Antibacterial activity and wound healing promotion of biomass carbon dots derived from a forestry pest, Glenea cantor (Coleoptera: Cerambycidae)

Antibacterial activity and wound healing promotion of biomass carbon dots derived from a forestry pest, Glenea cantor (Coleoptera: Cerambycidae) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antibacterial activity and wound healing promotion of biomass carbon dots derived from a forestry pest, Glenea cantor (Coleoptera: Cerambycidae) Gang Ren, Wen Zhang, Yuan Tang, Bangyu Zhong, Liangshan Ming, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6747273/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 12 You are reading this latest preprint version Abstract Currently, forestry is facing ecological challenges caused by pests, highlighting the potential for research into the resource utilization of these pests, which could open new pathways for the sustainable use of biological resources, thereby achieving a win-win situation for both ecology and economy. Despite the recognized potential, the utilization rate of insect resources remains low due to limited research methodologies. Longhorn beetles, as commonly used traditional medicinal insects, possess notable hemostatic properties. This study leverages the hemostatic advantages of carbon dots (CDs) materials, selecting the multivoltine forestry pest Glenea cantor (GC) as the research subject to prepare CDs materials (GC-CDs) and explore the potential value of this innovative material in promoting wound healing. GC-CDs were synthesized through a simple and environmentally friendly microwave-assisted method. Comprehensive physicochemical characterizations were performed using Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Photoelectron Spectroscopy (XPS). The results revealed that GC-CDs exhibit a quasi-spherical structure, excellent water solubility, and an average particle size of 1.02 nm. Furthermore, in vitro cytotoxicity assays, hemolysis tests, and in vivo toxicity evaluations demonstrated the good biocompatibility of GC-CDs. Additionally, GC-CDs effectively inhibited the growth of Escherichia coli ( E. coli ) and exhibited significant wound healing promotion effects in cell scratch assays, Transwell migration tests, and mouse wound models. This study successfully demonstrated the excellent antibacterial properties and wound-healing efficacy of GC-CDs while revealing their potential ecological value in transforming waste into valuable resources. These findings provide an innovative strategy for the eco-friendly utilization of pest resources, such as longhorn beetles. Forestry ecology Pest resource utilization Bioconversion Antibacterial agents Wound repair Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 09 Jun, 2025 Reviews received at journal 06 Jun, 2025 Reviews received at journal 05 Jun, 2025 Reviewers agreed at journal 05 Jun, 2025 Reviews received at journal 01 Jun, 2025 Reviewers agreed at journal 30 May, 2025 Reviewers agreed at journal 30 May, 2025 Reviewers agreed at journal 30 May, 2025 Reviewers invited by journal 30 May, 2025 Editor assigned by journal 29 May, 2025 Submission checks completed at journal 29 May, 2025 First submitted to journal 26 May, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6747273","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":464799116,"identity":"dd2cc535-5db7-4388-913a-5a772cdbdc74","order_by":0,"name":"Gang Ren","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Gang","middleName":"","lastName":"Ren","suffix":""},{"id":464799117,"identity":"abc3ca65-6e9f-4e67-919e-0f0aeeb90d1e","order_by":1,"name":"Wen Zhang","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Wen","middleName":"","lastName":"Zhang","suffix":""},{"id":464799118,"identity":"50a7a2bf-44b8-434a-bc77-561777843cb3","order_by":2,"name":"Yuan Tang","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Yuan","middleName":"","lastName":"Tang","suffix":""},{"id":464799119,"identity":"9fb0e539-6740-47cf-aeb4-710f17c00953","order_by":3,"name":"Bangyu Zhong","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Bangyu","middleName":"","lastName":"Zhong","suffix":""},{"id":464799120,"identity":"29927649-0972-4670-961d-d723226e4113","order_by":4,"name":"Liangshan Ming","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Liangshan","middleName":"","lastName":"Ming","suffix":""},{"id":464799121,"identity":"808f1417-a8a4-412f-9fc6-82d56b8f7ca3","order_by":5,"name":"Qimeng Fan","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Qimeng","middleName":"","lastName":"Fan","suffix":""},{"id":464799122,"identity":"5f02e067-22df-4568-bf92-4092f5434388","order_by":6,"name":"Jia Huang","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Jia","middleName":"","lastName":"Huang","suffix":""},{"id":464799123,"identity":"9a283697-f9af-4333-bc72-21a6a765fb82","order_by":7,"name":"Hongning Liu","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Hongning","middleName":"","lastName":"Liu","suffix":""},{"id":464799124,"identity":"562ec291-fff3-45ec-9215-3700fae95d4c","order_by":8,"name":"Zhixin Li","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Zhixin","middleName":"","lastName":"Li","suffix":""},{"id":464799125,"identity":"e83991d5-2c22-43ee-91ea-60a0e46b597d","order_by":9,"name":"Ping Luo","email":"","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Ping","middleName":"","lastName":"Luo","suffix":""},{"id":464799126,"identity":"41c0ec2a-46f1-4636-9f84-cfddd3838964","order_by":10,"name":"Zishu Dong","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/klEQVRIie3SMUsDMRTA8RcC1yWQNUXwMzw5OByEfpV3FDp1cLyhYKZ0sXSt30IXcXzHwU35AAe33OTkoLjYSVOl4HLXGwXzHzKE/HgJBCAW+4NpKZkJr262wMc9GibTtcu5KxbizgbCYwh6n5adr8T94fgoAg0h507KtPTZ2evqEfRkibB/6hdiRxRIkmRcLwzXLUxvX1BsfD+RhjgQpTKualPaFrBZohSunyQmt4EYk9rSfZPZKaJUBUweEeE4xZwgZuICKYjCO+aXvm6V8c/X5WaAzCr9/vaBn6R3/qIpVu25Xs8fuv0A+TWPf256WHgMCF/HjjsXi8Vi/68vLm9fNL9CznIAAAAASUVORK5CYII=","orcid":"","institution":"Jiangxi University of Chinese Medicine","correspondingAuthor":true,"prefix":"","firstName":"Zishu","middleName":"","lastName":"Dong","suffix":""}],"badges":[],"createdAt":"2025-05-26 05:38:35","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6747273/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6747273/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":83786717,"identity":"a69c5a7c-5e13-4807-bd03-d3907452513e","added_by":"auto","created_at":"2025-06-02 17:19:47","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1582212,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6747273/v1_covered_4a917799-67c2-4bef-9147-85be478223bd.