Expression Analysis of miR-519a-3p and miR-379-5p in Colorectal Cancer Patients: A Combined Experimental and Bioinformatic Approach.

A total of 54 patients with histopathological confirmed CRC (31 males and 23 females; 33 with colon cancer and 21 with rectal cancer) who were admitted to Gaziantep University Hospital between 2017 and 2020 were enrolled in this study. Paired tumor and adjacent non-tumor colorectal tissue specimens were obtained from each patient. Inclusion criteria comprised a confirmed diagnosis of colorectal adenocarcinoma and the absence of any previous chemotherapy or radiotherapy. Exclusion criteria included a history of other malignancies, autoimmune or inflammatory diseases, cardiovascular diseases within the past six months, active infections, long-term immobilization, and inability to undergo surgical intervention. Tissue samples were quickly frozen in liquid nitrogen and stored in RNAlater ® solution (Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA) at −80 °C. Ethical approval for this research was granted by the Gaziantep University Ethics Committee, Turkey (Approval No. 2020/114). All participants provided written informed consent prior to inclusion in the study. The research procedures adhered to the ethical standards outlined in the Declaration of Helsinki. Tissue specimens obtained from CRC patients were preserved in RNAlater ® solution and kept at −80 °C. Prior to miRNA extraction, the tissues were thoroughly homogenized. Total RNA, including miRNAs, was extracted from the tumor samples using the mirVana™ miRNA Isolation Kit with phenol (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, AM1560) following the manufacturer’s guidelines. RNA concentration and purity were assessed via a NanoDrop spectrophotometer (Maestrogen, Hsinchu, Taiwan), while RNA integrity was confirmed by electrophoresis on a 1% agarose gel. All isolated RNA samples were subsequently stored at −80 °C. cDNA was generated using the Reverse Transcriptase PCR (RT-PCR) technique with the TaqMan™ Advanced miRNA cDNA Synthesis Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA, A28007 ). The resulting cDNA products were preserved at −20 °C until analysis with Quantitative Real-Time PCR (RT-qPCR). The Quantitative Real-time PCR (RT-qPCR) was performed using StepOne & StepOnePlus Real-Time PCR Systems (Applied Biosystems, USA) to assess expression levels of miR-379-5p, miR-519a-3p, and RNU6B as housekeeping gene. TaqManTM Advanced miRNA Assay (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA, A25576), TaqManTM Primer Probe (Thermo Fisher Scientific, Waltham, MA, USA) and TaqManTM Fast Advanced Master Mix (Thermo Fisher Scientific, 4444557, Applied Biosystems, USA) were used for RT-qPCR [ 23 ]. The sequences of primers are: miR-379-5p: Forward 5′-TGGTAGACTATGGAACGTAGG-3′, Reverse 5′-CGAGGAAGAAGACGGAAGAAT, miR-519a-3p: Forward 5′-AAAGTGCATCCTTTTAGAGTGT-3′, Reverse: 5′-CGAGGAAGAAGACGGAAGAAT-3′; RNU6B : Forward 5′-GCTTCGGCAGCACATATACTAAAAT-3′; Revers 5′-CGCTTCACGAATTT GCGTGTCAT-3′. Each sample was analyzed in triplicate. The relative expression levels of miR-379-5p, miR-519a-3p were determined using the 2 −ΔΔCt method [ 24 ]. All variables were expressed as mean ± standard deviation (SD) and analyzed using SPSS (version 22.0.0.0 SPSS, Inc., Chicago, IL, USA). Differences in miRNA expression between tumor tissues and adjacent non-tumor tissues were assessed by calculating ΔCt for each group and applying the 2 −ΔΔCt approach. The normality of numerical variables was evaluated with both the Kolmogorov–Smirnov and Shapiro–Wilk tests. Since Ct values followed a normal distribution, paired t -tests were used to compare miR-379-5p and miR-519a-3p expression between tumor and non-tumor samples. For fold change values, which were not normally distributed, the non-parametric Mann–Whitney U test was employed to analyze associations with clinicopathological characteristics. Statistical significance was set at p < 0.05. Bioinformatics utilizes computational methods to store, retrieve, and analyze biological data, enabling insights into disease mechanisms and gene regulation. It provides a broad range of techniques, including database construction, gene discovery, and data clustering, which are used in cancer research and the investigation of other diseases [ 25 ]. The Kyoto Encyclopedia of Genes and Genomes (KEGG) provides extensive data on gene regulatory pathways, along with a visualization tool. As a result, KEGG has become the main source of information for modeling and simulating biological systems and networks [ 26 ]. To explore downstream biological pathways, miRNA signatures were analyzed via the DIANA-miRPath v3.0 online platform using KEGG and GO databases. GO enrichment was assessed across three levels: biological processes (BP), cellular components (CC), and molecular functions (MF). Target genes for the included miRNAs were identified through the TarBase/microT-CDs algorithm and subsequently used for functional enrichment analysis. A threshold of p < 0.05 