The study of Overexpression of Peroxiredoxin-2 Reduces MPP+-Induced Toxicity in SH-SY5Y Neuroblastoma Cells  

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by dopaminergic neuron loss. Excessive reactive oxygen species (ROS) accumulation has been implicated in the pathogenesis of Parkinson’s disease. Endogenous Peroxiredoxin-2 (Prdx-2) has potent anti-oxidative and anti-apoptotic effects. For further study of the activation of Prdx-2 and its role in vitro, SH-SY5Y cells were treated with MPP + or transfected with Prdx-2. The results showed that MPP + raises intracellular ROS, depolarizes mitochondrial membrane potential, decreases cell activity, and reduces expression of tyrosine hydroxylase (TH), Prdx-2, Silent Information Regulator of Transcription 1 (SIRT1), and increases the proapoptotic/anti-apoptotic expression ratio (Bax/Bcl2). When cells were transfected with Prdx-2, TH and SIRT1 were increased, the Bax/Bcl-2 ratio was decreased, ROS content was decreased, and cell activity was enhanced. However, this study not directly test whether Prdx-2 affects the downstream signaling factors of SIRT1.This will be the focus of the next stages of research.