüktif Eşleşmiş Plazma-Kütle Spektrometrisi (ICP-MS) kullanılarak belirlenmiştir. Bulgular: TRPV6 ve PMCA1 protein ekspresyonu kontrol grubu ile karşılaştırıldığında, Aİ ve E gruplarının hem luminal hem de glandular epitelinde anlamlı olarak artış saptanmıştır. TRPV6 mRNA ekspresyon seviyeleri Aİ grubunda (0.525 ± 0.087) kontrol grubuna (0.809 ± 0.073) kıyasla anlamlı derecede düşüktür. PMCA1 mRNA ekspresyon düzeylerinde gruplar arasında anlamlı bir fark yoktur. Kalsiyum konsantrasyonu Aİ’li kadınlarda (medyan (Q1-Q3): 50.57 [40.67-51.37], kontrol grubuna (medyan (Q1-Q3): 24.29 [20.11-25.55]) göre anlamlı derecede yüksek bulunmuştur. Sonuç: Mevcut bulgular, implantasyon penceresi sırasında özellikle Aİ’li hastalarda, kalsiyum konsantrasyonlarındaki farklılıkların yanı sıra, TRPV6 ve PMCA1 mRNA ve proteinlerinin değişen ekspresyonlarını göstermektedir. Bu farklılıklar, potansiyel olarak infertilitenin altında yatan nedenlerden biri olarak düşünülebilir. Bu kanalların fonksiyonel rollerini aydınlatmak ve infertilite tedavisinde farmasötik yaklaşımda yeni stratejiler geliştirmek için daha fazla çalışmaya ihtiyaç vardır.

Keywords

Kalsiyum metabolizması, endometriozis, TRPV6, PMCA1, açıklanamayan infertilite

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endometriosisinfertility

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