was considered statistically significant. To elucidate regulatory interactions between miR-379-5p, miR-519a-3p, and transcription factors (TFs), the study utilized the TransmiR v3.0 database ( http://www.cuilab.cn/transmir (accessed on 5 June 2025)), which provides information on experimentally validated regulatory relationships between miRNAs and TFs and between TFs and TFs [ 27 ]. To further explore the downstream regulatory effects of TFs, version 2.0 of the TRRUST database (Transcriptional Regulatory Relationships Unravelled by Sentence-based Text-mining) was employed ( https://www.grnpedia.org/trrust/ (accessed on 5 June 2025)) [ 28 ]. TRRUST contains curated TF–target gene interactions extracted from the literature using a text-mining approach. It also includes associated disease annotations for TFs, which allowed us to map TFs to potential disease phenotypes. Additionally, the functional characterization of TFs was performed using data from The Human Protein Atlas ( https://www.proteinatlas.org (accessed on 5 June 2025)) [ 29 ], which provides functional annotations based on biological pathways and molecular functions.

Colorectal cancer (CRC) is a diverse malignancy arising from the colon or rectum and represents one of the leading cancer types globally. Based on 2024 epidemiological data, CRC holds the third position among the most commonly diagnosed cancers in both sexes. It is reported as the third leading cause of cancer-related mortality in men and the fourth in women [ 1 ]. CRC formation is a multi-step process driven by a number of genetic and epigenetic processes that lead to the progression from normal mucosal polyps to carcinoma. Together, these events enable cells to bypass many normal regulatory mechanisms, resulting in malignant characteristics such as uncontrolled proliferation, resistance to apoptosis, and tissue invasion [ 2 ]. Various environmental and genetic factors contribute to CRC pathogenesis. These include age, sex, alcohol and tobacco use, obesity, dietary habits, physical inactivity, and underlying chronic diseases such as diabetes mellitus, inflammatory bowel disease, and pre-existing colon polyps [ 3 ]. microRNAs (miRNAs) are short, single-stranded, non-coding RNA sequences of about 22 nucleotides that play an essential role in modulating gene expression by binding to the 3′ untranslated regions (3′UTRs) of their target transcripts [ 4 , 5 ]. The 3′UTR of a gene can contain multiple binding sites for a wide variety miRNA molecule. Conversely, one miRNA molecule has the capacity to interact with the 3′UTRs of multiple target transcripts. Through this mechanism, numerous miRNAs can finely tune gene expression, and a single miRNA can simultaneously co-regulate several genes [ 6 ]. miRNAs are widely distributed across eukaryotic organisms and some of the miRNAs are associated with various types of cancer [ 7 ]. miRNAs are involved in many biological pathways associated with cancer, including uncontrolled cell proliferation, angiogenesis, invasion, metastasis, and apoptosis. Many research studies have reported that miRNAs can increase malignant formation by suppressing tumor suppressor genes or increasing oncogene expression and miRNAs can function as clinical markers in various carcinogenesis mechanism [ 5 , 8 ]. Recent studies have demonstrated that the expression levels of miR-2861 and miR-5011-5p are significantly reduced in CRC tissues [ 9 ]. miR-564 and miR-718 downregulation in colorectal tumor tissues were associated with tumor suppressor functions [ 10 ]. In another study, miR-17-5p was upregulated in CRC tissues compared to adjacent normal tissues [ 11 ]. Therefore, miRNAs are increasingly recognized as key regulators of tumor biology. Multiple investigations have reported that miR-379-5p, mapped to chromosome 14q32.31, exhibits variable expression patterns across a range of malignancies, including endometrial cancer [ 12 ], glioma [ 8 ], ovarian [ 13 ], oral squamous cell carcinoma [ 14 ], bladder [ 15 ] and hepatocellular carcinoma [ 16 ]. miR-519a-3p, which is located on chromosome 14, was dysregulated in various diseases, such as gastric cancer [ 17 ], breast cancer [ 18 , 19 ], neuroblastoma [ 20 ], Alzheimer’s disease [ 21 ], and Parkinson’s disease [ 22 ]. As a result of our preliminary study using bioinformatics analysis tools, we determined that some of the genes targeted by miR-379-5p and miR-519a-3p play a role in colorectal carcinogenesis. To the best of our knowledge, the association between miR-379-5p and miR-519a-3p and CRC has not yet been clearly established. Moreover, the specific target genes of miR-379-5p and miR-519a-3p, along with their involvement in signaling pathways and biological processes, remain undefined. Additionally, the target transcription factors (TFs) of miR-379-5p and miR-519a-3p, the genes regulated by these TFs, and their association with related diseases have not been demonstrated. Therefore, the present study aims to investigate the functional roles of miR-379-5p and miR-519a-3p in CRC.

