Acrocomia aculeata oil-loaded nanoemulsion: development, stability, antinflammatory properties and cytotoxicity evaluation

chemistry Medicinal and pharmaceutical chemistry Nano- and molecular-scale electronics Nano-biomaterials and bioscience Nanomagnetics Nanomaterials, thin films and nanointerfaces Nanomedicine Nanometrology and nanomechanics Nano-optics Nanopatterning, self-assembly and nanofabrication Nanostructures for energy and sensing applications Natural products chemistry Organo main group chemistry Other nanotechnology (unclassified) Other organic chemistry (unclassified) Photochemistry and photovoltaics Physical organic chemistry Supramolecular chemistry The oil from the pulp of the bocaiúva fruit may have several medical applications. However, little is known about its pharmacological activity. Therefore, this study aimed to develop and evaluate the anti-inflammatory activity of a nanoemulsion loaded with the oil extracted from the pulp of the fruit of Acrocomia aculeata. Griffin's method determined the hydrophilic-lipophilic equilibrium ratio of the nanoemulsion. It was shown to have an adequate particle size (173.60 nm) with excellent homogeneity (polydispersity index 0.200). The anti-inflammatory activity of the nanoemulsion was evaluated by the carrageenan-induced paw edema method. Finally, the hemolytic and cytotoxic activity of the nano formulation were determined to assess its safety. This nanoemulsion loaded with Acrocomia aculeata fruit pulp oil was shown to have parameters suitable to its characterization, impressive anti-inflammatory activity, and a safe profile.

Acrocomia aculeata; inflammation; nanoemulsion; cytotoxicity; hemolysis When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information: Bautista-Robles, V.; Keita, H.; Paredes Gamero, E. J.; Brito Leite, L. T.; de Araújo Isaias Muller, J.; Toffoli Kadri, M. C.; Lafourcade Prada, A.; Rodríguez Amado, J. R. Beilstein Arch. 2025, 202518. doi:10.3762/bxiv.2025.18.v1 Citation data can be downloaded as file using the "Download" button or used for copy/paste from the text window below. Citation data in RIS format can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Zotero. © 2025 Bautista-Robles et al.; licensee Beilstein-Institut. This is an open access work licensed under the terms of the Beilstein-Institut Open Access License Agreement (https://www.beilstein-archives.org/xiv/terms), which is identical to the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0). The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this work could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material.