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Antibacterial activity and wound healing promotion of biomass carbon dots derived from a forestry pest, Glenea cantor (Coleoptera: Cerambycidae)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"chemical-and-biological-technologies-in-agriculture","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Chemical and Biological Technologies in Agriculture](https://chembioagro.springeropen.com/)","snPcode":"40538","submissionUrl":"https://submission.nature.com/new-submission/40538/3","title":"Chemical and Biological Technologies in Agriculture","twitterHandle":"@SpringerPlants","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Forestry ecology, Pest resource utilization, Bioconversion, Antibacterial agents, Wound repair","lastPublishedDoi":"10.21203/rs.3.rs-6747273/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6747273/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eCurrently, forestry is facing ecological challenges caused by pests, highlighting the potential for research into the resource utilization of these pests, which could open new pathways for the sustainable use of biological resources, thereby achieving a win-win situation for both ecology and economy. Despite the recognized potential, the utilization rate of insect resources remains low due to limited research methodologies. Longhorn beetles, as commonly used traditional medicinal insects, possess notable hemostatic properties. This study leverages the hemostatic advantages of carbon dots (CDs) materials, selecting the multivoltine forestry pest \u003cem\u003eGlenea cantor\u003c/em\u003e\u003cstrong\u003e \u003c/strong\u003e(GC) as the research subject to prepare CDs materials (GC-CDs) and explore the potential value of this innovative material in promoting wound healing. GC-CDs were synthesized through a simple and environmentally friendly microwave-assisted method. Comprehensive physicochemical characterizations were performed using Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Photoelectron Spectroscopy (XPS). The results revealed that GC-CDs exhibit a quasi-spherical structure, excellent water solubility, and an average particle size of 1.02 nm. Furthermore, \u003cem\u003ein vitro\u003c/em\u003e cytotoxicity assays, hemolysis tests, and \u003cem\u003ein vivo\u003c/em\u003e toxicity evaluations demonstrated the good biocompatibility of GC-CDs. Additionally, GC-CDs effectively inhibited the growth of \u003cem\u003eEscherichia coli\u003c/em\u003e (\u003cem\u003eE. coli\u003c/em\u003e) and exhibited significant wound healing promotion effects in cell scratch assays, Transwell migration tests, and mouse wound models. This study successfully demonstrated the excellent antibacterial properties and wound-healing efficacy of GC-CDs while revealing their potential ecological value in transforming waste into valuable resources. These findings provide an innovative strategy for the eco-friendly utilization of pest resources, such as longhorn beetles.\u003c/p\u003e","manuscriptTitle":"Antibacterial activity and wound healing promotion of biomass carbon dots derived from a forestry pest, Glenea cantor (Coleoptera: Cerambycidae)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-06-02 17:11:40","doi":"10.21203/rs.3.rs-6747273/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-06-09T15:53:23+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-06-07T03:20:10+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-06-05T09:49:16+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"127761123749133015624973723532177060002","date":"2025-06-05T07:06:36+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-06-01T09:11:53+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"3602030236578113491333300208542755681","date":"2025-05-30T11:16:28+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"117302245047760426748128747359236675457","date":"2025-05-30T11:01:06+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"258755070297253312777293296342479460232","date":"2025-05-30T10:46:14+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-05-30T10:37:04+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-05-29T16:24:14+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-05-29T10:19:38+00:00","index":"","fulltext":""},{"type":"submitted","content":"Chemical and Biological Technologies in Agriculture","date":"2025-05-26T05:26:32+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"chemical-and-biological-technologies-in-agriculture","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Chemical and Biological Technologies in Agriculture](https://chembioagro.springeropen.com/)","snPcode":"40538","submissionUrl":"https://submission.nature.com/new-submission/40538/3","title":"Chemical and Biological Technologies in Agriculture","twitterHandle":"@SpringerPlants","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"939c1839-ae4f-4bee-84d3-79fca82b46a0","owner":[],"postedDate":"June 2nd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2025-08-17T14:53:17+00:00","versionOfRecord":[],"versionCreatedAt":"2025-06-02 17:11:40","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6747273","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6747273","identity":"rs-6747273","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}