This retrospective analysis included 54 patients diagnosed with CRC, of whom 23 were women. In 30 tumor specimens, the largest dimension measured ≤6 cm. Based on TNM classification, 29 patients were categorized as Stage I–II and 25 as Stage III–IV. Comprehensive demographic, clinical, and pathological characteristics are summarized in Table 1 . Analysis revealed a mean fold change of 1.57 ± 1.06 for miR-379-5p, which was significantly upregulated in tumor tissues compared with adjacent non-tumor samples ( p = 0.004) ( Figure 1 A). In contrast, miR-519a-3p exhibited a pronounced downregulation, with a mean fold change of 0.50 ± 0.11 in tumor tissues relative to adjacent controls ( p < 0.001) ( Figure 1 B). The upregulated expression of miR-379-5p was associated with CRC patients age ≥55 years ( p = 0.031), tumor location in the rectum ( p = 0.033), advanced clinical TNM stage (Stage III–IV) ( p = 0.003), and tumor size ≤ 6 cm ( p = 0.012). However, no statistically significant associations were observed between miR-379-5p expression and gender ( p = 0.643), perineural invasion ( p = 0.316), lymphovascular invasion ( p = 0.708), or histological tumor type ( p = 0.061). A statistically significant relationship was found between the downregulated expression of miR-519a-3p and the localization of the tumor in the rectum ( p = 0.01), but no significant association was observed between other characteristics of the patients ( p > 0.05). The detailed data are presented in Table 2 . GO enrichment analysis was performed using the DIANA-miRPath database to functionally characterize the roles of miR-379-5p and miR-519a-3p. The enriched GO terms were categorized under three main domains: Biological Process (BP), Cellular Component (CC), and Molecular Function (MF), as summarized in Table 3 . In the BP category, the most significantly enriched terms included: Cellular nitrogen compound metabolic process (GO:0034641), Cellular protein metabolic process (GO:0044267), Biosynthetic process (GO:0009058), Response to stress (GO:0006950). In the CC category, the top enriched terms were: Organelle (GO:0043226), Protein complex (GO:0043234), Cytosol (GO:0005829), Nucleoplasm (GO:0005654). In the MF category, the most enriched terms included: Enzyme binding (GO:0019899), Ion binding (GO:0043167), Nucleic acid binding transcription factor activity (GO:0001071). KEGG pathway enrichment analysis of miRNAs performed using the DIANA-miRPath database revealed significant enrichment of 13 pathways listed in Table 4 , including “p53 signaling pathway (hsa04115),” “Endocytosis (hsa04144),” “TGF-beta signaling pathway (hsa04350),” and “FoxO signaling pathway (hsa04068),” which are particularly relevant to colorectal carcinogenesis ( Supplementary Table S1 ). In addition to bioinformatic analyses, the target genes of both miR-379-5p and miR-519a-3p, which play a role in CRC formation, were identified and shown in Figure 2 . It was observed that miR-379-5p targets CASP3 , while miR-519a-3p targets APC and AKT3 . The gene products of CASP3 , AKT3 , and APC play important roles in the ERK, PI3K, RAS, WNT, and TGFB signaling pathways and apoptosis, which are involved in the development of CRC. TFs play important roles in regulating the transcription of miRNAs. In the present study, comprehensive regulatory networks between TFs and miR-379-5p and miR-519a-3p were analyzed using the TransmiR v3.0 database. The resulting TF–miRNA regulatory network is illustrated in Figure 3 . The analysis revealed that miR-519a-3p interacted with 6 TFs and miR-379-5p interacted with 73 TFs. As a result of the analysis performed using the TransmiR v3.0 online tool, 2 common TFs (Estrogen Receptor 1 (ESR1) and Forkhead box protein A1 (FOXA1)) interacting with both miRNAs were detected. Furthermore, it was determined that miR-519a-3p is effective in the regulation of KLF transcription factor 4 (KLF4) and Progesterone receptor (PGR), while miR-379-5p is effective in the regulation of Signal transducer and activator of transcription 1 (STAT1), SMAD family member 2 (SMAD2), Hepatocyte nuclear factor 4 alpha (HNF4A), MYC associated zinc finger protein (MAZ), Jun protooncogene (JUN), Activating transcription factor 1 (ATF1), and E1A binding protein p300 (EP300). As TFs play a critical role in the regulation of miRNA expression, and because miRNAs exert their functions by targeting specific genes, TFs interacting with miRNAs, as well as diseases associated with the genes regulated by these TFs, were identified using the TRRUST database. A summary of these findings can be found in Table 5 . As shown in Table 5 , these TFs were found to function in important biological processes such as proliferation, differentiation, and embryonic development. Additionally, it was revealed that TFs are associated with numerous types of cancer, including CRC [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ].

