Nuclear movement generate binuclear cells during neural transdifferentiation from human bone marrow-derived mesenchymal cells

Nuclear movement generate binuclear cells during neural transdifferentiation from human bone marrow-derived mesenchymal cells | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Nuclear movement generate binuclear cells during neural transdifferentiation from human bone marrow-derived mesenchymal cells Carlos bueno, Miguel Blanquer, David García- Bernal, Salvador Martínez, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1707146/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Although it has been reported that bone marrow-derived mesenchymal stromal cells (BM-MSCs) can transdifferentiate into neural cells, the findings is considered unlikely. Cytotoxic-induced cell changes and cell fusion events are alternative explanations for these observations in culture and transplantation studies, respectively. Here, we show that BM-MSCs neural transdifferentiation involves a formation of dedifferentiated cells that can then redifferentiate into neural-like cells, redifferentiate back to mesenchymal fate, or even switch lineages repeatedly without cell division. Futhermore, we have discovered that nuclei from dedifferentiated cells move within the cell adopting different morphologies and even forming binucleated cells. In addition, we noted that dedifferentiated cells position their nucleus at the forefront of the cell during cell migration. Our result show that human BM-MSCs can rapidly adopt a neural-like morphology and binuclear human BM-MSCs can be formed without being related to cell fusion events. We provide additional evidences that BM-MSCs can transdifferentiate into neural cells. Stem Cell & Developmental Cell Biology stem cells neurogenesis nuclear remodelling transdifferentiation Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1707146","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":109730490,"identity":"49f67955-f4e5-4ffb-9213-354939c7486c","order_by":0,"name":"Carlos bueno","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAz0lEQVRIiWNgGAWjYHACxgNAIgHM/ECsHrgWxhkka2HmIUY5f/vZBwc+/GHIMzjee/Cxbds2Bn7+A/i1SJxJNzg4s42h2ODMuWTj3LbbDJIzEvBrMWBIYzjM28CQuOFGjpk0SIvBDQIOM+B/xnCY5w9Qy/03ZtKWQC325wk4zEACaAsPG8gWHjNpRpAtDAQcJnHjGQPQLxLFkmdyjA17zt3mkbhBQAt/fxrjgw9/bPL4jp8xfPCj7LYcfz8Bh8Esg7OIippRMApGwSgYBQQAAM+iRCK3j29eAAAAAElFTkSuQmCC","orcid":"","institution":"","correspondingAuthor":true,"prefix":"","firstName":"Carlos","middleName":"","lastName":"bueno","suffix":""},{"id":109730516,"identity":"0403263f-11c7-4e38-a236-302b79f38991","order_by":1,"name":"Miguel Blanquer","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Miguel","middleName":"","lastName":"Blanquer","suffix":""},{"id":109730585,"identity":"d88dd22f-5763-41b3-bc7e-a16d388b91ff","order_by":2,"name":"David García- Bernal","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"David","middleName":"García-","lastName":"Bernal","suffix":""},{"id":109730586,"identity":"ca7b803f-f1b9-4254-9ad3-c706d84a8f50","order_by":3,"name":"Salvador Martínez","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Salvador","middleName":"","lastName":"Martínez","suffix":""},{"id":109730587,"identity":"90cccbb7-7fb9-45a4-8a0d-726015b6482c","order_by":4,"name":"José M Moraleda","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"José","middleName":"M","lastName":"Moraleda","suffix":""}],"badges":[],"createdAt":"2022-05-30 09:00:27","currentVersionCode":1,"declarations":{"humanSubjects":true,"vertebrateSubjects":false,"conflictsOfInterestStatement":true,"humanSubjectEthicalGuidelines":true,"humanSubjectConsent":true,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false,"coiExplicitlySet":false},"doi":"10.21203/rs.3.rs-1707146/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1707146/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":22075408,"identity":"9fcf216f-cded-4062-9367-bbaba1911972","added_by":"auto","created_at":"2022-05-31 15:16:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":3448675,"visible":true,"origin":"","legend":"","description":"","filename":"ARTICULO2022MANDARPREPRINT.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1707146/v1_covered.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eNuclear movement generate binuclear cells during neural transdifferentiation from human bone marrow-derived mesenchymal cells\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-1707146/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"stem cells, neurogenesis, nuclear remodelling, transdifferentiation","lastPublishedDoi":"10.21203/rs.3.rs-1707146/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1707146/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eAlthough it has been reported that bone marrow-derived mesenchymal stromal cells (BM-MSCs) can transdifferentiate into neural cells, the findings is considered unlikely. Cytotoxic-induced cell changes and cell fusion events are alternative explanations for these observations in culture and transplantation studies, respectively.\u003c/p\u003e\u003cp\u003eHere, we show that BM-MSCs neural transdifferentiation involves a formation of dedifferentiated cells that can then redifferentiate into neural-like cells, redifferentiate back to mesenchymal fate, or even switch lineages repeatedly without cell division. Futhermore, we have discovered that nuclei from dedifferentiated cells move within the cell adopting different morphologies and even forming binucleated cells. In addition, we noted that dedifferentiated cells position their nucleus at the forefront of the cell during cell migration.\u003c/p\u003e\u003cp\u003eOur result show that human BM-MSCs can rapidly adopt a neural-like morphology and binuclear human BM-MSCs can be formed without being related to cell fusion events. 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