Generation of a novel recombinant antibody for sensitive detection of Pseudomonas aeruginosa

Pseudomonas aeruginosa ( P. aeruginosa ) is a major pathogen that causes nosocomial infections and often exhibits antibiotic resistance. Therefore, the development of an accurate method for detecting P. aeruginosa is required to control P. aeruginosa -related outbreaks. In this study, we established an enzyme-linked immunosorbent assay (ELISA) method for the sensitive detection of three P. aeruginosa strains, UCBPP PA14, ATCC 27853, and multidrug-resistant ATCC BAA-2108. We produced a recombinant antibody (rAb) against P. aeruginosa using mammalian cells with high yield and purity, and confirmed its P. aeruginosa binding efficiency. The rAb was paired with commercial anti- P. aeruginosa Ab for a sandwich ELISA, resulting in an antigen-concentration- dependent response with a limit of detection value of 23 CFU. These results suggest that the rAb produced herein can be used for the sensitive detection of P. aeruginosa with a wide range of applications in clinical diagnosis and point-of-care testing.