CRC represents a significant global public health concern and continues to rank among the most prevalent malignancies worldwide. In recent years, accumulating evidence has demonstrated a strong association between miRNAs and the development and progression of multiple human cancers. These small non-coding RNAs have attracted increasing attention from researchers due to their important roles in cell differentiation, biological development, and the formation and progression of diseases, including cancer. However, the factors affecting miRNAs in this process, and the reasons behind their varying expression in different tumor types, are still not clearly understood. This study compared the expression profiles of miR-379-5p and miR-519a-3p between tumor and adjacent normal tissues in patients with CRC and conducted bioinformatic analyses for these microRNAs. The findings revealed a significant upregulation of miR-379-5p in tumor samples compared to corresponding normal colon or rectal tissues. A review of previous studies indicated that the regulation of miR-379-5p varies with cancer type, showing either downregulation or upregulation. For example, elevated miR-379 expression has been reported to enhance tumor proliferation and facilitate bone metastasis in prostate cancer tissues and cell lines. The same study also found an association between miR-379 expression and the progression-free survival of prostate cancer patients [ 41 ]. Unlike the results of our study, several prior investigations have reported that miR-379-5p is downregulated in different cancer types and linked to tumor development. Liang et al. demonstrated that miR-379-5p inhibited the growth, migration, and invasion of endometrial cancer cells [ 12 ], while Shukla et al. observed its downregulation in ovarian cancer, both in cell lines and patient-derived tumor specimens [ 13 ]. Another study revealed that miR-379-5p was downregulated in serum samples of oral squamous cell carcinoma patients [ 14 ]. Moreover, Mosaad et al. discovered that miR-379-5p expression levels was significantly decreased in endometrial cancer tissues by targeted Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) [ 42 ]. Zhang et al. showed that miR-379-5p increased the proliferation of articular chondrocytes in osteoarthritis patients by regulating the PI3K/AKT pathway [ 43 ]. In addition to these studies in the literature, miR-379-5p was also found to be down-regulated in lung adenocarcinoma [ 44 ], glioblastoma [ 45 ] and hepatocellular carcinoma [ 16 ]. In addition, we analyzed the expression of miR-519a-3p in CRC and observed a reduction in its levels in tumor tissues relative to adjacent normal tissues. Consistent with these findings, Gu et al. reported that miR-519a-3p expression was also suppressed in osteosarcoma cells [ 46 ]. Li et al. found down-regulation of miR-519a and up-regulation of its target gene Signal transducer and activator of transcription 3 ( STAT3 ) in relapsed glioblastoma tissues compared to tissues from patients with primary glioblastoma. The researchers found a significant inverse correlation between miR-519a and STAT3 expression levels [ 47 ]. Likewise, previous studies have indicated that miR-519a acts as a tumor suppressor in non-small cell lung cancer by regulating STAT3 , thereby inhibiting tumor advancement [ 48 ]. Another study reported that miR-519 expression was significantly reduced in pancreatic cancer cell lines in a hypoxic environment, indicating that miR-519 may play a suppressive role in hypoxia-induced oncogenic phenotypes of pancreatic cancer [ 49 ]. In contrast to the results obtained from our study, miR-519a-3p expression level in the serum exosomes of gastric cancer patients with liver metastases was found to be higher than that of gastric cancer patients without metastasis and a correlation was found between high exo-miR-519a-3p expression and poor prognosis [ 17 ]. Ward et al. (2014) identified miR-519a as a novel oncomir that regulates the tumor suppressor gene network in breast cancer and leads to resistance to tamoxifen [ 50 ]. Moreover, miR-519a-3p has been identified as playing a pivotal role in inhibiting apoptosis of breast cancer cells and reducing their detection by natural killer (NK) cells [ 18 ]. In the present study, GO and KEGG pathway analyses were conducted to identify the signaling pathways linked to altered miRNAs, as well as the biological processes, cellular components, and molecular functions related to their target genes. The GO analysis demonstrated that miR-379-5p and miR-519a-3p were associated with 114 genes in biological processes, 113 genes in cellular components, and 115 genes in molecular functions, respectively. KEGG pathway analysis exhibited that dysregulated miRNAs were associated with signaling pathways such as p53, Transforming growth factor beta (TGF-β), and FoxO signaling pathways, which have important functions in carcinogenesis. In support of our findings, many researchers have previously demonstrated that p53, TGF-β and FoxO signaling pathways are effective in CRC formation and progression. An important tumor suppressor, p53 is an important transcription factor that regulates various cellular responses to prevent the transformation of a normal cell into a cancer cell. In the literature, it is reported that p53 mutations are present in 43% of all CRCs [ 51 ]. The TGF-β pathway is essential for numerous key biological functions, such as cell differentiation, proliferation, growth, programmed cell death (apoptosis), epithelial–mesenchymal transition (EMT), remodeling of the extracellular matrix (ECM), and angiogenesis. Alterations in TGF-β signaling are effective in many cancer types, including CRC [ 52 ]. The Forkhead box (FOX) family of transcription factors has multiple important roles during human development. FOX gene group transcriptional defects in this pathway, which is associated with numerous molecular signaling pathways, have been associated with several types of human cancer. Alterations in at least 14 FOX gene groups have been reported to be associated with CRC formation [ 53 ]. In the current study, it was also demonstrated that CASP3 , APC , and AKT3 , which play important roles in colorectal carcinogenesis, are targeted by miR-379-5p ( CASP3 ) and miR-519a-3p ( APC and AKT3 ) ( Figure 2 ). CASP3, an important mediator of apoptosis, functions as an important component of the cell death mechanism as a result of cells being exposed to cytotoxic drugs and radiotherapy. However, recent studies have also shown that CASP3, which is used as a marker for the efficacy of cancer treatment, also plays non-apoptotic roles such as tumor recurrence and tumor angiogenesis. Zhou et al. reported that CASP3 regulates the migration, invasion, and metastasis of colon cancer cells [ 54 ]. It is known that somatic mutations in the APC gene play an initiating role in approximately 80% of all CRCs [ 55 ]. Another study exhibited a significant increase in the AKT3 gene in CRC tissues compared to normal tissues [ 56 ]. Gene regulation, which has a dynamic and complex structure, is one of the most important mechanisms of biological processes, and disruption of this mechanism can lead to human diseases. Among the molecules that play an important role in regulating gene expression are TFs and miRNAs. The targets of miRNAs include genes that encode TFs. TFs activate or inhibit transcription by binding to specific regulatory sequences in genes. miRNAs can target various TFs and thus regulate TF expression, thereby influencing tumor development [ 57 , 58 ]. In our study, both miR-379-5p and miR-519a-3p were found to have ESR1 and FOXA1 as common target transcription factors ( Table 5 ). Previous studies have shown a relationship between ESR1, which is encoded by the estrogen receptor 1 gene and plays a role in cellular proliferation and differentiation, and CRC [ 59 ]. FOXA1, which plays a central role in various biological processes such as organogenesis, differentiation, glycolipid metabolism, proliferation, migration, and invasion, has been reported to be highly expressed in normal human colon tissue but downregulated in colon adenocarcinoma [ 60 ]. The information in the literature provides evidence for the relationship between miR-379-5p and miR-519a-3p and CRC. The presence of TFs among the predicted targets of miR-379-5p and miR-519a-3p, many of which are critically involved in essential biological processes such as the regulation of eukaryotic gene expression, cell proliferation and differentiation, embryonic development, intercellular signaling, and chromatin remodeling, further underscores the biological significance of these two miRNAs ( Table 5 ). The expression of miR-379-5p and miR-519a-3p varies markedly among different malignancies. Although the pathways involved in miRNA synthesis are partly understood and substantial progress has been made in this area, the regulatory mechanisms driving miRNA production and their impact on tumor development remain insufficiently clarified. Recent evidence suggests that miRNA dysregulation can result from gene amplification or deletion, altered transcription factor activity, epigenetic modifications, and changes in genes responsible for miRNA processing. Furthermore, competitive endogenous RNAs (ceRNAs) play an important role in modulating miRNA abundance [ 61 ]. In light of the existing data, it is inevitable that the expression levels of the two miRNAs analyzed in our study will either align with or deviate from the findings reported in the literature. Detailed examination of the mechanisms affecting the expression levels of both miR-379-5p and miR-519a-3p in CRC may be the subject of future studies. Our results suggest that miR-379-5p and miR-519a-3p have potential to serve as biomarkers in CRC. Numerous studies have identified various miRNAs, including miR-21, miR-15b, miR-31 and miR-200c as promising biomarkers for the diagnosis or prognosis of CRC [ 62 , 63 , 64 ]. It has been demonstrated that these miRNAs are capable of regulating pivotal signaling pathways that are implicated in processes such as cancer proliferation, invasion, and metastasis. The current study indicates that miR-379-5p and miR-519a-3p may also contribute to colorectal carcinogenesis, thus warranting further investigation as potential diagnostic markers. It is important to consider the different expressions and potential functional importance of these miRNAs, as this could enhance the sensitivity and specificity of multi-miRNA panels when combined with already validated miRNAs. Further studies involving larger patient cohorts and functional validation are needed to investigate their additive or synergistic value in CRC biomarker strategies. In this research, RT-qPCR was used to evaluate miR-379-5p and miR-519a-3p expression in CRC patient tissues, and complementary bioinformatic analyses were conducted to explore their functional significance. Although the study has its strengths, it also has some limitations. Firstly, although the different expressions of miR-379-5p and miR-519a-3p have been clearly demonstrated, the target genes of these miRNAs have not been experimentally investigated. Without directly identifying and validating their targets, it is not entirely accurate to make clear statements about the biological and functional importance of miRNAs based on the changes observed in their expression levels. Future studies that experimentally validate the target genes identified through bioinformatic analyses will be crucial for elucidating the molecular mechanisms by which these miRNAs are involved in CRC initiation and progression. Secondly, the study does not include in vivo and in vitro functional experiments, which limits the ability to determine the direct effects of miR-379-5p and miR-519a-3p on fundamental cellular processes such as proliferation, apoptosis, invasion, and metastasis. In vitro studies using CRC cell lines and in vivo studies using animal models will elucidate the functional roles of these miRNAs and help to validate their potential as diagnostic or therapeutic targets. Ultimately, while this research evaluated the association between miRNA expression profiles and the clinicopathological features of CRC patients, the relatively small cohort size may have restricted both the statistical strength and the broader applicability of the findings. These findings need to be validated in different patient populations and clinical subgroups. In conclusion, while this study provides important preliminary evidence for the dysregulation of miR-379-5p and miR-519a-3p in CRC, further research involving experimental target gene analysis, in vivo and in vitro functional analyses, and larger patient groups is needed to fully understand their roles in the pathogenesis of CRC and their potential clinical significance.

In this study, a marked increase in miR-379-5p expression and a significant reduction in miR-519a-3p levels were observed in CRC tissues relative to the surrounding normal mucosa in patients with CRC. GO analysis, conducted within the scope of bioinformatic analysis, revealed enrichment in several significant biological processes, including biosynthetic processes, cellular protein metabolic processes, stress response, and nucleic acid-binding transcription factor activity. KEGG analysis demonstrated that dysregulated miRNAs are associated with significant pathways related to colorectal carcinogenesis, including p53 signaling, TGF-beta signaling, and FoxO signaling. Concurrently, the analysis of the network comprising miRNAs, TFs, and genes revealed that ESR1 and FOXA1 are common target TFs of dysregulated miRNAs. Network analyses revealed that dysregulated miRNAs interact with CASP3 , APC , and AKT3 in CRC formation pathways. As a result, these findings suggest that miR-379-5p expression is increased and miR-519a-3p is decreased in CRC tissues; this may indicate the potential oncogenic and tumor suppressor tendencies of these miRNAs, respectively. However, functional validation analyses are required to determine their specific roles. Findings from this research indicate that miR-379-5p and miR-519a-3p may serve as promising biomarker candidates for CRC. Additional studies are warranted to clarify the molecular pathways involved in CRC development.